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Flowsheet Modelling and Simulation of Aqueous Two-Phase System for Protein Extraction

Aqueous two-phase extraction system (ATPES) has many advantages as an efficient, inexpensive large-scale liquid-liquid extraction technique for protein separation: it provides mild environment for proteins, it can be integrated with upstream processes, it can handle high throughput, it is highly sel...

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Main Author: Ahmad, Murni M
Format: Conference or Workshop Item
Published: 2007
Subjects:
TP Chemical technology
Online Access:http://eprints.utp.edu.my/3123/1/RSCE2007.docx
http://eprints.utp.edu.my/3123/
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spelling my.utp.eprints.31232017-01-19T08:26:49Z Flowsheet Modelling and Simulation of Aqueous Two-Phase System for Protein Extraction Ahmad, Murni M TP Chemical technology Aqueous two-phase extraction system (ATPES) has many advantages as an efficient, inexpensive large-scale liquid-liquid extraction technique for protein separation: it provides mild environment for proteins, it can be integrated with upstream processes, it can handle high throughput, it is highly selective hence offering high yield, and most importantly, it costs less comparatively to other conventional technique. However, the realization of ATPES as a new protein separation technology at industrial scales is rather limited. Among the reasons are that the selection of phase-forming agents can be exhaustive as there are many tuneable parameters, efforts are mostly empirical and intuitive with many possibilities and there are limited design approaches capable of accurate prediction of system and product behaviour that offer large scope of application with operation assessment and performance sensitivity available for ATPES. This paper describes the framework we designed to calculate suitable flowsheets for the extraction of a specified target protein from a complex protein feed using ATPES. We set up flowsheets according to target protein partitioning behavior in ATPES and calculated the amount of phase-forming components needed to extract the target protein from the complex mixture. Extraction units consisted of either one or two stages containing an ATPES. The thermodynamics of phase formation and partitioning were modeled using Flory-Huggins theory and calculated based on a non-linear minimization of Gibbs energy difference approach. For a case study application, we present the design of suitable flowsheets for a model feedstock containing phosphofructokinase and demonstrate the existence of feasible solutions. The ATPESs we consider are the water-PEG6000-DxT500 and water-PEG6000-Na3PO4 systems for which we developed phase behavior model. We discuss some scaling issues involved in the thermodynamic calculations and propose scaling weights and problem reformulation approaches to overcome computational challenges. We then compare the flowsheets in terms of their performance, settling rate and cost factor. We also analyze the performance trends qualitatively to check that the flowsheet calculation results capture the physical behavior of the unit. Finally, we propose a basis for flowsheet optimization for protein extraction using ATPES. 2007-12-04 Conference or Workshop Item PeerReviewed application/pdf http://eprints.utp.edu.my/3123/1/RSCE2007.docx Ahmad, Murni M (2007) Flowsheet Modelling and Simulation of Aqueous Two-Phase System for Protein Extraction. In: 14th Regional Symposium on Chemical Engineering, 4-5 December 2007, Gadjah, Indonesia. http://eprints.utp.edu.my/3123/
institution Universiti Teknologi Petronas
building UTP Resource Centre
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Petronas
content_source UTP Institutional Repository
url_provider http://eprints.utp.edu.my/
topic TP Chemical technology
spellingShingle TP Chemical technology
Ahmad, Murni M
Flowsheet Modelling and Simulation of Aqueous Two-Phase System for Protein Extraction
description Aqueous two-phase extraction system (ATPES) has many advantages as an efficient, inexpensive large-scale liquid-liquid extraction technique for protein separation: it provides mild environment for proteins, it can be integrated with upstream processes, it can handle high throughput, it is highly selective hence offering high yield, and most importantly, it costs less comparatively to other conventional technique. However, the realization of ATPES as a new protein separation technology at industrial scales is rather limited. Among the reasons are that the selection of phase-forming agents can be exhaustive as there are many tuneable parameters, efforts are mostly empirical and intuitive with many possibilities and there are limited design approaches capable of accurate prediction of system and product behaviour that offer large scope of application with operation assessment and performance sensitivity available for ATPES. This paper describes the framework we designed to calculate suitable flowsheets for the extraction of a specified target protein from a complex protein feed using ATPES. We set up flowsheets according to target protein partitioning behavior in ATPES and calculated the amount of phase-forming components needed to extract the target protein from the complex mixture. Extraction units consisted of either one or two stages containing an ATPES. The thermodynamics of phase formation and partitioning were modeled using Flory-Huggins theory and calculated based on a non-linear minimization of Gibbs energy difference approach. For a case study application, we present the design of suitable flowsheets for a model feedstock containing phosphofructokinase and demonstrate the existence of feasible solutions. The ATPESs we consider are the water-PEG6000-DxT500 and water-PEG6000-Na3PO4 systems for which we developed phase behavior model. We discuss some scaling issues involved in the thermodynamic calculations and propose scaling weights and problem reformulation approaches to overcome computational challenges. We then compare the flowsheets in terms of their performance, settling rate and cost factor. We also analyze the performance trends qualitatively to check that the flowsheet calculation results capture the physical behavior of the unit. Finally, we propose a basis for flowsheet optimization for protein extraction using ATPES.
format Conference or Workshop Item
author Ahmad, Murni M
author_facet Ahmad, Murni M
author_sort Ahmad, Murni M
title Flowsheet Modelling and Simulation of Aqueous Two-Phase System for Protein Extraction
title_short Flowsheet Modelling and Simulation of Aqueous Two-Phase System for Protein Extraction
title_full Flowsheet Modelling and Simulation of Aqueous Two-Phase System for Protein Extraction
title_fullStr Flowsheet Modelling and Simulation of Aqueous Two-Phase System for Protein Extraction
title_full_unstemmed Flowsheet Modelling and Simulation of Aqueous Two-Phase System for Protein Extraction
title_sort flowsheet modelling and simulation of aqueous two-phase system for protein extraction
publishDate 2007
url http://eprints.utp.edu.my/3123/1/RSCE2007.docx
http://eprints.utp.edu.my/3123/
_version_ 1738655245727170560
score 13.211869

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