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Pomegranate peels waste hydrolyzate optimization by response surface methodology for bioethanol production

Unwanted agricultural waste is largely comprised of lignocellulosic substrate which could be transformed into sugars. The production of bioethanol from garbage manifested an agreeable proposal towards waste management as well as energy causation. The goal of this work is to optimize parameters for g...

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Main Authors: Chaudhary, A., Hussain, Z., Aihetasham, A., Sharnouby, M. E., Rehman, R. A., Khan, M. A. U., Zahra, S., Saleem, A., Azhar, S., Alhazmi, A., Askary, A. E., Sayed, S., Enshasy, H. A. E., Hanapi, S. Z., Qamer, S.
Format: Article
Language:English
Published: Elsevier Ltd. 2021
Subjects:
TP Chemical technology
Online Access:http://eprints.utm.my/id/eprint/94912/1/SitiZulaihaHanapi2021_PomegranatePeelsWasteHydrolyzate.pdf
http://eprints.utm.my/id/eprint/94912/
http://dx.doi.org/10.1016/j.sjbs.2021.06.081
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spelling my.utm.949122022-04-29T21:55:03Z http://eprints.utm.my/id/eprint/94912/ Pomegranate peels waste hydrolyzate optimization by response surface methodology for bioethanol production Chaudhary, A. Hussain, Z. Aihetasham, A. Sharnouby, M. E. Rehman, R. A. Khan, M. A. U. Zahra, S. Saleem, A. Azhar, S. Alhazmi, A. Askary, A. E. Sayed, S. Enshasy, H. A. E. Hanapi, S. Z. Qamer, S. TP Chemical technology Unwanted agricultural waste is largely comprised of lignocellulosic substrate which could be transformed into sugars. The production of bioethanol from garbage manifested an agreeable proposal towards waste management as well as energy causation. The goal of this work is to optimize parameters for generation of bioethanol through fermentation by different yeast strains while Saccharomyces cerevisiae used as standard strain. The low cost fermentable sugars from pomegranate peels waste (PPW) were obtained by hydrolysis with HNO3 (1 to 5%). The optimum levels of hydrolysis time and temperature were elucidated via RSM (CCD) ranging from 30 to 60 min and 50 to 100 °C respectively. The result shows that optimum values (g/L) for reducing sugars was 61.45 ± 0.01 while for total carbohydrates was 236 ± 0.01. These values were found when PPW was hydrolyzed with 3% HNO3, at 75 °C for one hour. The hydrolyzates obtained from the dilute HNO3 pretreated PPW yielded a maximum of 0.43 ± 0.04, 0.41 ± 0.03 g ethanol per g of reducing sugars by both Metchnikowia sp. Y31 and M. cibodasensis Y34 at day 7 of ethanologenic experiment. The current study exhibited that by fermentation of dilute HNO3 hydrolyzates of PPW could develop copious amount of ethanol by optimized conditions. Elsevier Ltd. 2021 Article PeerReviewed application/pdf en http://eprints.utm.my/id/eprint/94912/1/SitiZulaihaHanapi2021_PomegranatePeelsWasteHydrolyzate.pdf Chaudhary, A. and Hussain, Z. and Aihetasham, A. and Sharnouby, M. E. and Rehman, R. A. and Khan, M. A. U. and Zahra, S. and Saleem, A. and Azhar, S. and Alhazmi, A. and Askary, A. E. and Sayed, S. and Enshasy, H. A. E. and Hanapi, S. Z. and Qamer, S. (2021) Pomegranate peels waste hydrolyzate optimization by response surface methodology for bioethanol production. Saudi Journal of Biological Sciences, 28 (9). ISSN 1319-562X http://dx.doi.org/10.1016/j.sjbs.2021.06.081 DOI: 10.1016/j.sjbs.2021.06.081
institution Universiti Teknologi Malaysia
building UTM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Malaysia
content_source UTM Institutional Repository
url_provider http://eprints.utm.my/
language English
topic TP Chemical technology
spellingShingle TP Chemical technology
Chaudhary, A.
Hussain, Z.
Aihetasham, A.
Sharnouby, M. E.
Rehman, R. A.
Khan, M. A. U.
Zahra, S.
Saleem, A.
Azhar, S.
Alhazmi, A.
Askary, A. E.
Sayed, S.
Enshasy, H. A. E.
Hanapi, S. Z.
Qamer, S.
Pomegranate peels waste hydrolyzate optimization by response surface methodology for bioethanol production
description Unwanted agricultural waste is largely comprised of lignocellulosic substrate which could be transformed into sugars. The production of bioethanol from garbage manifested an agreeable proposal towards waste management as well as energy causation. The goal of this work is to optimize parameters for generation of bioethanol through fermentation by different yeast strains while Saccharomyces cerevisiae used as standard strain. The low cost fermentable sugars from pomegranate peels waste (PPW) were obtained by hydrolysis with HNO3 (1 to 5%). The optimum levels of hydrolysis time and temperature were elucidated via RSM (CCD) ranging from 30 to 60 min and 50 to 100 °C respectively. The result shows that optimum values (g/L) for reducing sugars was 61.45 ± 0.01 while for total carbohydrates was 236 ± 0.01. These values were found when PPW was hydrolyzed with 3% HNO3, at 75 °C for one hour. The hydrolyzates obtained from the dilute HNO3 pretreated PPW yielded a maximum of 0.43 ± 0.04, 0.41 ± 0.03 g ethanol per g of reducing sugars by both Metchnikowia sp. Y31 and M. cibodasensis Y34 at day 7 of ethanologenic experiment. The current study exhibited that by fermentation of dilute HNO3 hydrolyzates of PPW could develop copious amount of ethanol by optimized conditions.
format Article
author Chaudhary, A.
Hussain, Z.
Aihetasham, A.
Sharnouby, M. E.
Rehman, R. A.
Khan, M. A. U.
Zahra, S.
Saleem, A.
Azhar, S.
Alhazmi, A.
Askary, A. E.
Sayed, S.
Enshasy, H. A. E.
Hanapi, S. Z.
Qamer, S.
author_facet Chaudhary, A.
Hussain, Z.
Aihetasham, A.
Sharnouby, M. E.
Rehman, R. A.
Khan, M. A. U.
Zahra, S.
Saleem, A.
Azhar, S.
Alhazmi, A.
Askary, A. E.
Sayed, S.
Enshasy, H. A. E.
Hanapi, S. Z.
Qamer, S.
author_sort Chaudhary, A.
title Pomegranate peels waste hydrolyzate optimization by response surface methodology for bioethanol production
title_short Pomegranate peels waste hydrolyzate optimization by response surface methodology for bioethanol production
title_full Pomegranate peels waste hydrolyzate optimization by response surface methodology for bioethanol production
title_fullStr Pomegranate peels waste hydrolyzate optimization by response surface methodology for bioethanol production
title_full_unstemmed Pomegranate peels waste hydrolyzate optimization by response surface methodology for bioethanol production
title_sort pomegranate peels waste hydrolyzate optimization by response surface methodology for bioethanol production
publisher Elsevier Ltd.
publishDate 2021
url http://eprints.utm.my/id/eprint/94912/1/SitiZulaihaHanapi2021_PomegranatePeelsWasteHydrolyzate.pdf
http://eprints.utm.my/id/eprint/94912/
http://dx.doi.org/10.1016/j.sjbs.2021.06.081
_version_ 1732945409271136256
score 13.211869

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