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Enhanced anthraquinones production from adsorbent-treated Morinda elliptica cell suspension cultures in production medium strategy

Continuous removal of anthraquinones (AQ) by Amberlite polymeric adsorbents (XAD-4, XAD-7 and XAD-16) through in situ adsorption in Morinda elliptica cell suspension cultures is studied for product recovery and improvement of the overall titre. Ethanol was the best eluting solvent for effective reco...

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Main Authors: M.A., Abdullah, L., Chiang
Format: Article
Published: 2007
Subjects:
TP Chemical technology
Online Access:http://eprints.utp.edu.my/2862/1/Enhanced-anthraquinones-production-from-adsorbent-treated-Morinda-elliptica-cell-suspension-cultures-in-production-medium-strategy_2007_Process-Biochemistry.pdf
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spelling my.utp.eprints.28622017-01-19T08:27:06Z Enhanced anthraquinones production from adsorbent-treated Morinda elliptica cell suspension cultures in production medium strategy M.A., Abdullah L., Chiang TP Chemical technology Continuous removal of anthraquinones (AQ) by Amberlite polymeric adsorbents (XAD-4, XAD-7 and XAD-16) through in situ adsorption in Morinda elliptica cell suspension cultures is studied for product recovery and improvement of the overall titre. Ethanol was the best eluting solvent for effective recovery of AQ from all adsorbents. Pre-treatment of XAD-4 with sodium acetate not only enhanced intracellular AQ, but also AQ release and subsequent recovery from the adsorbent. The addition of sodium acetate pretreated XAD-4 on day 18 for 6-day contact period, achieved comparable cell growth to control (41 g/L), but with 1.3-fold higher intracellular AQ (124 mg/g DW) and two-fold increase in extracellular AQ (14.3 mg/L). High amount of adsorbent and longer contact period for the cultures entering stationary growth phase, stimulated AQ release and recovery but at the expense of cell growth. With 5-8.3 g XAD-4 adsorbent per litre M. elliptica culture in production (P) medium, between 60 and 90% AQ was recovered from extracellular AQ after 24-26 days of culture period. © 2007 Elsevier Ltd. All rights reserved. 2007 Article PeerReviewed application/pdf http://eprints.utp.edu.my/2862/1/Enhanced-anthraquinones-production-from-adsorbent-treated-Morinda-elliptica-cell-suspension-cultures-in-production-medium-strategy_2007_Process-Biochemistry.pdf http://www.scopus.com/inward/record.url?eid=2-s2.0-34047104562&partnerID=40&md5=dbcbb1c43e429828f8b0003089f80106 M.A., Abdullah and L., Chiang (2007) Enhanced anthraquinones production from adsorbent-treated Morinda elliptica cell suspension cultures in production medium strategy. Process Biochemistry, 42 (5). pp. 757-763. ISSN 13595113 http://eprints.utp.edu.my/2862/
institution Universiti Teknologi Petronas
building UTP Resource Centre
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Petronas
content_source UTP Institutional Repository
url_provider http://eprints.utp.edu.my/
topic TP Chemical technology
spellingShingle TP Chemical technology
M.A., Abdullah
L., Chiang
Enhanced anthraquinones production from adsorbent-treated Morinda elliptica cell suspension cultures in production medium strategy
description Continuous removal of anthraquinones (AQ) by Amberlite polymeric adsorbents (XAD-4, XAD-7 and XAD-16) through in situ adsorption in Morinda elliptica cell suspension cultures is studied for product recovery and improvement of the overall titre. Ethanol was the best eluting solvent for effective recovery of AQ from all adsorbents. Pre-treatment of XAD-4 with sodium acetate not only enhanced intracellular AQ, but also AQ release and subsequent recovery from the adsorbent. The addition of sodium acetate pretreated XAD-4 on day 18 for 6-day contact period, achieved comparable cell growth to control (41 g/L), but with 1.3-fold higher intracellular AQ (124 mg/g DW) and two-fold increase in extracellular AQ (14.3 mg/L). High amount of adsorbent and longer contact period for the cultures entering stationary growth phase, stimulated AQ release and recovery but at the expense of cell growth. With 5-8.3 g XAD-4 adsorbent per litre M. elliptica culture in production (P) medium, between 60 and 90% AQ was recovered from extracellular AQ after 24-26 days of culture period. © 2007 Elsevier Ltd. All rights reserved.
format Article
author M.A., Abdullah
L., Chiang
author_facet M.A., Abdullah
L., Chiang
author_sort M.A., Abdullah
title Enhanced anthraquinones production from adsorbent-treated Morinda elliptica cell suspension cultures in production medium strategy
title_short Enhanced anthraquinones production from adsorbent-treated Morinda elliptica cell suspension cultures in production medium strategy
title_full Enhanced anthraquinones production from adsorbent-treated Morinda elliptica cell suspension cultures in production medium strategy
title_fullStr Enhanced anthraquinones production from adsorbent-treated Morinda elliptica cell suspension cultures in production medium strategy
title_full_unstemmed Enhanced anthraquinones production from adsorbent-treated Morinda elliptica cell suspension cultures in production medium strategy
title_sort enhanced anthraquinones production from adsorbent-treated morinda elliptica cell suspension cultures in production medium strategy
publishDate 2007
url http://eprints.utp.edu.my/2862/1/Enhanced-anthraquinones-production-from-adsorbent-treated-Morinda-elliptica-cell-suspension-cultures-in-production-medium-strategy_2007_Process-Biochemistry.pdf
http://www.scopus.com/inward/record.url?eid=2-s2.0-34047104562&partnerID=40&md5=dbcbb1c43e429828f8b0003089f80106
http://eprints.utp.edu.my/2862/
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score 13.211869

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