Staff View: Purification of Salmonella Typhi Fim-C recombinant proteins with Co-NTA resins as raw materials for development of rapid typhoid fever detection kit

Purification of Salmonella Typhi Fim-C recombinant proteins with Co-NTA resins as raw materials for development of rapid typhoid fever detection kit

The Fim-C-S typhi recombinant protein is a protein developed from the surface of the Salmonella typhi bacteria. This protein has been known to cause a good immune response. The protein has the potential as a raw material for immuno chromatography-based with gold nano particles as rapid detection kit...

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Main Authors:	Nurjayadi, M., Setyo, H., Hardianto, D., Agustini, K., El-Enshasy, H. Ali
Format:	Conference or Workshop Item
Published:	2021
Subjects:	TP Chemical technology
Online Access:	http://eprints.utm.my/id/eprint/94200/ http://dx.doi.org/10.1063/5.0041890
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spelling	my.utm.942002022-02-28T13:25:24Z http://eprints.utm.my/id/eprint/94200/ Purification of Salmonella Typhi Fim-C recombinant proteins with Co-NTA resins as raw materials for development of rapid typhoid fever detection kit Nurjayadi, M. Setyo, H. Hardianto, D. Agustini, K. El-Enshasy, H. Ali TP Chemical technology The Fim-C-S typhi recombinant protein is a protein developed from the surface of the Salmonella typhi bacteria. This protein has been known to cause a good immune response. The protein has the potential as a raw material for immuno chromatography-based with gold nano particles as rapid detection kits. Various alternatives are taken to obtain pure protein with the maximum amount. Previous studies have succeeded in purifying the Fim-C-S. typhi recombinant protein with the Ni/NTA approach. This study aims to obtain information on optimum conditions for Fim-C-S. typhi recombinant protein purification with Co/NTA resin as raw material for rapid detection kit, because Co/NTA resin is also known to have good specifications for purification of recombinant proteins with Histidine tagging. There are three main stages in the purification process in this system, namely binding, washing, and elution. The binding and washing processes in this study were carried out 2, 4 and 6 times. Furthermore, the elution process was carried out at an imidazole concentration of 200 mM, 250 mM, and 300 mM. The results of the analysis using ImageJ gel analysis software on the results of the characterization using SDSPAGE showed that the purification in the binding process four and six times showed almost the same intensity of the band with size 31 KDa. The calculation of the yield of purification results showed the highest number of 42.75% in the six times washing process and imidazole concentration of 300 mM. Based on the data, it can be concluded purification of Fim-C-S. typhi recombinant proteins with Co-NTA resin give good results in the binding process four times, washing six times and 300 mM elution concentration. This purified Fim-C-S. typhi recombinant protein will then be used as an antigen in the development of a typhoid fever detection rapid kits. 2021 Conference or Workshop Item PeerReviewed Nurjayadi, M. and Setyo, H. and Hardianto, D. and Agustini, K. and El-Enshasy, H. Ali (2021) Purification of Salmonella Typhi Fim-C recombinant proteins with Co-NTA resins as raw materials for development of rapid typhoid fever detection kit. In: 2nd Science and Mathematics International Conference: Transforming Research and Education of Science and Mathematics in the Digital Age, SMIC 2020, 8 - 9 August 2020, Jakarta, Indonesia. http://dx.doi.org/10.1063/5.0041890
institution	Universiti Teknologi Malaysia
building	UTM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Teknologi Malaysia
content_source	UTM Institutional Repository
url_provider	http://eprints.utm.my/
topic	TP Chemical technology
spellingShingle	TP Chemical technology Nurjayadi, M. Setyo, H. Hardianto, D. Agustini, K. El-Enshasy, H. Ali Purification of Salmonella Typhi Fim-C recombinant proteins with Co-NTA resins as raw materials for development of rapid typhoid fever detection kit
description	The Fim-C-S typhi recombinant protein is a protein developed from the surface of the Salmonella typhi bacteria. This protein has been known to cause a good immune response. The protein has the potential as a raw material for immuno chromatography-based with gold nano particles as rapid detection kits. Various alternatives are taken to obtain pure protein with the maximum amount. Previous studies have succeeded in purifying the Fim-C-S. typhi recombinant protein with the Ni/NTA approach. This study aims to obtain information on optimum conditions for Fim-C-S. typhi recombinant protein purification with Co/NTA resin as raw material for rapid detection kit, because Co/NTA resin is also known to have good specifications for purification of recombinant proteins with Histidine tagging. There are three main stages in the purification process in this system, namely binding, washing, and elution. The binding and washing processes in this study were carried out 2, 4 and 6 times. Furthermore, the elution process was carried out at an imidazole concentration of 200 mM, 250 mM, and 300 mM. The results of the analysis using ImageJ gel analysis software on the results of the characterization using SDSPAGE showed that the purification in the binding process four and six times showed almost the same intensity of the band with size 31 KDa. The calculation of the yield of purification results showed the highest number of 42.75% in the six times washing process and imidazole concentration of 300 mM. Based on the data, it can be concluded purification of Fim-C-S. typhi recombinant proteins with Co-NTA resin give good results in the binding process four times, washing six times and 300 mM elution concentration. This purified Fim-C-S. typhi recombinant protein will then be used as an antigen in the development of a typhoid fever detection rapid kits.
format	Conference or Workshop Item
author	Nurjayadi, M. Setyo, H. Hardianto, D. Agustini, K. El-Enshasy, H. Ali
author_facet	Nurjayadi, M. Setyo, H. Hardianto, D. Agustini, K. El-Enshasy, H. Ali
author_sort	Nurjayadi, M.
title	Purification of Salmonella Typhi Fim-C recombinant proteins with Co-NTA resins as raw materials for development of rapid typhoid fever detection kit
title_short	Purification of Salmonella Typhi Fim-C recombinant proteins with Co-NTA resins as raw materials for development of rapid typhoid fever detection kit
title_full	Purification of Salmonella Typhi Fim-C recombinant proteins with Co-NTA resins as raw materials for development of rapid typhoid fever detection kit
title_fullStr	Purification of Salmonella Typhi Fim-C recombinant proteins with Co-NTA resins as raw materials for development of rapid typhoid fever detection kit
title_full_unstemmed	Purification of Salmonella Typhi Fim-C recombinant proteins with Co-NTA resins as raw materials for development of rapid typhoid fever detection kit
title_sort	purification of salmonella typhi fim-c recombinant proteins with co-nta resins as raw materials for development of rapid typhoid fever detection kit
publishDate	2021
url	http://eprints.utm.my/id/eprint/94200/ http://dx.doi.org/10.1063/5.0041890
_version_	1726791496282144768
score	13.211869

Purification of Salmonella Typhi Fim-C recombinant proteins with Co-NTA resins as raw materials for development of rapid typhoid fever detection kit

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