Staff View: Integrated ultrasound-mechanical stirrer technique for extraction of total alkaloid content from annona muricata

Integrated ultrasound-mechanical stirrer technique for extraction of total alkaloid content from annona muricata

Ultrasound-mechanical stirrer assisted extraction (UMSAE) of extraction yield, total alkaloid content (TAC) and antioxidant (DPPH) activity from Annona muricata leaves were optimized using response surface methodology. Five factors were screened using Plackett-Burman Design to investigate the variab...

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Main Authors:	Lee, C. H., Lee, T. H., Ong, P. Y., Wong, W. S., Hamdan, N., Elgharbawy, A. A. M., Azmi, N. A. A.
Format:	Article
Published:	Elsevier Ltd. 2021
Subjects:	TP Chemical technology
Online Access:	http://eprints.utm.my/id/eprint/95301/ http://dx.doi.org/10.1016/j.procbio.2021.07.006
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spelling	my.utm.953012022-04-29T22:03:20Z http://eprints.utm.my/id/eprint/95301/ Integrated ultrasound-mechanical stirrer technique for extraction of total alkaloid content from annona muricata Lee, C. H. Lee, T. H. Ong, P. Y. Wong, W. S. Hamdan, N. Elgharbawy, A. A. M. Azmi, N. A. A. TP Chemical technology Ultrasound-mechanical stirrer assisted extraction (UMSAE) of extraction yield, total alkaloid content (TAC) and antioxidant (DPPH) activity from Annona muricata leaves were optimized using response surface methodology. Five factors were screened using Plackett-Burman Design to investigate the variables that significantly influence the extraction yield, TAC and DPPH activity, namely solvent concentration, extraction time, ultrasound power, plant material to solvent ratio, and extraction temperature. According to statistical significance analysis, solvent concentration, ultrasound power, plant material to solvent ratio, and extraction temperature were selected for further optimization of the UMSAE extraction process using a Central Composite Design. ANOVA results exhibited that the obtained models were significant at 95 % confidence level. Satisfactory extraction yields (26.38 %) and TAC (39.73 mg AE/g) were obtained with relatively short extraction time under the optimum conditions compared with conventional Soxhlet extraction. The extract prepared under UMSAE displayed excellent DPPH activity (93.15 %). The optimized UMSAE promoted considerable yield increases 1.59-fold the yield extract, 2.32-fold TAC, and 1.19-fold antioxidant activity if compared to the Soxhlet extraction. This study provides the ideal integrated UMSAE settings for the maximum recovery of total alkaloid content with high antioxidant activity from A. muricata for possible utilization of their leaves in food applications. Elsevier Ltd. 2021 Article PeerReviewed Lee, C. H. and Lee, T. H. and Ong, P. Y. and Wong, W. S. and Hamdan, N. and Elgharbawy, A. A. M. and Azmi, N. A. A. (2021) Integrated ultrasound-mechanical stirrer technique for extraction of total alkaloid content from annona muricata. Process Biochemistry, 109 . pp. 104-116. ISSN 1359-5113 http://dx.doi.org/10.1016/j.procbio.2021.07.006 DOI: 10.1016/j.procbio.2021.07.006
institution	Universiti Teknologi Malaysia
building	UTM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Teknologi Malaysia
content_source	UTM Institutional Repository
url_provider	http://eprints.utm.my/
topic	TP Chemical technology
spellingShingle	TP Chemical technology Lee, C. H. Lee, T. H. Ong, P. Y. Wong, W. S. Hamdan, N. Elgharbawy, A. A. M. Azmi, N. A. A. Integrated ultrasound-mechanical stirrer technique for extraction of total alkaloid content from annona muricata
description	Ultrasound-mechanical stirrer assisted extraction (UMSAE) of extraction yield, total alkaloid content (TAC) and antioxidant (DPPH) activity from Annona muricata leaves were optimized using response surface methodology. Five factors were screened using Plackett-Burman Design to investigate the variables that significantly influence the extraction yield, TAC and DPPH activity, namely solvent concentration, extraction time, ultrasound power, plant material to solvent ratio, and extraction temperature. According to statistical significance analysis, solvent concentration, ultrasound power, plant material to solvent ratio, and extraction temperature were selected for further optimization of the UMSAE extraction process using a Central Composite Design. ANOVA results exhibited that the obtained models were significant at 95 % confidence level. Satisfactory extraction yields (26.38 %) and TAC (39.73 mg AE/g) were obtained with relatively short extraction time under the optimum conditions compared with conventional Soxhlet extraction. The extract prepared under UMSAE displayed excellent DPPH activity (93.15 %). The optimized UMSAE promoted considerable yield increases 1.59-fold the yield extract, 2.32-fold TAC, and 1.19-fold antioxidant activity if compared to the Soxhlet extraction. This study provides the ideal integrated UMSAE settings for the maximum recovery of total alkaloid content with high antioxidant activity from A. muricata for possible utilization of their leaves in food applications.
format	Article
author	Lee, C. H. Lee, T. H. Ong, P. Y. Wong, W. S. Hamdan, N. Elgharbawy, A. A. M. Azmi, N. A. A.
author_facet	Lee, C. H. Lee, T. H. Ong, P. Y. Wong, W. S. Hamdan, N. Elgharbawy, A. A. M. Azmi, N. A. A.
author_sort	Lee, C. H.
title	Integrated ultrasound-mechanical stirrer technique for extraction of total alkaloid content from annona muricata
title_short	Integrated ultrasound-mechanical stirrer technique for extraction of total alkaloid content from annona muricata
title_full	Integrated ultrasound-mechanical stirrer technique for extraction of total alkaloid content from annona muricata
title_fullStr	Integrated ultrasound-mechanical stirrer technique for extraction of total alkaloid content from annona muricata
title_full_unstemmed	Integrated ultrasound-mechanical stirrer technique for extraction of total alkaloid content from annona muricata
title_sort	integrated ultrasound-mechanical stirrer technique for extraction of total alkaloid content from annona muricata
publisher	Elsevier Ltd.
publishDate	2021
url	http://eprints.utm.my/id/eprint/95301/ http://dx.doi.org/10.1016/j.procbio.2021.07.006
_version_	1732945456373170176
score	13.211869

Integrated ultrasound-mechanical stirrer technique for extraction of total alkaloid content from annona muricata

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