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Genomic analysis for haloacid dehalogenase in bacillus megaterium WSH-002

Bacterial dehalogenation is one of the processes that can reduce environmental pol-lutions. The attributes of B. megaterium that can grow in a polluted environment suggested that its genome contains pollutant degrading genes. To date, there were no reports related to dehalogenase in B. megaterium WS...

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Main Authors: Zulkarnain, Luqman Arif, Huyop, Fahrul
Format: Article
Language:English
Published: Brawijaya University 2022
Subjects:
Q Science (General)
Online Access:http://eprints.utm.my/id/eprint/98776/1/LuqmanArifZulkarnain2022_GenomicAnalysisforHaloacidDehalogenase.pdf
http://eprints.utm.my/id/eprint/98776/
http://dx.doi.org/10.11594/jtls.12.01.07
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spelling my.utm.987762023-02-02T08:40:46Z http://eprints.utm.my/id/eprint/98776/ Genomic analysis for haloacid dehalogenase in bacillus megaterium WSH-002 Zulkarnain, Luqman Arif Huyop, Fahrul Q Science (General) Bacterial dehalogenation is one of the processes that can reduce environmental pol-lutions. The attributes of B. megaterium that can grow in a polluted environment suggested that its genome contains pollutant degrading genes. To date, there were no reports related to dehalogenase in B. megaterium WSH-002 and how it was regulated. Therefore, the presence of environmentally important genes that can de-toxify organohalogens in many microbial genomes, including B. megaterium WSH-002 will be investigated. The genome sequence of B. megaterium WSH-002 was retrieved from NCBI databases. It was then annotated through the RAST server to identify all the putative dehalogenase gene sequences. The selected gene sequence was converted into amino and went through BLASTp via UniProt data-base. The highest percentage identity of the amino acid sequence to any dehalo-genases was subjected to further identification of specific dehalogenase domain using InterPro Scan server. The results from genome annotations have shown its potential for bioremediation due to the presence of putative dehalogenase protein. Only one type of haloacid dehalogenase was identified. It was classified as haloacid dehalogenase type II because its amino acid sequence is highly identical with HAD_type_II and HAD_L2-DEX. The study concluded that the genome of B. megaterium WSH-002 contains a haloacid dehalogenase gene that is useful for the biodegradation of halogenated compounds. In the future, further investigation on the expression of the dehalogenase gene as recombinant protein and to study its protein structure and functions will be considered. Brawijaya University 2022-02 Article PeerReviewed application/pdf en http://eprints.utm.my/id/eprint/98776/1/LuqmanArifZulkarnain2022_GenomicAnalysisforHaloacidDehalogenase.pdf Zulkarnain, Luqman Arif and Huyop, Fahrul (2022) Genomic analysis for haloacid dehalogenase in bacillus megaterium WSH-002. Journal of Tropical Life Science, 12 (1). pp. 73-82. ISSN 2087-5517 http://dx.doi.org/10.11594/jtls.12.01.07 DOI: 10.11594/jtls.12.01.07
institution Universiti Teknologi Malaysia
building UTM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Malaysia
content_source UTM Institutional Repository
url_provider http://eprints.utm.my/
language English
topic Q Science (General)
spellingShingle Q Science (General)
Zulkarnain, Luqman Arif
Huyop, Fahrul
Genomic analysis for haloacid dehalogenase in bacillus megaterium WSH-002
description Bacterial dehalogenation is one of the processes that can reduce environmental pol-lutions. The attributes of B. megaterium that can grow in a polluted environment suggested that its genome contains pollutant degrading genes. To date, there were no reports related to dehalogenase in B. megaterium WSH-002 and how it was regulated. Therefore, the presence of environmentally important genes that can de-toxify organohalogens in many microbial genomes, including B. megaterium WSH-002 will be investigated. The genome sequence of B. megaterium WSH-002 was retrieved from NCBI databases. It was then annotated through the RAST server to identify all the putative dehalogenase gene sequences. The selected gene sequence was converted into amino and went through BLASTp via UniProt data-base. The highest percentage identity of the amino acid sequence to any dehalo-genases was subjected to further identification of specific dehalogenase domain using InterPro Scan server. The results from genome annotations have shown its potential for bioremediation due to the presence of putative dehalogenase protein. Only one type of haloacid dehalogenase was identified. It was classified as haloacid dehalogenase type II because its amino acid sequence is highly identical with HAD_type_II and HAD_L2-DEX. The study concluded that the genome of B. megaterium WSH-002 contains a haloacid dehalogenase gene that is useful for the biodegradation of halogenated compounds. In the future, further investigation on the expression of the dehalogenase gene as recombinant protein and to study its protein structure and functions will be considered.
format Article
author Zulkarnain, Luqman Arif
Huyop, Fahrul
author_facet Zulkarnain, Luqman Arif
Huyop, Fahrul
author_sort Zulkarnain, Luqman Arif
title Genomic analysis for haloacid dehalogenase in bacillus megaterium WSH-002
title_short Genomic analysis for haloacid dehalogenase in bacillus megaterium WSH-002
title_full Genomic analysis for haloacid dehalogenase in bacillus megaterium WSH-002
title_fullStr Genomic analysis for haloacid dehalogenase in bacillus megaterium WSH-002
title_full_unstemmed Genomic analysis for haloacid dehalogenase in bacillus megaterium WSH-002
title_sort genomic analysis for haloacid dehalogenase in bacillus megaterium wsh-002
publisher Brawijaya University
publishDate 2022
url http://eprints.utm.my/id/eprint/98776/1/LuqmanArifZulkarnain2022_GenomicAnalysisforHaloacidDehalogenase.pdf
http://eprints.utm.my/id/eprint/98776/
http://dx.doi.org/10.11594/jtls.12.01.07
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score 13.211869

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