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Smart and pH-sensitive rGO/arabinoxylan/chitosan composite for wound dressing: in-vitro drug delivery, antibacterial activity, and biological activities

Carbohydrate polymers are biological macromolecules that have sparked a lot of interest in wound healing due to their outstanding antibacterial properties and sustained drug release. Arabinoxylan (ARX), Chitosan (CS), and reduced graphene oxide (rGO) sheets were combined and crosslinked using tetrae...

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Main Authors: Khan, Muhammad Umar Aslam, Haider, Sajjad, Raza, Mohsin Ali, Shah, Saqlain A., Abd. Razak, Saiful Izwan, Abdul Kadir, Mohammad Rafiq, Subhan, Fazli, Haider, Adnan
Format: Article
Published: Elsevier B.V. 2021
Subjects:
Q Science (General)
Online Access:http://eprints.utm.my/id/eprint/94819/
http://dx.doi.org/10.1016/j.ijbiomac.2021.10.033
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spelling my.utm.948192022-04-29T22:27:31Z http://eprints.utm.my/id/eprint/94819/ Smart and pH-sensitive rGO/arabinoxylan/chitosan composite for wound dressing: in-vitro drug delivery, antibacterial activity, and biological activities Khan, Muhammad Umar Aslam Haider, Sajjad Raza, Mohsin Ali Shah, Saqlain A. Abd. Razak, Saiful Izwan Abdul Kadir, Mohammad Rafiq Subhan, Fazli Haider, Adnan Q Science (General) Carbohydrate polymers are biological macromolecules that have sparked a lot of interest in wound healing due to their outstanding antibacterial properties and sustained drug release. Arabinoxylan (ARX), Chitosan (CS), and reduced graphene oxide (rGO) sheets were combined and crosslinked using tetraethyl orthosilicate (TEOS) as a crosslinker to fabricate composite hydrogels and assess their potential in wound dressing for skin wound healing. Fourier-transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), atomic force microscopy (AFM), transmission electron microscopy (TEM), and biological assays were used to evaluate the composite hydrogels. FTIR validated the effective fabrication of the composite hydrogels. The rough morphologies of the composite hydrogels were revealed by SEM and AFM (as evident from the Ra values). ATC-4 was discovered to have the roughest surface. TEM revealed strong homogeneous anchoring of the rGO to the polymer matrix. However, with higher amount of rGO agglomeration was detected. The % swelling at various pHs (1−13) revealed that the hydrogels were pH-sensitive. The controlled release profile for the antibacterial drug (Silver sulfadiazine) evaluated at various pH values (4.5, 6.8, and 7.4) in PBS solution and 37 °C using the Franz diffusion method revealed maximal drug release at pH 7.4 and 37 °C. The antibacterial efficacy of the composite hydrogels against pathogens that cause serious skin diseases varied. The MC3T3-E1 cell adhered, proliferated, and differentiated well on the composite hydrogels. MC3T3-E1 cell also illustrated excellent viability (91%) and proper cylindrical morphologies on the composite hydrogels. Hence, the composite hydrogels based on ARX, CS, and rGO are promising biomaterials for treating and caring for skin wounds. Elsevier B.V. 2021-12-01 Article PeerReviewed Khan, Muhammad Umar Aslam and Haider, Sajjad and Raza, Mohsin Ali and Shah, Saqlain A. and Abd. Razak, Saiful Izwan and Abdul Kadir, Mohammad Rafiq and Subhan, Fazli and Haider, Adnan (2021) Smart and pH-sensitive rGO/arabinoxylan/chitosan composite for wound dressing: in-vitro drug delivery, antibacterial activity, and biological activities. International Journal of Biological Macromolecules, 192 . pp. 820-831. ISSN 0141-8130 http://dx.doi.org/10.1016/j.ijbiomac.2021.10.033 DOI:10.1016/j.ijbiomac.2021.10.033
institution Universiti Teknologi Malaysia
building UTM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Malaysia
content_source UTM Institutional Repository
url_provider http://eprints.utm.my/
topic Q Science (General)
spellingShingle Q Science (General)
Khan, Muhammad Umar Aslam
Haider, Sajjad
Raza, Mohsin Ali
Shah, Saqlain A.
Abd. Razak, Saiful Izwan
Abdul Kadir, Mohammad Rafiq
Subhan, Fazli
Haider, Adnan
Smart and pH-sensitive rGO/arabinoxylan/chitosan composite for wound dressing: in-vitro drug delivery, antibacterial activity, and biological activities
description Carbohydrate polymers are biological macromolecules that have sparked a lot of interest in wound healing due to their outstanding antibacterial properties and sustained drug release. Arabinoxylan (ARX), Chitosan (CS), and reduced graphene oxide (rGO) sheets were combined and crosslinked using tetraethyl orthosilicate (TEOS) as a crosslinker to fabricate composite hydrogels and assess their potential in wound dressing for skin wound healing. Fourier-transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), atomic force microscopy (AFM), transmission electron microscopy (TEM), and biological assays were used to evaluate the composite hydrogels. FTIR validated the effective fabrication of the composite hydrogels. The rough morphologies of the composite hydrogels were revealed by SEM and AFM (as evident from the Ra values). ATC-4 was discovered to have the roughest surface. TEM revealed strong homogeneous anchoring of the rGO to the polymer matrix. However, with higher amount of rGO agglomeration was detected. The % swelling at various pHs (1−13) revealed that the hydrogels were pH-sensitive. The controlled release profile for the antibacterial drug (Silver sulfadiazine) evaluated at various pH values (4.5, 6.8, and 7.4) in PBS solution and 37 °C using the Franz diffusion method revealed maximal drug release at pH 7.4 and 37 °C. The antibacterial efficacy of the composite hydrogels against pathogens that cause serious skin diseases varied. The MC3T3-E1 cell adhered, proliferated, and differentiated well on the composite hydrogels. MC3T3-E1 cell also illustrated excellent viability (91%) and proper cylindrical morphologies on the composite hydrogels. Hence, the composite hydrogels based on ARX, CS, and rGO are promising biomaterials for treating and caring for skin wounds.
format Article
author Khan, Muhammad Umar Aslam
Haider, Sajjad
Raza, Mohsin Ali
Shah, Saqlain A.
Abd. Razak, Saiful Izwan
Abdul Kadir, Mohammad Rafiq
Subhan, Fazli
Haider, Adnan
author_facet Khan, Muhammad Umar Aslam
Haider, Sajjad
Raza, Mohsin Ali
Shah, Saqlain A.
Abd. Razak, Saiful Izwan
Abdul Kadir, Mohammad Rafiq
Subhan, Fazli
Haider, Adnan
author_sort Khan, Muhammad Umar Aslam
title Smart and pH-sensitive rGO/arabinoxylan/chitosan composite for wound dressing: in-vitro drug delivery, antibacterial activity, and biological activities
title_short Smart and pH-sensitive rGO/arabinoxylan/chitosan composite for wound dressing: in-vitro drug delivery, antibacterial activity, and biological activities
title_full Smart and pH-sensitive rGO/arabinoxylan/chitosan composite for wound dressing: in-vitro drug delivery, antibacterial activity, and biological activities
title_fullStr Smart and pH-sensitive rGO/arabinoxylan/chitosan composite for wound dressing: in-vitro drug delivery, antibacterial activity, and biological activities
title_full_unstemmed Smart and pH-sensitive rGO/arabinoxylan/chitosan composite for wound dressing: in-vitro drug delivery, antibacterial activity, and biological activities
title_sort smart and ph-sensitive rgo/arabinoxylan/chitosan composite for wound dressing: in-vitro drug delivery, antibacterial activity, and biological activities
publisher Elsevier B.V.
publishDate 2021
url http://eprints.utm.my/id/eprint/94819/
http://dx.doi.org/10.1016/j.ijbiomac.2021.10.033
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score 13.211869

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