Cover Image

Purification of Fim-C-Salmonella typhi recombinant protein with Ni-NTA resin as raw material for typhoid disease detection kit

Typhoid fever caused by Salmonella typhi is an endemic disease in Asia, including Indonesia. Based on data from the World Health Organization (WHO), there are 81% per 100,000 people in Indonesia infected with typhoid. Based on this, development of typhoid detection kit that is simple, fast, specific...

Full description

Saved in:
Bibliographic Details
Main Authors: Nurjayadi, M., Setiyoto, T., Jinan, S. F., Hardianto, D., Sulfianti, A., Agustini, K., El-Enshasy, H. A.
Format: Conference or Workshop Item
Language:English
Published: 2021
Subjects:
TP Chemical technology
Online Access:http://eprints.utm.my/id/eprint/98079/1/HeshamAliMetwally2021_PurificationofFimCSalmonellaTyphiRecombinantProtein.pdf
http://eprints.utm.my/id/eprint/98079/
http://dx.doi.org/10.1088/1742-6596/1869/1/012033
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.utm.98079
record_format eprints
spelling my.utm.980792022-11-30T04:07:14Z http://eprints.utm.my/id/eprint/98079/ Purification of Fim-C-Salmonella typhi recombinant protein with Ni-NTA resin as raw material for typhoid disease detection kit Nurjayadi, M. Setiyoto, T. Jinan, S. F. Hardianto, D. Sulfianti, A. Agustini, K. El-Enshasy, H. A. TP Chemical technology Typhoid fever caused by Salmonella typhi is an endemic disease in Asia, including Indonesia. Based on data from the World Health Organization (WHO), there are 81% per 100,000 people in Indonesia infected with typhoid. Based on this, development of typhoid detection kit that is simple, fast, specific and sensitive is still needed. One of the developments of detection kits currently available is the antigen and antibody interaction approach. This study aims to obtain comprehensive information about purification of recombinant Fim-C-S. typhi proteins as antigens for raw materials for manufacturing typhoid detection kits. This study uses the IMAC method with Ni2+ metal, which has advantages in high selectivity and purity. The results of this study report that purification of recombinant Fim-C-S. typhi protein inclusion bodies with variations in the binding and washing process at 3, 5, and 7 times gave percent yields correspondingly of 37.84, 39.44, and 38.21% pure protein. Previous studies have also reported variations in binding and washing at 2, 4, and 6 times and gave percent yields respectively were 35.37, 39.11, and 40.49%. Based on the data, we concluded that the variation of 6 times is the best repetition variation to get the largest percent yield so that it can be used as a reference in large-scale purification. 2021 Conference or Workshop Item PeerReviewed application/pdf en http://eprints.utm.my/id/eprint/98079/1/HeshamAliMetwally2021_PurificationofFimCSalmonellaTyphiRecombinantProtein.pdf Nurjayadi, M. and Setiyoto, T. and Jinan, S. F. and Hardianto, D. and Sulfianti, A. and Agustini, K. and El-Enshasy, H. A. (2021) Purification of Fim-C-Salmonella typhi recombinant protein with Ni-NTA resin as raw material for typhoid disease detection kit. In: 2nd Annual Conference of Science and Technology, ANCOSET 2020, 28 November 2020, Malang, Virtual. http://dx.doi.org/10.1088/1742-6596/1869/1/012033
institution Universiti Teknologi Malaysia
building UTM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Malaysia
content_source UTM Institutional Repository
url_provider http://eprints.utm.my/
language English
topic TP Chemical technology
spellingShingle TP Chemical technology
Nurjayadi, M.
Setiyoto, T.
Jinan, S. F.
Hardianto, D.
Sulfianti, A.
Agustini, K.
El-Enshasy, H. A.
Purification of Fim-C-Salmonella typhi recombinant protein with Ni-NTA resin as raw material for typhoid disease detection kit
description Typhoid fever caused by Salmonella typhi is an endemic disease in Asia, including Indonesia. Based on data from the World Health Organization (WHO), there are 81% per 100,000 people in Indonesia infected with typhoid. Based on this, development of typhoid detection kit that is simple, fast, specific and sensitive is still needed. One of the developments of detection kits currently available is the antigen and antibody interaction approach. This study aims to obtain comprehensive information about purification of recombinant Fim-C-S. typhi proteins as antigens for raw materials for manufacturing typhoid detection kits. This study uses the IMAC method with Ni2+ metal, which has advantages in high selectivity and purity. The results of this study report that purification of recombinant Fim-C-S. typhi protein inclusion bodies with variations in the binding and washing process at 3, 5, and 7 times gave percent yields correspondingly of 37.84, 39.44, and 38.21% pure protein. Previous studies have also reported variations in binding and washing at 2, 4, and 6 times and gave percent yields respectively were 35.37, 39.11, and 40.49%. Based on the data, we concluded that the variation of 6 times is the best repetition variation to get the largest percent yield so that it can be used as a reference in large-scale purification.
format Conference or Workshop Item
author Nurjayadi, M.
Setiyoto, T.
Jinan, S. F.
Hardianto, D.
Sulfianti, A.
Agustini, K.
El-Enshasy, H. A.
author_facet Nurjayadi, M.
Setiyoto, T.
Jinan, S. F.
Hardianto, D.
Sulfianti, A.
Agustini, K.
El-Enshasy, H. A.
author_sort Nurjayadi, M.
title Purification of Fim-C-Salmonella typhi recombinant protein with Ni-NTA resin as raw material for typhoid disease detection kit
title_short Purification of Fim-C-Salmonella typhi recombinant protein with Ni-NTA resin as raw material for typhoid disease detection kit
title_full Purification of Fim-C-Salmonella typhi recombinant protein with Ni-NTA resin as raw material for typhoid disease detection kit
title_fullStr Purification of Fim-C-Salmonella typhi recombinant protein with Ni-NTA resin as raw material for typhoid disease detection kit
title_full_unstemmed Purification of Fim-C-Salmonella typhi recombinant protein with Ni-NTA resin as raw material for typhoid disease detection kit
title_sort purification of fim-c-salmonella typhi recombinant protein with ni-nta resin as raw material for typhoid disease detection kit
publishDate 2021
url http://eprints.utm.my/id/eprint/98079/1/HeshamAliMetwally2021_PurificationofFimCSalmonellaTyphiRecombinantProtein.pdf
http://eprints.utm.my/id/eprint/98079/
http://dx.doi.org/10.1088/1742-6596/1869/1/012033
_version_ 1751536144545218560
score 13.211869

Similar Items