Staff View: Role of bacillus cereus in improving the growth and phytoextractability of brassica nigra (L.) K. Koch in chromium contaminated soil

Role of bacillus cereus in improving the growth and phytoextractability of brassica nigra (L.) K. Koch in chromium contaminated soil

Plant growth-promoting rhizobacteria (PGPR) mediate heavy metal tolerance and improve phytoextraction potential in plants. The present research was conducted to find the potential of bacterial strains in improving the growth and phytoextraction abilities of Brassica nigra (L.) K. Koch. in chromium c...

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Main Authors:	Akhtar, Nosheen, Ilyas, Noshin, Yasmin, Humaira, Sayyed, R. Z., Hasnain, Zuhair, Elsayed, Elsayed A., El Enshasy, Hesham A.
Format:	Article
Language:	English
Published:	MDPI AG 2021
Subjects:	TP Chemical technology
Online Access:	http://eprints.utm.my/id/eprint/94878/1/HeshamAElEnshasy2021_RoleofBacillusCereusinImprovingtheGrowth.pdf http://eprints.utm.my/id/eprint/94878/ http://dx.doi.org/10.3390/molecules26061569
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spelling	my.utm.948782022-04-29T22:22:02Z http://eprints.utm.my/id/eprint/94878/ Role of bacillus cereus in improving the growth and phytoextractability of brassica nigra (L.) K. Koch in chromium contaminated soil Akhtar, Nosheen Ilyas, Noshin Yasmin, Humaira Sayyed, R. Z. Hasnain, Zuhair Elsayed, Elsayed A. El Enshasy, Hesham A. TP Chemical technology Plant growth-promoting rhizobacteria (PGPR) mediate heavy metal tolerance and improve phytoextraction potential in plants. The present research was conducted to find the potential of bacterial strains in improving the growth and phytoextraction abilities of Brassica nigra (L.) K. Koch. in chromium contaminated soil. In this study, a total of 15 bacterial strains were isolated from heavy metal polluted soil and were screened for their heavy metal tolerance and plant growth promotion potential. The most efficient strain was identified by 16S rRNA gene sequencing and was identified as Bacillus cereus. The isolate also showed the potential to solubilize phosphate and synthesize siderophore, phytohormones (indole acetic acid, cytokinin, and abscisic acid), and osmolyte (proline and sugar) in chromium (Cr+3 ) supplemented medium. The results of the present study showed that chromium stress has negative effects on seed germination and plant growth in B. nigra while inoculation of B. cereus improved plant growth and reduced chromium toxicity. The increase in seed germination percentage, shoot length, and root length was 28.07%, 35.86%, 19.11% while the fresh and dry biomass of the plant increased by 48.00% and 62.16%, respectively, as compared to the uninoculated/control plants. The photosynthetic pigments were also improved by bacterial inoculation as compared to untreated stress-exposed plants, i.e., increase in chlorophyll a, chlorophyll b, chlorophyll a + b, and carotenoid was d 25.94%, 10.65%, 20.35%, and 44.30%, respectively. Bacterial inoculation also resulted in osmotic adjustment (proline 8.76% and sugar 28.71%) and maintained the membrane stability (51.39%) which was also indicated by reduced malondialdehyde content (59.53% decrease). The antioxidant enzyme activities were also improved to 35.90% (superoxide dismutase), 59.61% (peroxide), and 33.33% (catalase) in inoculated stress-exposed plants as compared to the control plants. B. cereus inoculation also improved the uptake, bioaccumulation, and translocation of Cr in the plant. Data showed that B. cereus also increased Cr content in the root (2.71-fold) and shoot (4.01-fold), its bioaccumulation (2.71-fold in root and 4.03-fold in the shoot) and translocation (40%) was also high in B. nigra. The data revealed that B. cereus is a multifarious PGPR that efficiently tolerates heavy metal ions (Cr+3 ) and it can be used to enhance the growth and phytoextraction potential of B. nigra in heavy metal contaminated soil. MDPI AG 2021 Article PeerReviewed application/pdf en http://eprints.utm.my/id/eprint/94878/1/HeshamAElEnshasy2021_RoleofBacillusCereusinImprovingtheGrowth.pdf Akhtar, Nosheen and Ilyas, Noshin and Yasmin, Humaira and Sayyed, R. Z. and Hasnain, Zuhair and Elsayed, Elsayed A. and El Enshasy, Hesham A. (2021) Role of bacillus cereus in improving the growth and phytoextractability of brassica nigra (L.) K. Koch in chromium contaminated soil. Molecules, 26 (6). p. 1569. ISSN 1420-3049 http://dx.doi.org/10.3390/molecules26061569 olume , Issue Article number
institution	Universiti Teknologi Malaysia
building	UTM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Teknologi Malaysia
content_source	UTM Institutional Repository
url_provider	http://eprints.utm.my/
language	English
topic	TP Chemical technology
spellingShingle	TP Chemical technology Akhtar, Nosheen Ilyas, Noshin Yasmin, Humaira Sayyed, R. Z. Hasnain, Zuhair Elsayed, Elsayed A. El Enshasy, Hesham A. Role of bacillus cereus in improving the growth and phytoextractability of brassica nigra (L.) K. Koch in chromium contaminated soil
description	Plant growth-promoting rhizobacteria (PGPR) mediate heavy metal tolerance and improve phytoextraction potential in plants. The present research was conducted to find the potential of bacterial strains in improving the growth and phytoextraction abilities of Brassica nigra (L.) K. Koch. in chromium contaminated soil. In this study, a total of 15 bacterial strains were isolated from heavy metal polluted soil and were screened for their heavy metal tolerance and plant growth promotion potential. The most efficient strain was identified by 16S rRNA gene sequencing and was identified as Bacillus cereus. The isolate also showed the potential to solubilize phosphate and synthesize siderophore, phytohormones (indole acetic acid, cytokinin, and abscisic acid), and osmolyte (proline and sugar) in chromium (Cr+3 ) supplemented medium. The results of the present study showed that chromium stress has negative effects on seed germination and plant growth in B. nigra while inoculation of B. cereus improved plant growth and reduced chromium toxicity. The increase in seed germination percentage, shoot length, and root length was 28.07%, 35.86%, 19.11% while the fresh and dry biomass of the plant increased by 48.00% and 62.16%, respectively, as compared to the uninoculated/control plants. The photosynthetic pigments were also improved by bacterial inoculation as compared to untreated stress-exposed plants, i.e., increase in chlorophyll a, chlorophyll b, chlorophyll a + b, and carotenoid was d 25.94%, 10.65%, 20.35%, and 44.30%, respectively. Bacterial inoculation also resulted in osmotic adjustment (proline 8.76% and sugar 28.71%) and maintained the membrane stability (51.39%) which was also indicated by reduced malondialdehyde content (59.53% decrease). The antioxidant enzyme activities were also improved to 35.90% (superoxide dismutase), 59.61% (peroxide), and 33.33% (catalase) in inoculated stress-exposed plants as compared to the control plants. B. cereus inoculation also improved the uptake, bioaccumulation, and translocation of Cr in the plant. Data showed that B. cereus also increased Cr content in the root (2.71-fold) and shoot (4.01-fold), its bioaccumulation (2.71-fold in root and 4.03-fold in the shoot) and translocation (40%) was also high in B. nigra. The data revealed that B. cereus is a multifarious PGPR that efficiently tolerates heavy metal ions (Cr+3 ) and it can be used to enhance the growth and phytoextraction potential of B. nigra in heavy metal contaminated soil.
format	Article
author	Akhtar, Nosheen Ilyas, Noshin Yasmin, Humaira Sayyed, R. Z. Hasnain, Zuhair Elsayed, Elsayed A. El Enshasy, Hesham A.
author_facet	Akhtar, Nosheen Ilyas, Noshin Yasmin, Humaira Sayyed, R. Z. Hasnain, Zuhair Elsayed, Elsayed A. El Enshasy, Hesham A.
author_sort	Akhtar, Nosheen
title	Role of bacillus cereus in improving the growth and phytoextractability of brassica nigra (L.) K. Koch in chromium contaminated soil
title_short	Role of bacillus cereus in improving the growth and phytoextractability of brassica nigra (L.) K. Koch in chromium contaminated soil
title_full	Role of bacillus cereus in improving the growth and phytoextractability of brassica nigra (L.) K. Koch in chromium contaminated soil
title_fullStr	Role of bacillus cereus in improving the growth and phytoextractability of brassica nigra (L.) K. Koch in chromium contaminated soil
title_full_unstemmed	Role of bacillus cereus in improving the growth and phytoextractability of brassica nigra (L.) K. Koch in chromium contaminated soil
title_sort	role of bacillus cereus in improving the growth and phytoextractability of brassica nigra (l.) k. koch in chromium contaminated soil
publisher	MDPI AG
publishDate	2021
url	http://eprints.utm.my/id/eprint/94878/1/HeshamAElEnshasy2021_RoleofBacillusCereusinImprovingtheGrowth.pdf http://eprints.utm.my/id/eprint/94878/ http://dx.doi.org/10.3390/molecules26061569
_version_	1732945405192175616
score	13.211869

Role of bacillus cereus in improving the growth and phytoextractability of brassica nigra (L.) K. Koch in chromium contaminated soil

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