Staff View: Mining the genome of bacillus velezensis VB7 (CP047587) for MAMP genes and non-ribosomal peptide synthetase gene clusters conferring antiviral and antifungal activity

Mining the genome of bacillus velezensis VB7 (CP047587) for MAMP genes and non-ribosomal peptide synthetase gene clusters conferring antiviral and antifungal activity

Chemical pesticides have an immense role in curbing the infection of plant viruses and soil-borne pathogens of high valued crops. However, the usage of chemical pesticides also contributes to the development of resistance among pathogens. Hence, attempts were made in this study to identify a suitabl...

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Main Authors:	R., Saravanan, Nakkeeran, S., Saranya, N., Senthilraja, C., Renukadevi, P., Krishnamoorthy, A. S., El Enshasy, Hesham Ali, El-Adawi, Hala, Malathi, V. G., Salmen, Saleh H., Ansari, M. J., Khan, Naeem, Sayyed, R. Z.
Format:	Article
Language:	English
Published:	MDPI 2021
Subjects:	Q Science (General) TP Chemical technology
Online Access:	http://eprints.utm.my/id/eprint/95699/1/HeshamAli2021_MiningtheGenomeofBacillusVelezensis.pdf http://eprints.utm.my/id/eprint/95699/ http://dx.doi.org/10.3390/microorganisms9122511
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spelling	my.utm.956992022-05-31T13:20:41Z http://eprints.utm.my/id/eprint/95699/ Mining the genome of bacillus velezensis VB7 (CP047587) for MAMP genes and non-ribosomal peptide synthetase gene clusters conferring antiviral and antifungal activity R., Saravanan Nakkeeran, S. Saranya, N. Senthilraja, C. Renukadevi, P. Krishnamoorthy, A. S. El Enshasy, Hesham Ali El-Adawi, Hala Malathi, V. G. Salmen, Saleh H. Ansari, M. J. Khan, Naeem Sayyed, R. Z. Q Science (General) TP Chemical technology Chemical pesticides have an immense role in curbing the infection of plant viruses and soil-borne pathogens of high valued crops. However, the usage of chemical pesticides also contributes to the development of resistance among pathogens. Hence, attempts were made in this study to identify a suitable bacterial antagonist for managing viral and fungal pathogens infecting crop plants. Based on our earlier investigations, we identified Bacillus amyloliquefaciens VB7 as a potential antagonist for managing Sclerotinia sclerotiorum infecting carnation, tobacco streak virus infecting cotton and groundnut bud necrosis infecting tomato. Considering the multifaceted action of B. amyloliquefaciens VB7, attempts were made for whole-genome sequencing to assess the antiviral activity against tomato spotted wilt virus infecting chrysanthemum and antifungal action against Fusarium oxysporum f. sp. cubense (Foc). Genome annotation of the isolate B. amyloliquefaciens VB7 was confirmed as B. velezensis VB7 with accession number CP047587. Genome analysis revealed the presence of 9,231,928 reads with an average read length of 149 bp. Assembled genome had 1 contig, with a total length of 3,021,183 bp and an average G+C content of 46.79%. The protein-coding sequences (CDS) in the genome was 3090, transfer RNA (tRNA) genes were 85 with 29 ribosomal RNA (rRNA) genes and 21 repeat regions. The genome of B. velezensis VB7 had 506 hypothetical proteins and 2584 proteins with functional assignments. VB7 genome had the presence of flagellin protein FlaA with 987 nucleotides and translation elongation factor TU (Ef-Tu) with 1191 nucleotides. The identified ORFs were 3911 with 47.22% GC content. Non ribosomal pepide synthetase cluster (NRPS) gene clusters in the genome of VB7, coded for the anti-microbial peptides surfactin, butirosin A/butirosin B, fengycin, difficidin, bacillibactin, bacilysin, and mersacidin the Ripp lanthipeptide. Antiviral action of VB7 was confirmed by suppression of local lesion formation of TSWV in the local lesion host cowpea (Co-7). Moreover, combined application of B. velezensis VB7 with phyto-antiviral principles M. Jalapa and H. cupanioides increased shoot length, shoot diameter, number of flower buds per plant, flower diameter, and fresh weight of chrysanthemum. Further, screening for antifungal action of VB7 expressed antifungal action against Foc in vitro by producing VOC/NVOC compounds, including hexadecanoic acid, linoelaidic acid, octadecanoic acid, clindamycin, formic acid, succinamide, furanone, 4H-pyran, nonanol and oleic acid, contributing to the total suppression of Foc apart from the presence of NRPS gene clusters. Thus, our study confirmed the scope for exploring B. velezensis VB7 on a commercial scale to manage tomato spotted wilt virus, groundnut bud necrosis virus, tobacco streak virus, S. sclerotiorum, and Foc causing panama wilt of banana. MDPI 2021-12 Article PeerReviewed application/pdf en http://eprints.utm.my/id/eprint/95699/1/HeshamAli2021_MiningtheGenomeofBacillusVelezensis.pdf R., Saravanan and Nakkeeran, S. and Saranya, N. and Senthilraja, C. and Renukadevi, P. and Krishnamoorthy, A. S. and El Enshasy, Hesham Ali and El-Adawi, Hala and Malathi, V. G. and Salmen, Saleh H. and Ansari, M. J. and Khan, Naeem and Sayyed, R. Z. (2021) Mining the genome of bacillus velezensis VB7 (CP047587) for MAMP genes and non-ribosomal peptide synthetase gene clusters conferring antiviral and antifungal activity. Microorganisms, 9 (12). pp. 1-18. ISSN 2076-2607 http://dx.doi.org/10.3390/microorganisms9122511 DOI:10.3390/microorganisms9122511
institution	Universiti Teknologi Malaysia
building	UTM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Teknologi Malaysia
content_source	UTM Institutional Repository
url_provider	http://eprints.utm.my/
language	English
topic	Q Science (General) TP Chemical technology
spellingShingle	Q Science (General) TP Chemical technology R., Saravanan Nakkeeran, S. Saranya, N. Senthilraja, C. Renukadevi, P. Krishnamoorthy, A. S. El Enshasy, Hesham Ali El-Adawi, Hala Malathi, V. G. Salmen, Saleh H. Ansari, M. J. Khan, Naeem Sayyed, R. Z. Mining the genome of bacillus velezensis VB7 (CP047587) for MAMP genes and non-ribosomal peptide synthetase gene clusters conferring antiviral and antifungal activity
description	Chemical pesticides have an immense role in curbing the infection of plant viruses and soil-borne pathogens of high valued crops. However, the usage of chemical pesticides also contributes to the development of resistance among pathogens. Hence, attempts were made in this study to identify a suitable bacterial antagonist for managing viral and fungal pathogens infecting crop plants. Based on our earlier investigations, we identified Bacillus amyloliquefaciens VB7 as a potential antagonist for managing Sclerotinia sclerotiorum infecting carnation, tobacco streak virus infecting cotton and groundnut bud necrosis infecting tomato. Considering the multifaceted action of B. amyloliquefaciens VB7, attempts were made for whole-genome sequencing to assess the antiviral activity against tomato spotted wilt virus infecting chrysanthemum and antifungal action against Fusarium oxysporum f. sp. cubense (Foc). Genome annotation of the isolate B. amyloliquefaciens VB7 was confirmed as B. velezensis VB7 with accession number CP047587. Genome analysis revealed the presence of 9,231,928 reads with an average read length of 149 bp. Assembled genome had 1 contig, with a total length of 3,021,183 bp and an average G+C content of 46.79%. The protein-coding sequences (CDS) in the genome was 3090, transfer RNA (tRNA) genes were 85 with 29 ribosomal RNA (rRNA) genes and 21 repeat regions. The genome of B. velezensis VB7 had 506 hypothetical proteins and 2584 proteins with functional assignments. VB7 genome had the presence of flagellin protein FlaA with 987 nucleotides and translation elongation factor TU (Ef-Tu) with 1191 nucleotides. The identified ORFs were 3911 with 47.22% GC content. Non ribosomal pepide synthetase cluster (NRPS) gene clusters in the genome of VB7, coded for the anti-microbial peptides surfactin, butirosin A/butirosin B, fengycin, difficidin, bacillibactin, bacilysin, and mersacidin the Ripp lanthipeptide. Antiviral action of VB7 was confirmed by suppression of local lesion formation of TSWV in the local lesion host cowpea (Co-7). Moreover, combined application of B. velezensis VB7 with phyto-antiviral principles M. Jalapa and H. cupanioides increased shoot length, shoot diameter, number of flower buds per plant, flower diameter, and fresh weight of chrysanthemum. Further, screening for antifungal action of VB7 expressed antifungal action against Foc in vitro by producing VOC/NVOC compounds, including hexadecanoic acid, linoelaidic acid, octadecanoic acid, clindamycin, formic acid, succinamide, furanone, 4H-pyran, nonanol and oleic acid, contributing to the total suppression of Foc apart from the presence of NRPS gene clusters. Thus, our study confirmed the scope for exploring B. velezensis VB7 on a commercial scale to manage tomato spotted wilt virus, groundnut bud necrosis virus, tobacco streak virus, S. sclerotiorum, and Foc causing panama wilt of banana.
format	Article
author	R., Saravanan Nakkeeran, S. Saranya, N. Senthilraja, C. Renukadevi, P. Krishnamoorthy, A. S. El Enshasy, Hesham Ali El-Adawi, Hala Malathi, V. G. Salmen, Saleh H. Ansari, M. J. Khan, Naeem Sayyed, R. Z.
author_facet	R., Saravanan Nakkeeran, S. Saranya, N. Senthilraja, C. Renukadevi, P. Krishnamoorthy, A. S. El Enshasy, Hesham Ali El-Adawi, Hala Malathi, V. G. Salmen, Saleh H. Ansari, M. J. Khan, Naeem Sayyed, R. Z.
author_sort	R., Saravanan
title	Mining the genome of bacillus velezensis VB7 (CP047587) for MAMP genes and non-ribosomal peptide synthetase gene clusters conferring antiviral and antifungal activity
title_short	Mining the genome of bacillus velezensis VB7 (CP047587) for MAMP genes and non-ribosomal peptide synthetase gene clusters conferring antiviral and antifungal activity
title_full	Mining the genome of bacillus velezensis VB7 (CP047587) for MAMP genes and non-ribosomal peptide synthetase gene clusters conferring antiviral and antifungal activity
title_fullStr	Mining the genome of bacillus velezensis VB7 (CP047587) for MAMP genes and non-ribosomal peptide synthetase gene clusters conferring antiviral and antifungal activity
title_full_unstemmed	Mining the genome of bacillus velezensis VB7 (CP047587) for MAMP genes and non-ribosomal peptide synthetase gene clusters conferring antiviral and antifungal activity
title_sort	mining the genome of bacillus velezensis vb7 (cp047587) for mamp genes and non-ribosomal peptide synthetase gene clusters conferring antiviral and antifungal activity
publisher	MDPI
publishDate	2021
url	http://eprints.utm.my/id/eprint/95699/1/HeshamAli2021_MiningtheGenomeofBacillusVelezensis.pdf http://eprints.utm.my/id/eprint/95699/ http://dx.doi.org/10.3390/microorganisms9122511
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score	13.211869

Mining the genome of bacillus velezensis VB7 (CP047587) for MAMP genes and non-ribosomal peptide synthetase gene clusters conferring antiviral and antifungal activity

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