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Detection of Salmonella typhimurium on artificially contaminated milk by real time PCR using STM4497 and fljB primers

Detection of food-borne bacterial pathogens was developed to overcome the limitations. The aim of this research was to develop Salmonella typhimurium detection by Real Time Polymerase Chain Reaction (RT-PCR) using two pairs of primers. The ability of primer pairs to detect S. typhimurium is seen fro...

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Main Authors: Nurjayadi, M., Efrianti, U. R., Azizah, N., Kurniadewi, F., Saamia, V., Wiranatha, M., Nastassya, L., El-Enshasy, H. Ali
Format: Conference or Workshop Item
Published: 2021
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TP Chemical technology
Online Access:http://eprints.utm.my/id/eprint/94201/
http://dx.doi.org/10.1063/5.0041892
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spelling my.utm.942012022-02-28T13:25:25Z http://eprints.utm.my/id/eprint/94201/ Detection of Salmonella typhimurium on artificially contaminated milk by real time PCR using STM4497 and fljB primers Nurjayadi, M. Efrianti, U. R. Azizah, N. Kurniadewi, F. Saamia, V. Wiranatha, M. Nastassya, L. El-Enshasy, H. Ali TP Chemical technology Detection of food-borne bacterial pathogens was developed to overcome the limitations. The aim of this research was to develop Salmonella typhimurium detection by Real Time Polymerase Chain Reaction (RT-PCR) using two pairs of primers. The ability of primer pairs to detect S. typhimurium is seen from cycle threshold or Ct. Artificially contaminated milk sample with 24 ng each microliter can be detected with fljB (flagellin gene) primers on Ct 12,933 and with STM4497 (hypothetical protein code) primers on Ct 13,665. The specificity test of both primers showed that melting temperature (Tm) of fljB was 80,5 degree Celsius, and STM449 was 81,6 degree Celsius. FljB and STM4497 primers gave an average detection limit respectively of 11,75 Colony Forming Unit (CFU) each milliliter and 6,8 CFU each milliliter. The time needed throughout the detection process of S. typhimurium with fljB and STM4497 primers is faster than conventional methods. Based on the results it can be concluded that primers fljB and STM449 S. typhimurium can be applied to detection and quantification of S. typhimurium in milk samples. 2021 Conference or Workshop Item PeerReviewed Nurjayadi, M. and Efrianti, U. R. and Azizah, N. and Kurniadewi, F. and Saamia, V. and Wiranatha, M. and Nastassya, L. and El-Enshasy, H. Ali (2021) Detection of Salmonella typhimurium on artificially contaminated milk by real time PCR using STM4497 and fljB primers. In: 2nd Science and Mathematics International Conference: Transforming Research and Education of Science and Mathematics in the Digital Age, SMIC 2020, 8 - 9 August 2020, Jakarta, Indonesia. http://dx.doi.org/10.1063/5.0041892
institution Universiti Teknologi Malaysia
building UTM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Malaysia
content_source UTM Institutional Repository
url_provider http://eprints.utm.my/
topic TP Chemical technology
spellingShingle TP Chemical technology
Nurjayadi, M.
Efrianti, U. R.
Azizah, N.
Kurniadewi, F.
Saamia, V.
Wiranatha, M.
Nastassya, L.
El-Enshasy, H. Ali
Detection of Salmonella typhimurium on artificially contaminated milk by real time PCR using STM4497 and fljB primers
description Detection of food-borne bacterial pathogens was developed to overcome the limitations. The aim of this research was to develop Salmonella typhimurium detection by Real Time Polymerase Chain Reaction (RT-PCR) using two pairs of primers. The ability of primer pairs to detect S. typhimurium is seen from cycle threshold or Ct. Artificially contaminated milk sample with 24 ng each microliter can be detected with fljB (flagellin gene) primers on Ct 12,933 and with STM4497 (hypothetical protein code) primers on Ct 13,665. The specificity test of both primers showed that melting temperature (Tm) of fljB was 80,5 degree Celsius, and STM449 was 81,6 degree Celsius. FljB and STM4497 primers gave an average detection limit respectively of 11,75 Colony Forming Unit (CFU) each milliliter and 6,8 CFU each milliliter. The time needed throughout the detection process of S. typhimurium with fljB and STM4497 primers is faster than conventional methods. Based on the results it can be concluded that primers fljB and STM449 S. typhimurium can be applied to detection and quantification of S. typhimurium in milk samples.
format Conference or Workshop Item
author Nurjayadi, M.
Efrianti, U. R.
Azizah, N.
Kurniadewi, F.
Saamia, V.
Wiranatha, M.
Nastassya, L.
El-Enshasy, H. Ali
author_facet Nurjayadi, M.
Efrianti, U. R.
Azizah, N.
Kurniadewi, F.
Saamia, V.
Wiranatha, M.
Nastassya, L.
El-Enshasy, H. Ali
author_sort Nurjayadi, M.
title Detection of Salmonella typhimurium on artificially contaminated milk by real time PCR using STM4497 and fljB primers
title_short Detection of Salmonella typhimurium on artificially contaminated milk by real time PCR using STM4497 and fljB primers
title_full Detection of Salmonella typhimurium on artificially contaminated milk by real time PCR using STM4497 and fljB primers
title_fullStr Detection of Salmonella typhimurium on artificially contaminated milk by real time PCR using STM4497 and fljB primers
title_full_unstemmed Detection of Salmonella typhimurium on artificially contaminated milk by real time PCR using STM4497 and fljB primers
title_sort detection of salmonella typhimurium on artificially contaminated milk by real time pcr using stm4497 and fljb primers
publishDate 2021
url http://eprints.utm.my/id/eprint/94201/
http://dx.doi.org/10.1063/5.0041892
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score 13.211869

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