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Development of polymeric nanocomposite (xyloglucan-co-methacrylic acid/hydroxyapatite/sio2) scaffold for bone tissue engineering application in-vitro antibacterial, cytotoxicity and cell culture evaluation

Advancement and innovation in bone regeneration, specifically polymeric composite scaffolds, are of high significance for the treatment of bone defects. Xyloglucan (XG) is a polysaccharide biopolymer having a wide variety of regenerative tissue therapeutic applications due to its biocompatibility, i...

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Main Authors: Aslam Khan, M. U., Mehboob, H., Abd. Razak, S. I., Yahya, M. Y., Mohd. Yusof, A. H., Ramlee, M. H., Anand, T. J. S., Hassan, R., Aziz, A., Amin, R.
Format: Article
Published: MDPI AG 2020
Subjects:
Q Science (General)
Online Access:http://eprints.utm.my/id/eprint/93719/
http://dx.doi.org/10.3390/polym12061238
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spelling my.utm.937192021-12-31T08:40:22Z http://eprints.utm.my/id/eprint/93719/ Development of polymeric nanocomposite (xyloglucan-co-methacrylic acid/hydroxyapatite/sio2) scaffold for bone tissue engineering application in-vitro antibacterial, cytotoxicity and cell culture evaluation Aslam Khan, M. U. Mehboob, H. Abd. Razak, S. I. Yahya, M. Y. Mohd. Yusof, A. H. Ramlee, M. H. Anand, T. J. S. Hassan, R. Aziz, A. Amin, R. Q Science (General) Advancement and innovation in bone regeneration, specifically polymeric composite scaffolds, are of high significance for the treatment of bone defects. Xyloglucan (XG) is a polysaccharide biopolymer having a wide variety of regenerative tissue therapeutic applications due to its biocompatibility, in-vitro degradation and cytocompatibility. Current research is focused on the fabrication of polymeric bioactive scaffolds by freeze drying method for nanocomposite materials. The nanocomposite materials have been synthesized from free radical polymerization using n-SiO2 and n-HAp XG and Methacrylic acid (MAAc). Functional group analysis, crystallinity and surface morphology were investigated by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction analysis (XRD) and scanning electron microscopy (SEM) techniques, respectively. These bioactive polymeric scaffolds presented interconnected and well-organized porous morphology, controlled precisely by substantial ratios of n-SiO2. The swelling analysis was also performed in different media at varying temperatures (27, 37 and 47 °C) and the mechanical behavior of the dried scaffolds is also investigated. Antibacterial activities of these scaffolds were conducted against pathogenic gram-positive and gram-negative bacteria. Besides, the biological behavior of these scaffolds was evaluated by the Neutral Red dye assay against the MC3T3-E1 cell line. The scaffolds showed interesting properties for bone tissue engineering, including porosity with substantial mechanical strength, biodegradability, biocompatibility and cytocompatibility behavior. The reported polymeric bioactive scaffolds can be aspirant biomaterials for bone tissue engineering to regenerate defecated bone. MDPI AG 2020 Article PeerReviewed Aslam Khan, M. U. and Mehboob, H. and Abd. Razak, S. I. and Yahya, M. Y. and Mohd. Yusof, A. H. and Ramlee, M. H. and Anand, T. J. S. and Hassan, R. and Aziz, A. and Amin, R. (2020) Development of polymeric nanocomposite (xyloglucan-co-methacrylic acid/hydroxyapatite/sio2) scaffold for bone tissue engineering application in-vitro antibacterial, cytotoxicity and cell culture evaluation. Polymers, 12 (6). ISSN 2073-4360 http://dx.doi.org/10.3390/polym12061238 DOI: 10.3390/polym12061238
institution Universiti Teknologi Malaysia
building UTM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Malaysia
content_source UTM Institutional Repository
url_provider http://eprints.utm.my/
topic Q Science (General)
spellingShingle Q Science (General)
Aslam Khan, M. U.
Mehboob, H.
Abd. Razak, S. I.
Yahya, M. Y.
Mohd. Yusof, A. H.
Ramlee, M. H.
Anand, T. J. S.
Hassan, R.
Aziz, A.
Amin, R.
Development of polymeric nanocomposite (xyloglucan-co-methacrylic acid/hydroxyapatite/sio2) scaffold for bone tissue engineering application in-vitro antibacterial, cytotoxicity and cell culture evaluation
description Advancement and innovation in bone regeneration, specifically polymeric composite scaffolds, are of high significance for the treatment of bone defects. Xyloglucan (XG) is a polysaccharide biopolymer having a wide variety of regenerative tissue therapeutic applications due to its biocompatibility, in-vitro degradation and cytocompatibility. Current research is focused on the fabrication of polymeric bioactive scaffolds by freeze drying method for nanocomposite materials. The nanocomposite materials have been synthesized from free radical polymerization using n-SiO2 and n-HAp XG and Methacrylic acid (MAAc). Functional group analysis, crystallinity and surface morphology were investigated by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction analysis (XRD) and scanning electron microscopy (SEM) techniques, respectively. These bioactive polymeric scaffolds presented interconnected and well-organized porous morphology, controlled precisely by substantial ratios of n-SiO2. The swelling analysis was also performed in different media at varying temperatures (27, 37 and 47 °C) and the mechanical behavior of the dried scaffolds is also investigated. Antibacterial activities of these scaffolds were conducted against pathogenic gram-positive and gram-negative bacteria. Besides, the biological behavior of these scaffolds was evaluated by the Neutral Red dye assay against the MC3T3-E1 cell line. The scaffolds showed interesting properties for bone tissue engineering, including porosity with substantial mechanical strength, biodegradability, biocompatibility and cytocompatibility behavior. The reported polymeric bioactive scaffolds can be aspirant biomaterials for bone tissue engineering to regenerate defecated bone.
format Article
author Aslam Khan, M. U.
Mehboob, H.
Abd. Razak, S. I.
Yahya, M. Y.
Mohd. Yusof, A. H.
Ramlee, M. H.
Anand, T. J. S.
Hassan, R.
Aziz, A.
Amin, R.
author_facet Aslam Khan, M. U.
Mehboob, H.
Abd. Razak, S. I.
Yahya, M. Y.
Mohd. Yusof, A. H.
Ramlee, M. H.
Anand, T. J. S.
Hassan, R.
Aziz, A.
Amin, R.
author_sort Aslam Khan, M. U.
title Development of polymeric nanocomposite (xyloglucan-co-methacrylic acid/hydroxyapatite/sio2) scaffold for bone tissue engineering application in-vitro antibacterial, cytotoxicity and cell culture evaluation
title_short Development of polymeric nanocomposite (xyloglucan-co-methacrylic acid/hydroxyapatite/sio2) scaffold for bone tissue engineering application in-vitro antibacterial, cytotoxicity and cell culture evaluation
title_full Development of polymeric nanocomposite (xyloglucan-co-methacrylic acid/hydroxyapatite/sio2) scaffold for bone tissue engineering application in-vitro antibacterial, cytotoxicity and cell culture evaluation
title_fullStr Development of polymeric nanocomposite (xyloglucan-co-methacrylic acid/hydroxyapatite/sio2) scaffold for bone tissue engineering application in-vitro antibacterial, cytotoxicity and cell culture evaluation
title_full_unstemmed Development of polymeric nanocomposite (xyloglucan-co-methacrylic acid/hydroxyapatite/sio2) scaffold for bone tissue engineering application in-vitro antibacterial, cytotoxicity and cell culture evaluation
title_sort development of polymeric nanocomposite (xyloglucan-co-methacrylic acid/hydroxyapatite/sio2) scaffold for bone tissue engineering application in-vitro antibacterial, cytotoxicity and cell culture evaluation
publisher MDPI AG
publishDate 2020
url http://eprints.utm.my/id/eprint/93719/
http://dx.doi.org/10.3390/polym12061238
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score 13.211869

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