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Determination of biogenic amines in seawater using capillary electrophoresis with capacitively coupled contactless conductivity detection

A rapid and green analytical method based on capillary electrophoresis with capacitively coupled contactless conductivity detection (C4D) for the determination of eight environmental pollutants, the biogenic amines (putrescine, cadaverine, spermidine, spermine, tyramine, 2-phenylamine, histamine and...

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Main Authors: Gubartallah, E.A., Makahleh, A., Quirino, J.P., Saad, B.
Format: Article
Published: MDPI AG 2018
Online Access:https://www.scopus.com/inward/record.uri?eid=2-s2.0-85046678013&doi=10.3390%2fmolecules23051112&partnerID=40&md5=fbea9df6ef00f0e44877e0851ee9ee19
http://eprints.utp.edu.my/22028/
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spelling my.utp.eprints.220282019-02-20T06:56:24Z Determination of biogenic amines in seawater using capillary electrophoresis with capacitively coupled contactless conductivity detection Gubartallah, E.A. Makahleh, A. Quirino, J.P. Saad, B. A rapid and green analytical method based on capillary electrophoresis with capacitively coupled contactless conductivity detection (C4D) for the determination of eight environmental pollutants, the biogenic amines (putrescine, cadaverine, spermidine, spermine, tyramine, 2-phenylamine, histamine and tryptamine), is described. The separation was achieved under normal polarity mode at 24 �C and 25 kV with a hydrodynamic injection (50 mbar for 5 s) and using a bare fused-silica capillary (95 cm length � 50 µm i.d.) (detection length of 10.5 cm from the outlet end of the capillary). The optimized background electrolyte consisted of 400 mM malic acid. C4D parameters were set at a fixed amplitude (50 V) and frequency (600 kHz). Under the optimum conditions, the method exhibited good linearity over the range of 1.0�100 µg mL� 1 (R2 � 0.981). The limits of detection based on signal to noise (S/N) ratios of 3 and 10 were �0.029 µg mL� 1. The method was used for the determination of seawater samples that were spiked with biogenic amines. Good recoveries (77�93) were found. © 2018 by the authors. MDPI AG 2018 Article PeerReviewed https://www.scopus.com/inward/record.uri?eid=2-s2.0-85046678013&doi=10.3390%2fmolecules23051112&partnerID=40&md5=fbea9df6ef00f0e44877e0851ee9ee19 Gubartallah, E.A. and Makahleh, A. and Quirino, J.P. and Saad, B. (2018) Determination of biogenic amines in seawater using capillary electrophoresis with capacitively coupled contactless conductivity detection. Molecules, 23 (5). http://eprints.utp.edu.my/22028/
institution Universiti Teknologi Petronas
building UTP Resource Centre
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Petronas
content_source UTP Institutional Repository
url_provider http://eprints.utp.edu.my/
description A rapid and green analytical method based on capillary electrophoresis with capacitively coupled contactless conductivity detection (C4D) for the determination of eight environmental pollutants, the biogenic amines (putrescine, cadaverine, spermidine, spermine, tyramine, 2-phenylamine, histamine and tryptamine), is described. The separation was achieved under normal polarity mode at 24 �C and 25 kV with a hydrodynamic injection (50 mbar for 5 s) and using a bare fused-silica capillary (95 cm length � 50 µm i.d.) (detection length of 10.5 cm from the outlet end of the capillary). The optimized background electrolyte consisted of 400 mM malic acid. C4D parameters were set at a fixed amplitude (50 V) and frequency (600 kHz). Under the optimum conditions, the method exhibited good linearity over the range of 1.0�100 µg mL� 1 (R2 � 0.981). The limits of detection based on signal to noise (S/N) ratios of 3 and 10 were �0.029 µg mL� 1. The method was used for the determination of seawater samples that were spiked with biogenic amines. Good recoveries (77�93) were found. © 2018 by the authors.
format Article
author Gubartallah, E.A.
Makahleh, A.
Quirino, J.P.
Saad, B.
spellingShingle Gubartallah, E.A.
Makahleh, A.
Quirino, J.P.
Saad, B.
Determination of biogenic amines in seawater using capillary electrophoresis with capacitively coupled contactless conductivity detection
author_facet Gubartallah, E.A.
Makahleh, A.
Quirino, J.P.
Saad, B.
author_sort Gubartallah, E.A.
title Determination of biogenic amines in seawater using capillary electrophoresis with capacitively coupled contactless conductivity detection
title_short Determination of biogenic amines in seawater using capillary electrophoresis with capacitively coupled contactless conductivity detection
title_full Determination of biogenic amines in seawater using capillary electrophoresis with capacitively coupled contactless conductivity detection
title_fullStr Determination of biogenic amines in seawater using capillary electrophoresis with capacitively coupled contactless conductivity detection
title_full_unstemmed Determination of biogenic amines in seawater using capillary electrophoresis with capacitively coupled contactless conductivity detection
title_sort determination of biogenic amines in seawater using capillary electrophoresis with capacitively coupled contactless conductivity detection
publisher MDPI AG
publishDate 2018
url https://www.scopus.com/inward/record.uri?eid=2-s2.0-85046678013&doi=10.3390%2fmolecules23051112&partnerID=40&md5=fbea9df6ef00f0e44877e0851ee9ee19
http://eprints.utp.edu.my/22028/
_version_ 1738656371892551680
score 13.211869

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