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Purification and identification of rotenone from derris elliptica by using the vacuum liquid chromatography-thin layer chromatography (VLC-TLC) method

History has recorded the use of rotenone as bio-pesticide by plant and vegetables growers worldwide. The reintroduction of this bio-active compound as an environmental friendly bio-pesticide in organic fruits and vegetables plantations is now an emergent trend. Our laboratory is focused on the devel...

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Main Authors: Zubairi, Saiful Irwan, Sarmidi, M. R., Aziz, R. A., Ramli, M. K. A., Latip, R., Nordin, N. I. A.
Format: Conference or Workshop Item
Language:English
Published: 2004
Subjects:
TP Chemical technology
QH301 Biology
Online Access:http://eprints.utm.my/id/eprint/9799/1/ZubairiSI2004_purification_and_identification_of_rotenone.pdf
http://eprints.utm.my/id/eprint/9799/
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spelling my.utm.97992010-06-02T02:03:32Z http://eprints.utm.my/id/eprint/9799/ Purification and identification of rotenone from derris elliptica by using the vacuum liquid chromatography-thin layer chromatography (VLC-TLC) method Zubairi, Saiful Irwan Sarmidi, M. R. Aziz, R. A. Ramli, M. K. A. Latip, R. Nordin, N. I. A. TP Chemical technology QH301 Biology History has recorded the use of rotenone as bio-pesticide by plant and vegetables growers worldwide. The reintroduction of this bio-active compound as an environmental friendly bio-pesticide in organic fruits and vegetables plantations is now an emergent trend. Our laboratory is focused on the development of bio-pesticide using rotenone extracted from local Derris elliptica. Liquid chromatographic method with high vacuum pressure was developed for the analysis of rotenone in the crude extract of Derris elliptica. The treated root and stem were cut into small pieces prior to extraction process using the Normal Soaking Extraction (NSE) method. The crude extract was concentrated further by using rotary evaporator at 40 0C under reduced pressure of 800 mbar. A high-pressure vacuum liquid chromatographic was then carried out for the purification of rotenone using silica gel (230 mesh to 400 mesh) with variety of eluents polarity (hexane, chloroform, acetone and ultra pure water respectively). All eluents that have been collected after the purification process were subjected to the semi-automatic TLC (CAMAG Linomat 5) for the identification of rotenone and other constituents by using an isocratic elution with a mixture of petroleum ether and ethyl acetate with a ratio of 4:2. The markers for each bio-active constituent were visualized and recorded using UV lamp (wavelength of 254 nm and 365 nm) and the Rf values for desirable constituent (rotenone) was calculated respectively. The employed method shows significant improvement in rotenone separation and purification compared to the normal TLC method. 2004-08 Conference or Workshop Item PeerReviewed application/pdf en http://eprints.utm.my/id/eprint/9799/1/ZubairiSI2004_purification_and_identification_of_rotenone.pdf Zubairi, Saiful Irwan and Sarmidi, M. R. and Aziz, R. A. and Ramli, M. K. A. and Latip, R. and Nordin, N. I. A. (2004) Purification and identification of rotenone from derris elliptica by using the vacuum liquid chromatography-thin layer chromatography (VLC-TLC) method. In: Proceeding of Simposium Kimia Analisis Kebangsaan (SKAM-17), 26-28 August 2004, Swiss Garden Resort, Pahang.
institution Universiti Teknologi Malaysia
building UTM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Malaysia
content_source UTM Institutional Repository
url_provider http://eprints.utm.my/
language English
topic TP Chemical technology
QH301 Biology
spellingShingle TP Chemical technology
QH301 Biology
Zubairi, Saiful Irwan
Sarmidi, M. R.
Aziz, R. A.
Ramli, M. K. A.
Latip, R.
Nordin, N. I. A.
Purification and identification of rotenone from derris elliptica by using the vacuum liquid chromatography-thin layer chromatography (VLC-TLC) method
description History has recorded the use of rotenone as bio-pesticide by plant and vegetables growers worldwide. The reintroduction of this bio-active compound as an environmental friendly bio-pesticide in organic fruits and vegetables plantations is now an emergent trend. Our laboratory is focused on the development of bio-pesticide using rotenone extracted from local Derris elliptica. Liquid chromatographic method with high vacuum pressure was developed for the analysis of rotenone in the crude extract of Derris elliptica. The treated root and stem were cut into small pieces prior to extraction process using the Normal Soaking Extraction (NSE) method. The crude extract was concentrated further by using rotary evaporator at 40 0C under reduced pressure of 800 mbar. A high-pressure vacuum liquid chromatographic was then carried out for the purification of rotenone using silica gel (230 mesh to 400 mesh) with variety of eluents polarity (hexane, chloroform, acetone and ultra pure water respectively). All eluents that have been collected after the purification process were subjected to the semi-automatic TLC (CAMAG Linomat 5) for the identification of rotenone and other constituents by using an isocratic elution with a mixture of petroleum ether and ethyl acetate with a ratio of 4:2. The markers for each bio-active constituent were visualized and recorded using UV lamp (wavelength of 254 nm and 365 nm) and the Rf values for desirable constituent (rotenone) was calculated respectively. The employed method shows significant improvement in rotenone separation and purification compared to the normal TLC method.
format Conference or Workshop Item
author Zubairi, Saiful Irwan
Sarmidi, M. R.
Aziz, R. A.
Ramli, M. K. A.
Latip, R.
Nordin, N. I. A.
author_facet Zubairi, Saiful Irwan
Sarmidi, M. R.
Aziz, R. A.
Ramli, M. K. A.
Latip, R.
Nordin, N. I. A.
author_sort Zubairi, Saiful Irwan
title Purification and identification of rotenone from derris elliptica by using the vacuum liquid chromatography-thin layer chromatography (VLC-TLC) method
title_short Purification and identification of rotenone from derris elliptica by using the vacuum liquid chromatography-thin layer chromatography (VLC-TLC) method
title_full Purification and identification of rotenone from derris elliptica by using the vacuum liquid chromatography-thin layer chromatography (VLC-TLC) method
title_fullStr Purification and identification of rotenone from derris elliptica by using the vacuum liquid chromatography-thin layer chromatography (VLC-TLC) method
title_full_unstemmed Purification and identification of rotenone from derris elliptica by using the vacuum liquid chromatography-thin layer chromatography (VLC-TLC) method
title_sort purification and identification of rotenone from derris elliptica by using the vacuum liquid chromatography-thin layer chromatography (vlc-tlc) method
publishDate 2004
url http://eprints.utm.my/id/eprint/9799/1/ZubairiSI2004_purification_and_identification_of_rotenone.pdf
http://eprints.utm.my/id/eprint/9799/
_version_ 1643645256175976448
score 13.251813

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