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Cytotoxicity assay of biosynthesis gold nanoparticles mediated by walnut (Juglans regia) green husk extract

The unique properties of gold nanoparticles (Au-NPs) produce in plant extract make them attractive for use in medical and industrial applications, it is necessary to develop environmentally friendly methods for their synthesis. This can be accomplished by replacing the traditional chemical compounds...

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Main Authors:	Izadiyan, Zahra, Shameli, Kamyar, Hara, Hirofumi, Mohd. Taib, Siti Husnaa
Format:	Article
Published:	Elsevier B.V. 2018
Subjects:	Q Science (General) TP Chemical technology
Online Access:	http://eprints.utm.my/id/eprint/97093/ http://dx.doi.org/10.1016/j.molstruc.2017.09.039
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spelling	my.utm.970932022-09-12T08:07:23Z http://eprints.utm.my/id/eprint/97093/ Cytotoxicity assay of biosynthesis gold nanoparticles mediated by walnut (Juglans regia) green husk extract Izadiyan, Zahra Shameli, Kamyar Hara, Hirofumi Mohd. Taib, Siti Husnaa Q Science (General) TP Chemical technology The unique properties of gold nanoparticles (Au-NPs) produce in plant extract make them attractive for use in medical and industrial applications, it is necessary to develop environmentally friendly methods for their synthesis. This can be accomplished by replacing the traditional chemical compounds for the reduction of the gold ions to Au-NPs during synthesis with natural plant extracts or with plasmas atmospheric pressure. Here, the biosynthesis of Au-NPs using the Juglans regia (J. regia) green husk extract was investigated as the reducing and stabilizing agent. The formation of Au-NPs was initially monitored by visual observation and then characterized with the help of various characterization techniques. UV–vis spectroscopy results showed that Au-NPs synthesized using moderate temperature have a blue shifting, good distribution and smaller size compare with Au-NPs fabricated in room temperature. X-ray diffraction (XRD) results revealed the distinctive formation of the crystalline structure of Au-NPs with a spherical shape. According to transmission electron microscopy (TEM), the mean diameter and standard deviation of Au-NPs at room and moderate temperatures were 19.19 ± 4.7 and 14.32 ± 3.24 nm, respectively. The result of Field emission scanning electron microscopy (FESEM) and energy dispersive X-ray (EDX) are in good agreement with each other and confirm that by using the moderate temperature compare to the room temperature the yield of reaction increased. Based on the zeta potential result, Au-NPs has sufficient value for the stability of the solution. According to FTIR spectrum, the J. regia would be coated on the gold ions surface in a successful manner. The non-toxic effect of Au-NPs concentration below 250 μg/ml was observed in the studies of in vitro cytotoxicity on normal and cancerous cell lines, respectively. The dose-dependent toxicity made it a suitable candidate for various medical applications. Elsevier B.V. 2018-01-05 Article PeerReviewed Izadiyan, Zahra and Shameli, Kamyar and Hara, Hirofumi and Mohd. Taib, Siti Husnaa (2018) Cytotoxicity assay of biosynthesis gold nanoparticles mediated by walnut (Juglans regia) green husk extract. Journal of Molecular Structure, 1151 (NA). pp. 97-105. ISSN 0022-2860 http://dx.doi.org/10.1016/j.molstruc.2017.09.039 DOI:10.1016/j.molstruc.2017.09.039
institution	Universiti Teknologi Malaysia
building	UTM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Teknologi Malaysia
content_source	UTM Institutional Repository
url_provider	http://eprints.utm.my/
topic	Q Science (General) TP Chemical technology
spellingShingle	Q Science (General) TP Chemical technology Izadiyan, Zahra Shameli, Kamyar Hara, Hirofumi Mohd. Taib, Siti Husnaa Cytotoxicity assay of biosynthesis gold nanoparticles mediated by walnut (Juglans regia) green husk extract
description	The unique properties of gold nanoparticles (Au-NPs) produce in plant extract make them attractive for use in medical and industrial applications, it is necessary to develop environmentally friendly methods for their synthesis. This can be accomplished by replacing the traditional chemical compounds for the reduction of the gold ions to Au-NPs during synthesis with natural plant extracts or with plasmas atmospheric pressure. Here, the biosynthesis of Au-NPs using the Juglans regia (J. regia) green husk extract was investigated as the reducing and stabilizing agent. The formation of Au-NPs was initially monitored by visual observation and then characterized with the help of various characterization techniques. UV–vis spectroscopy results showed that Au-NPs synthesized using moderate temperature have a blue shifting, good distribution and smaller size compare with Au-NPs fabricated in room temperature. X-ray diffraction (XRD) results revealed the distinctive formation of the crystalline structure of Au-NPs with a spherical shape. According to transmission electron microscopy (TEM), the mean diameter and standard deviation of Au-NPs at room and moderate temperatures were 19.19 ± 4.7 and 14.32 ± 3.24 nm, respectively. The result of Field emission scanning electron microscopy (FESEM) and energy dispersive X-ray (EDX) are in good agreement with each other and confirm that by using the moderate temperature compare to the room temperature the yield of reaction increased. Based on the zeta potential result, Au-NPs has sufficient value for the stability of the solution. According to FTIR spectrum, the J. regia would be coated on the gold ions surface in a successful manner. The non-toxic effect of Au-NPs concentration below 250 μg/ml was observed in the studies of in vitro cytotoxicity on normal and cancerous cell lines, respectively. The dose-dependent toxicity made it a suitable candidate for various medical applications.
format	Article
author	Izadiyan, Zahra Shameli, Kamyar Hara, Hirofumi Mohd. Taib, Siti Husnaa
author_facet	Izadiyan, Zahra Shameli, Kamyar Hara, Hirofumi Mohd. Taib, Siti Husnaa
author_sort	Izadiyan, Zahra
title	Cytotoxicity assay of biosynthesis gold nanoparticles mediated by walnut (Juglans regia) green husk extract
title_short	Cytotoxicity assay of biosynthesis gold nanoparticles mediated by walnut (Juglans regia) green husk extract
title_full	Cytotoxicity assay of biosynthesis gold nanoparticles mediated by walnut (Juglans regia) green husk extract
title_fullStr	Cytotoxicity assay of biosynthesis gold nanoparticles mediated by walnut (Juglans regia) green husk extract
title_full_unstemmed	Cytotoxicity assay of biosynthesis gold nanoparticles mediated by walnut (Juglans regia) green husk extract
title_sort	cytotoxicity assay of biosynthesis gold nanoparticles mediated by walnut (juglans regia) green husk extract
publisher	Elsevier B.V.
publishDate	2018
url	http://eprints.utm.my/id/eprint/97093/ http://dx.doi.org/10.1016/j.molstruc.2017.09.039
_version_	1744353712699080704
score	13.211869

Cytotoxicity assay of biosynthesis gold nanoparticles mediated by walnut (Juglans regia) green husk extract

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