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Statistical based bioprocess design for improved production of amylase from halophilic bacillus sp. H7 isolated from marine water

Amylase (EC 3.2.1.1) enzyme has gained tremendous demand in various industries, including wastewater treatment, bioremediation and nano-biotechnology. This compels the availability of enzyme in greater yields that can be achieved by employing potential amylase-producing cultures and statistical opti...

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Main Authors: Bandal, J. N., Tile, V. A., Sayyed, R. Z., Jadhav, H. P., Wan Azelee, N. I., Danish, S., Datta, R.
Format: Article
Language:English
Published: MDPI AG 2021
Subjects:
TP Chemical technology
Online Access:http://eprints.utm.my/id/eprint/94421/1/NIWanAzelee2021_StatisticalBasedBioprocessDesign.pdf
http://eprints.utm.my/id/eprint/94421/
http://dx.doi.org/10.3390/molecules26102833
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spelling my.utm.944212022-03-31T14:54:47Z http://eprints.utm.my/id/eprint/94421/ Statistical based bioprocess design for improved production of amylase from halophilic bacillus sp. H7 isolated from marine water Bandal, J. N. Tile, V. A. Sayyed, R. Z. Jadhav, H. P. Wan Azelee, N. I. Danish, S. Datta, R. TP Chemical technology Amylase (EC 3.2.1.1) enzyme has gained tremendous demand in various industries, including wastewater treatment, bioremediation and nano-biotechnology. This compels the availability of enzyme in greater yields that can be achieved by employing potential amylase-producing cultures and statistical optimization. The use of Plackett–Burman design (PBD) that evaluates various medium components and having two-level factorial designs help to determine the factor and its level to increase the yield of product. In the present work, we are reporting the screening of amylase-producing marine bacterial strain identified as Bacillus sp. H7 by 16S rRNA. The use of two-stage statistical optimization, i.e., PBD and response surface methodology (RSM), using central composite design (CCD) further improved the production of amylase. A 1.31-fold increase in amylase production was evident using a 5.0 L laboratory-scale bioreactor. Statistical optimization gives the exact idea of variables that influence the production of enzymes, and hence, the statistical approach offers the best way to optimize the bioprocess. The high catalytic efficiency (kcat/Km) of amylase from Bacillus sp. H7 on soluble starch was estimated to be 13.73 mL/s/mg. MDPI AG 2021-05 Article PeerReviewed application/pdf en http://eprints.utm.my/id/eprint/94421/1/NIWanAzelee2021_StatisticalBasedBioprocessDesign.pdf Bandal, J. N. and Tile, V. A. and Sayyed, R. Z. and Jadhav, H. P. and Wan Azelee, N. I. and Danish, S. and Datta, R. (2021) Statistical based bioprocess design for improved production of amylase from halophilic bacillus sp. H7 isolated from marine water. Molecules, 26 (10). ISSN 1420-3049 http://dx.doi.org/10.3390/molecules26102833 DOI: 10.3390/molecules26102833
institution Universiti Teknologi Malaysia
building UTM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Malaysia
content_source UTM Institutional Repository
url_provider http://eprints.utm.my/
language English
topic TP Chemical technology
spellingShingle TP Chemical technology
Bandal, J. N.
Tile, V. A.
Sayyed, R. Z.
Jadhav, H. P.
Wan Azelee, N. I.
Danish, S.
Datta, R.
Statistical based bioprocess design for improved production of amylase from halophilic bacillus sp. H7 isolated from marine water
description Amylase (EC 3.2.1.1) enzyme has gained tremendous demand in various industries, including wastewater treatment, bioremediation and nano-biotechnology. This compels the availability of enzyme in greater yields that can be achieved by employing potential amylase-producing cultures and statistical optimization. The use of Plackett–Burman design (PBD) that evaluates various medium components and having two-level factorial designs help to determine the factor and its level to increase the yield of product. In the present work, we are reporting the screening of amylase-producing marine bacterial strain identified as Bacillus sp. H7 by 16S rRNA. The use of two-stage statistical optimization, i.e., PBD and response surface methodology (RSM), using central composite design (CCD) further improved the production of amylase. A 1.31-fold increase in amylase production was evident using a 5.0 L laboratory-scale bioreactor. Statistical optimization gives the exact idea of variables that influence the production of enzymes, and hence, the statistical approach offers the best way to optimize the bioprocess. The high catalytic efficiency (kcat/Km) of amylase from Bacillus sp. H7 on soluble starch was estimated to be 13.73 mL/s/mg.
format Article
author Bandal, J. N.
Tile, V. A.
Sayyed, R. Z.
Jadhav, H. P.
Wan Azelee, N. I.
Danish, S.
Datta, R.
author_facet Bandal, J. N.
Tile, V. A.
Sayyed, R. Z.
Jadhav, H. P.
Wan Azelee, N. I.
Danish, S.
Datta, R.
author_sort Bandal, J. N.
title Statistical based bioprocess design for improved production of amylase from halophilic bacillus sp. H7 isolated from marine water
title_short Statistical based bioprocess design for improved production of amylase from halophilic bacillus sp. H7 isolated from marine water
title_full Statistical based bioprocess design for improved production of amylase from halophilic bacillus sp. H7 isolated from marine water
title_fullStr Statistical based bioprocess design for improved production of amylase from halophilic bacillus sp. H7 isolated from marine water
title_full_unstemmed Statistical based bioprocess design for improved production of amylase from halophilic bacillus sp. H7 isolated from marine water
title_sort statistical based bioprocess design for improved production of amylase from halophilic bacillus sp. h7 isolated from marine water
publisher MDPI AG
publishDate 2021
url http://eprints.utm.my/id/eprint/94421/1/NIWanAzelee2021_StatisticalBasedBioprocessDesign.pdf
http://eprints.utm.my/id/eprint/94421/
http://dx.doi.org/10.3390/molecules26102833
_version_ 1729703169949171712
score 13.211869

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