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In vitro regeneration of Garden Balsam, impatiens balsamina using cotyledons derived from seedlings

Garden balsam, Impatiens balsamina, is one of an important ornamental plant in Malaysia. An effort to develop a reliable tissue culture system for this Impatiens species was carried out for future genetic manipulation of this plant for useful traits. Parts of cotyledons and hypocotyl derived from di...

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Main Authors: Huyop, Fahrul Zaman, Wagiran, Alina, Mohd. Taha, Aishah, Ghazali, Hamidah
Format: Monograph
Language:English
Published: Faculty of Biosciences and Bioengineering 2009
Subjects:
Q Science (General)
Online Access:http://eprints.utm.my/id/eprint/9756/1/78180.pdf
http://eprints.utm.my/id/eprint/9756/
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spelling my.utm.97562011-05-20T07:56:46Z http://eprints.utm.my/id/eprint/9756/ In vitro regeneration of Garden Balsam, impatiens balsamina using cotyledons derived from seedlings Huyop, Fahrul Zaman Wagiran, Alina Mohd. Taha, Aishah Ghazali, Hamidah Q Science (General) Garden balsam, Impatiens balsamina, is one of an important ornamental plant in Malaysia. An effort to develop a reliable tissue culture system for this Impatiens species was carried out for future genetic manipulation of this plant for useful traits. Parts of cotyledons and hypocotyl derived from different age of seedlings, from surface sterilized seeds, were subjected to shoot induction on MS media containing BAP, TDZ or combination of BAP and NAA in different ratios. Roots were developed from the above shoots on full- or halfstrength MS media containing IAA, IBA or NAA of different concentrations. Finally fully-developed plantlets were produced on hormone free MS media or full- or half-strength MS media containing specific hormones. It was observed that the most efficient shoot induction (93%) was obtained from proximal part of cotyledon derived from 7 days old seedlings on MS media supplemented with 1mg/L BAP. Roots were most efficiently produced (92%), in term of percentage and morphology, on half-strength MS media supplemented with 0.1 mg/L IAA. Further regeneration of plantlets, approximately 8-10 cm in height, was achieved after 3 weeks on hormone-free MS media. Finally, full regeneration system of Impatiens balsamina was developed, using MS media supplemented with 1 mg/L BAP for shoot and 0.1 mg/L IAA for roots, within 8 weeks in culture. Faculty of Biosciences and Bioengineering 2009-08-31 Monograph NonPeerReviewed application/pdf en http://eprints.utm.my/id/eprint/9756/1/78180.pdf Huyop, Fahrul Zaman and Wagiran, Alina and Mohd. Taha, Aishah and Ghazali, Hamidah (2009) In vitro regeneration of Garden Balsam, impatiens balsamina using cotyledons derived from seedlings. Project Report. Faculty of Biosciences and Bioengineering, Skudai, Johor. (Unpublished)
institution Universiti Teknologi Malaysia
building UTM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Malaysia
content_source UTM Institutional Repository
url_provider http://eprints.utm.my/
language English
topic Q Science (General)
spellingShingle Q Science (General)
Huyop, Fahrul Zaman
Wagiran, Alina
Mohd. Taha, Aishah
Ghazali, Hamidah
In vitro regeneration of Garden Balsam, impatiens balsamina using cotyledons derived from seedlings
description Garden balsam, Impatiens balsamina, is one of an important ornamental plant in Malaysia. An effort to develop a reliable tissue culture system for this Impatiens species was carried out for future genetic manipulation of this plant for useful traits. Parts of cotyledons and hypocotyl derived from different age of seedlings, from surface sterilized seeds, were subjected to shoot induction on MS media containing BAP, TDZ or combination of BAP and NAA in different ratios. Roots were developed from the above shoots on full- or halfstrength MS media containing IAA, IBA or NAA of different concentrations. Finally fully-developed plantlets were produced on hormone free MS media or full- or half-strength MS media containing specific hormones. It was observed that the most efficient shoot induction (93%) was obtained from proximal part of cotyledon derived from 7 days old seedlings on MS media supplemented with 1mg/L BAP. Roots were most efficiently produced (92%), in term of percentage and morphology, on half-strength MS media supplemented with 0.1 mg/L IAA. Further regeneration of plantlets, approximately 8-10 cm in height, was achieved after 3 weeks on hormone-free MS media. Finally, full regeneration system of Impatiens balsamina was developed, using MS media supplemented with 1 mg/L BAP for shoot and 0.1 mg/L IAA for roots, within 8 weeks in culture.
format Monograph
author Huyop, Fahrul Zaman
Wagiran, Alina
Mohd. Taha, Aishah
Ghazali, Hamidah
author_facet Huyop, Fahrul Zaman
Wagiran, Alina
Mohd. Taha, Aishah
Ghazali, Hamidah
author_sort Huyop, Fahrul Zaman
title In vitro regeneration of Garden Balsam, impatiens balsamina using cotyledons derived from seedlings
title_short In vitro regeneration of Garden Balsam, impatiens balsamina using cotyledons derived from seedlings
title_full In vitro regeneration of Garden Balsam, impatiens balsamina using cotyledons derived from seedlings
title_fullStr In vitro regeneration of Garden Balsam, impatiens balsamina using cotyledons derived from seedlings
title_full_unstemmed In vitro regeneration of Garden Balsam, impatiens balsamina using cotyledons derived from seedlings
title_sort in vitro regeneration of garden balsam, impatiens balsamina using cotyledons derived from seedlings
publisher Faculty of Biosciences and Bioengineering
publishDate 2009
url http://eprints.utm.my/id/eprint/9756/1/78180.pdf
http://eprints.utm.my/id/eprint/9756/
_version_ 1643645244922658816
score 13.211869

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