High-titer bio-succinic acid production from sequential alkalic and metal salt pretreated empty fruit bunch via simultaneous saccharification and fermentation
Bio-succinic acid is produced from inorganic salt pretreated oil palm empty fruit bunch (OPEFB) for the first time through simultaneous saccharification and fermentation (SSF). OPEFB is an agriculture waste that can be converted into succinic acid (SA) which is a high value added product sought afte...
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my.utm.940722022-02-28T13:17:14Z http://eprints.utm.my/id/eprint/94072/ High-titer bio-succinic acid production from sequential alkalic and metal salt pretreated empty fruit bunch via simultaneous saccharification and fermentation Khairil Anwar, N. A. K. Hassan, N. Mohd. Yusof, N. Idris, A. TP Chemical technology Bio-succinic acid is produced from inorganic salt pretreated oil palm empty fruit bunch (OPEFB) for the first time through simultaneous saccharification and fermentation (SSF). OPEFB is an agriculture waste that can be converted into succinic acid (SA) which is a high value added product sought after in the chemical industry. In this study OPEFB is pretreated using sequential inorganic salt (sodium phosphate dodecahydrate and zinc chloride) before undergoing SSF using Actinobacillus succinogenes ATCC 55618. Parameters that influenced the SA production such as substrate loading, inoculum size and enzyme loading are investigated using design of experiments. The results revealed that biomass loading and enzyme loading have significant impact in SA yield. Under condition of 100 g/L biomass, 5% inoculum and cellulase enzyme 40 FPU/g OPEFB, maximum concentration of SA (65.2 g/L) and yield (0.65 g/g OPEFB) are obtained. Subsequently the model developed for predicting SA production has been successfully validated. Elsevier B.V. 2021-08 Article PeerReviewed Khairil Anwar, N. A. K. and Hassan, N. and Mohd. Yusof, N. and Idris, A. (2021) High-titer bio-succinic acid production from sequential alkalic and metal salt pretreated empty fruit bunch via simultaneous saccharification and fermentation. Industrial Crops and Products, 166 . ISSN 0926-6690 http://dx.doi.org/10.1016/j.indcrop.2021.113478 DOI: 10.1016/j.indcrop.2021.113478 |
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TP Chemical technology Khairil Anwar, N. A. K. Hassan, N. Mohd. Yusof, N. Idris, A. High-titer bio-succinic acid production from sequential alkalic and metal salt pretreated empty fruit bunch via simultaneous saccharification and fermentation |
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Bio-succinic acid is produced from inorganic salt pretreated oil palm empty fruit bunch (OPEFB) for the first time through simultaneous saccharification and fermentation (SSF). OPEFB is an agriculture waste that can be converted into succinic acid (SA) which is a high value added product sought after in the chemical industry. In this study OPEFB is pretreated using sequential inorganic salt (sodium phosphate dodecahydrate and zinc chloride) before undergoing SSF using Actinobacillus succinogenes ATCC 55618. Parameters that influenced the SA production such as substrate loading, inoculum size and enzyme loading are investigated using design of experiments. The results revealed that biomass loading and enzyme loading have significant impact in SA yield. Under condition of 100 g/L biomass, 5% inoculum and cellulase enzyme 40 FPU/g OPEFB, maximum concentration of SA (65.2 g/L) and yield (0.65 g/g OPEFB) are obtained. Subsequently the model developed for predicting SA production has been successfully validated. |
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Article |
author |
Khairil Anwar, N. A. K. Hassan, N. Mohd. Yusof, N. Idris, A. |
author_facet |
Khairil Anwar, N. A. K. Hassan, N. Mohd. Yusof, N. Idris, A. |
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Khairil Anwar, N. A. K. |
title |
High-titer bio-succinic acid production from sequential alkalic and metal salt pretreated empty fruit bunch via simultaneous saccharification and fermentation |
title_short |
High-titer bio-succinic acid production from sequential alkalic and metal salt pretreated empty fruit bunch via simultaneous saccharification and fermentation |
title_full |
High-titer bio-succinic acid production from sequential alkalic and metal salt pretreated empty fruit bunch via simultaneous saccharification and fermentation |
title_fullStr |
High-titer bio-succinic acid production from sequential alkalic and metal salt pretreated empty fruit bunch via simultaneous saccharification and fermentation |
title_full_unstemmed |
High-titer bio-succinic acid production from sequential alkalic and metal salt pretreated empty fruit bunch via simultaneous saccharification and fermentation |
title_sort |
high-titer bio-succinic acid production from sequential alkalic and metal salt pretreated empty fruit bunch via simultaneous saccharification and fermentation |
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Elsevier B.V. |
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2021 |
url |
http://eprints.utm.my/id/eprint/94072/ http://dx.doi.org/10.1016/j.indcrop.2021.113478 |
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13.211869 |