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Evaluation of chitin as a biomarker of pathogenic fungal isolates

Chitin is a polysaccharide component of the inner cell wall of fungi that has been used to estimate fungal invasion in plant products. However, its detection in major pathogenic fungal species has not been investigated. The present study aimed to determine the chitin contents of pathogenic fungal sp...

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Main Authors: Lafi, A. S. A., Santhanam, J., Khaithir, T. M. N., Musa, N. F., Huyop, F.
Format: Article
Language:English
Published: Penerbit Universiti Kebangsaan Malaysia 2021
Subjects:
Q Science (General)
Online Access:http://eprints.utm.my/id/eprint/94529/1/AhmadShehabAhmad2021_EvaluationofChitinasaBiomarker.pdf
http://eprints.utm.my/id/eprint/94529/
http://dx.doi.org/10.17576/jsm-2021-5003-15
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spelling my.utm.945292022-03-31T14:55:26Z http://eprints.utm.my/id/eprint/94529/ Evaluation of chitin as a biomarker of pathogenic fungal isolates Lafi, A. S. A. Santhanam, J. Khaithir, T. M. N. Musa, N. F. Huyop, F. Q Science (General) Chitin is a polysaccharide component of the inner cell wall of fungi that has been used to estimate fungal invasion in plant products. However, its detection in major pathogenic fungal species has not been investigated. The present study aimed to determine the chitin contents of pathogenic fungal species in order to evaluate its diagnostic potential as a biomarker for fungal infections. High performance liquid chromatography (HPLC) was used to measure chitin content. Pure chitin was acid hydrolyzed and the fluorescence of 9-fluorenylmethylchloroformate (FMOC-CI) derivatives of glucosamine produced were measured. The chitin contents of ten pathogenic fungal isolates were determined per mycelial dry weight. They varied from 18.61 (± 0.09) to 47.12 (± 0.50) μg/mg dry mycelial weight. Candida albicans and Cryptococcus neoformans exhibited the highest and lowest levels of chitin, respectively. Based upon relative amounts of chitin produced, three groups namely: High (Candida albicans, Cryptococcus gattii, Aspergillus niger and Penicillium at 47.12, 46.98, 46.05, and 44.15 μg/mg respectively), medium (Rhizopus, Aspergillus fumigatus, Fusarium solani, and Mucor at 36.61, 36.30, 35.03, and 34.84 μg/mg, respectively), and low (Candida tropicalis and Cryptococcus neoformans at 20.78 and 18.61 μg/mg, respectively), were identified. Chitin was not detectable in bacterial isolates used as controls. The chitin detection method offers a sensitive and specific tool for the quantification of chitin in pathogenic fungal isolates. The detection of chitin may be a useful assay for the diagnosis of fungal infections in clinical samples. Penerbit Universiti Kebangsaan Malaysia 2021 Article PeerReviewed application/pdf en http://eprints.utm.my/id/eprint/94529/1/AhmadShehabAhmad2021_EvaluationofChitinasaBiomarker.pdf Lafi, A. S. A. and Santhanam, J. and Khaithir, T. M. N. and Musa, N. F. and Huyop, F. (2021) Evaluation of chitin as a biomarker of pathogenic fungal isolates. Sains Malaysiana, 50 (3). ISSN 0126-6039 http://dx.doi.org/10.17576/jsm-2021-5003-15 DOI: 10.17576/jsm-2021-5003-15
institution Universiti Teknologi Malaysia
building UTM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Malaysia
content_source UTM Institutional Repository
url_provider http://eprints.utm.my/
language English
topic Q Science (General)
spellingShingle Q Science (General)
Lafi, A. S. A.
Santhanam, J.
Khaithir, T. M. N.
Musa, N. F.
Huyop, F.
Evaluation of chitin as a biomarker of pathogenic fungal isolates
description Chitin is a polysaccharide component of the inner cell wall of fungi that has been used to estimate fungal invasion in plant products. However, its detection in major pathogenic fungal species has not been investigated. The present study aimed to determine the chitin contents of pathogenic fungal species in order to evaluate its diagnostic potential as a biomarker for fungal infections. High performance liquid chromatography (HPLC) was used to measure chitin content. Pure chitin was acid hydrolyzed and the fluorescence of 9-fluorenylmethylchloroformate (FMOC-CI) derivatives of glucosamine produced were measured. The chitin contents of ten pathogenic fungal isolates were determined per mycelial dry weight. They varied from 18.61 (± 0.09) to 47.12 (± 0.50) μg/mg dry mycelial weight. Candida albicans and Cryptococcus neoformans exhibited the highest and lowest levels of chitin, respectively. Based upon relative amounts of chitin produced, three groups namely: High (Candida albicans, Cryptococcus gattii, Aspergillus niger and Penicillium at 47.12, 46.98, 46.05, and 44.15 μg/mg respectively), medium (Rhizopus, Aspergillus fumigatus, Fusarium solani, and Mucor at 36.61, 36.30, 35.03, and 34.84 μg/mg, respectively), and low (Candida tropicalis and Cryptococcus neoformans at 20.78 and 18.61 μg/mg, respectively), were identified. Chitin was not detectable in bacterial isolates used as controls. The chitin detection method offers a sensitive and specific tool for the quantification of chitin in pathogenic fungal isolates. The detection of chitin may be a useful assay for the diagnosis of fungal infections in clinical samples.
format Article
author Lafi, A. S. A.
Santhanam, J.
Khaithir, T. M. N.
Musa, N. F.
Huyop, F.
author_facet Lafi, A. S. A.
Santhanam, J.
Khaithir, T. M. N.
Musa, N. F.
Huyop, F.
author_sort Lafi, A. S. A.
title Evaluation of chitin as a biomarker of pathogenic fungal isolates
title_short Evaluation of chitin as a biomarker of pathogenic fungal isolates
title_full Evaluation of chitin as a biomarker of pathogenic fungal isolates
title_fullStr Evaluation of chitin as a biomarker of pathogenic fungal isolates
title_full_unstemmed Evaluation of chitin as a biomarker of pathogenic fungal isolates
title_sort evaluation of chitin as a biomarker of pathogenic fungal isolates
publisher Penerbit Universiti Kebangsaan Malaysia
publishDate 2021
url http://eprints.utm.my/id/eprint/94529/1/AhmadShehabAhmad2021_EvaluationofChitinasaBiomarker.pdf
http://eprints.utm.my/id/eprint/94529/
http://dx.doi.org/10.17576/jsm-2021-5003-15
_version_ 1729703185096900608
score 13.211869

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