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Lipase production through solid-state fermentation on coconut pulp using mold isolated from fermented soybean

Lipases (triacylglycerol acylhydrolase, E.C. 3.1.1.3 are a class of enzymes that catalyze several important reactions viz. hydrolysis, aminolysis, alcoholysis, and esterification. Hence, switching to lipase production using low-cost cultivation media through solid-state fermentation (SSF) of agro-in...

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Main Authors: Nanik Wijayati, Nanik Wijayati, Miftah Al Azizah, Miftah Al Azizah, Sri Mursiti, Sri Mursiti, Abdul Wahab, Roswanira
Format: Article
Published: University of Chemical Technology and Metallurgy 2021
Subjects:
QD Chemistry
Online Access:http://eprints.utm.my/id/eprint/95656/
https://dl.uctm.edu/journal/node/j2021-2/8_19-241p307-314.pdf
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spelling my.utm.956562022-05-31T13:04:33Z http://eprints.utm.my/id/eprint/95656/ Lipase production through solid-state fermentation on coconut pulp using mold isolated from fermented soybean Nanik Wijayati, Nanik Wijayati Miftah Al Azizah, Miftah Al Azizah Sri Mursiti, Sri Mursiti Abdul Wahab, Roswanira QD Chemistry Lipases (triacylglycerol acylhydrolase, E.C. 3.1.1.3 are a class of enzymes that catalyze several important reactions viz. hydrolysis, aminolysis, alcoholysis, and esterification. Hence, switching to lipase production using low-cost cultivation media through solid-state fermentation (SSF) of agro-industry biomass may prove beneficial in lowering the production cost. In this study, mold isolated from fermented soybean was selected for producing lipase through SSF using coconut pulp as the substrate. Results revealed that a 4-day incubation was optimum for the SSF of coconut pulp to produce the highest lipase activity and protein content, which are 12.0 U ml-1 and 0.75 mg ml-1, respectively. Specific activity of the lipase reached its maximum at 15.9 U mg-1 with incubation at 35°C and pH 7.5. Moreover, it catalyzed the hydrolysis of olive oil with an activation energy of 43.46 kJ mol-1. Results demonstrated that the strategy adopted in this study to produce lipase by SSF of coconut pulp appeared to be a promising approach. University of Chemical Technology and Metallurgy 2021 Article PeerReviewed Nanik Wijayati, Nanik Wijayati and Miftah Al Azizah, Miftah Al Azizah and Sri Mursiti, Sri Mursiti and Abdul Wahab, Roswanira (2021) Lipase production through solid-state fermentation on coconut pulp using mold isolated from fermented soybean. Journal of Chemical Technology and Metallurgy, 56 (2). pp. 307-314. ISSN 1314-7471 https://dl.uctm.edu/journal/node/j2021-2/8_19-241p307-314.pdf
institution Universiti Teknologi Malaysia
building UTM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Malaysia
content_source UTM Institutional Repository
url_provider http://eprints.utm.my/
topic QD Chemistry
spellingShingle QD Chemistry
Nanik Wijayati, Nanik Wijayati
Miftah Al Azizah, Miftah Al Azizah
Sri Mursiti, Sri Mursiti
Abdul Wahab, Roswanira
Lipase production through solid-state fermentation on coconut pulp using mold isolated from fermented soybean
description Lipases (triacylglycerol acylhydrolase, E.C. 3.1.1.3 are a class of enzymes that catalyze several important reactions viz. hydrolysis, aminolysis, alcoholysis, and esterification. Hence, switching to lipase production using low-cost cultivation media through solid-state fermentation (SSF) of agro-industry biomass may prove beneficial in lowering the production cost. In this study, mold isolated from fermented soybean was selected for producing lipase through SSF using coconut pulp as the substrate. Results revealed that a 4-day incubation was optimum for the SSF of coconut pulp to produce the highest lipase activity and protein content, which are 12.0 U ml-1 and 0.75 mg ml-1, respectively. Specific activity of the lipase reached its maximum at 15.9 U mg-1 with incubation at 35°C and pH 7.5. Moreover, it catalyzed the hydrolysis of olive oil with an activation energy of 43.46 kJ mol-1. Results demonstrated that the strategy adopted in this study to produce lipase by SSF of coconut pulp appeared to be a promising approach.
format Article
author Nanik Wijayati, Nanik Wijayati
Miftah Al Azizah, Miftah Al Azizah
Sri Mursiti, Sri Mursiti
Abdul Wahab, Roswanira
author_facet Nanik Wijayati, Nanik Wijayati
Miftah Al Azizah, Miftah Al Azizah
Sri Mursiti, Sri Mursiti
Abdul Wahab, Roswanira
author_sort Nanik Wijayati, Nanik Wijayati
title Lipase production through solid-state fermentation on coconut pulp using mold isolated from fermented soybean
title_short Lipase production through solid-state fermentation on coconut pulp using mold isolated from fermented soybean
title_full Lipase production through solid-state fermentation on coconut pulp using mold isolated from fermented soybean
title_fullStr Lipase production through solid-state fermentation on coconut pulp using mold isolated from fermented soybean
title_full_unstemmed Lipase production through solid-state fermentation on coconut pulp using mold isolated from fermented soybean
title_sort lipase production through solid-state fermentation on coconut pulp using mold isolated from fermented soybean
publisher University of Chemical Technology and Metallurgy
publishDate 2021
url http://eprints.utm.my/id/eprint/95656/
https://dl.uctm.edu/journal/node/j2021-2/8_19-241p307-314.pdf
_version_ 1735386831173713920
score 13.211869

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