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Comparative evaluation of different DNA extraction methods from E. Longifolia herbal medicinal product

The aphrodisiac property of Eurycoma longifolia has led to an increase in the demand for its Herbal Medicinal Products (HMPs). However, the efficiency of such HMPs depends on the usage of their genuine raw materials. The conventional methods cannot identify species in processed form. The authenticat...

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Main Authors: Abubakar, Bashir Mohammed, Md. Salleh, Faezah, Wagiran, Alina, Abba, Mustapha
Format: Article
Language:English
Published: John Wiley & Sons, Inc 2021
Subjects:
Q Science (General)
Online Access:http://eprints.utm.my/id/eprint/97181/1/FaezahMdSalleh2021_ComparativeEvaluationofDifferentDNA.pdf
http://eprints.utm.my/id/eprint/97181/
http://dx.doi.org/10.2991/efood.k.210202.001
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spelling my.utm.971812022-09-23T02:45:38Z http://eprints.utm.my/id/eprint/97181/ Comparative evaluation of different DNA extraction methods from E. Longifolia herbal medicinal product Abubakar, Bashir Mohammed Md. Salleh, Faezah Wagiran, Alina Abba, Mustapha Q Science (General) The aphrodisiac property of Eurycoma longifolia has led to an increase in the demand for its Herbal Medicinal Products (HMPs). However, the efficiency of such HMPs depends on the usage of their genuine raw materials. The conventional methods cannot identify species in processed form. The authentication of HMPs can be achieved effectively using DNA barcoding as the method species-specific. However, the use of this method solely relied on the extraction of high-quality DNA from the HMPs. Therefore, it is necessary to establish a satisfactory method for extracting high-quality DNA from the HMPs. Here, four DNA extraction methods were compared to evaluate the best protocol in yield, purity, polymerase chain reaction (PCR) amplification, sequencing, and species identification. The spectrophotometer analysis showed that the Nucleospin Plant II extraction kit has the best purity as this can be severely affected by the presence of various contaminants in the HMPs. Our findings reveal that DNA purity was more important as a predictor for PCR amplification than yield. Therefore, the present study results demonstrate that the Nucleospin Plant II extraction kit is the best because it produces the purest, amplifiable, and sequenceable DNA for identification and authentication of E. Longifolia HMPs. John Wiley & Sons, Inc 2021-02 Article PeerReviewed application/pdf en http://eprints.utm.my/id/eprint/97181/1/FaezahMdSalleh2021_ComparativeEvaluationofDifferentDNA.pdf Abubakar, Bashir Mohammed and Md. Salleh, Faezah and Wagiran, Alina and Abba, Mustapha (2021) Comparative evaluation of different DNA extraction methods from E. Longifolia herbal medicinal product. eFood, 2 (1). pp. 21-26. ISSN 2666-3066 http://dx.doi.org/10.2991/efood.k.210202.001 DOI:10.2991/efood.k.210202.001
institution Universiti Teknologi Malaysia
building UTM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Malaysia
content_source UTM Institutional Repository
url_provider http://eprints.utm.my/
language English
topic Q Science (General)
spellingShingle Q Science (General)
Abubakar, Bashir Mohammed
Md. Salleh, Faezah
Wagiran, Alina
Abba, Mustapha
Comparative evaluation of different DNA extraction methods from E. Longifolia herbal medicinal product
description The aphrodisiac property of Eurycoma longifolia has led to an increase in the demand for its Herbal Medicinal Products (HMPs). However, the efficiency of such HMPs depends on the usage of their genuine raw materials. The conventional methods cannot identify species in processed form. The authentication of HMPs can be achieved effectively using DNA barcoding as the method species-specific. However, the use of this method solely relied on the extraction of high-quality DNA from the HMPs. Therefore, it is necessary to establish a satisfactory method for extracting high-quality DNA from the HMPs. Here, four DNA extraction methods were compared to evaluate the best protocol in yield, purity, polymerase chain reaction (PCR) amplification, sequencing, and species identification. The spectrophotometer analysis showed that the Nucleospin Plant II extraction kit has the best purity as this can be severely affected by the presence of various contaminants in the HMPs. Our findings reveal that DNA purity was more important as a predictor for PCR amplification than yield. Therefore, the present study results demonstrate that the Nucleospin Plant II extraction kit is the best because it produces the purest, amplifiable, and sequenceable DNA for identification and authentication of E. Longifolia HMPs.
format Article
author Abubakar, Bashir Mohammed
Md. Salleh, Faezah
Wagiran, Alina
Abba, Mustapha
author_facet Abubakar, Bashir Mohammed
Md. Salleh, Faezah
Wagiran, Alina
Abba, Mustapha
author_sort Abubakar, Bashir Mohammed
title Comparative evaluation of different DNA extraction methods from E. Longifolia herbal medicinal product
title_short Comparative evaluation of different DNA extraction methods from E. Longifolia herbal medicinal product
title_full Comparative evaluation of different DNA extraction methods from E. Longifolia herbal medicinal product
title_fullStr Comparative evaluation of different DNA extraction methods from E. Longifolia herbal medicinal product
title_full_unstemmed Comparative evaluation of different DNA extraction methods from E. Longifolia herbal medicinal product
title_sort comparative evaluation of different dna extraction methods from e. longifolia herbal medicinal product
publisher John Wiley & Sons, Inc
publishDate 2021
url http://eprints.utm.my/id/eprint/97181/1/FaezahMdSalleh2021_ComparativeEvaluationofDifferentDNA.pdf
http://eprints.utm.my/id/eprint/97181/
http://dx.doi.org/10.2991/efood.k.210202.001
_version_ 1745562348010078208
score 13.211869

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