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Green synthesized montmorillonite/carrageenan/Fe3O4 nanocomposites for pH-responsive release of protocatechuic acid and its anticancer activity

Discovery of a novel anticancer drug delivery agent is important to replace conventional cancer therapies which are often accompanied by undesired side effects. This study demonstrated the synthesis of superparamagnetic magnetite nanocomposites (Fe3 O4-NCs) using a green method. Montmorillonite (MMT...

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Main Authors: Yen, Pin Yew, Shameli, Kamyar, Mohamad, Shaza Eva, Kar, Xin Lee, Teow, Sin Yeang
Format: Article
Language:English
Published: MDPI 2020
Subjects:
TP Chemical technology
Online Access:http://eprints.utm.my/id/eprint/92652/1/KamyarShameli2020_GreenSynthesizedMontmorilloniteCarrageenan.pdf
http://eprints.utm.my/id/eprint/92652/
http://dx.doi.org/10.3390/ijms21144851
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spelling my.utm.926522021-10-28T10:18:33Z http://eprints.utm.my/id/eprint/92652/ Green synthesized montmorillonite/carrageenan/Fe3O4 nanocomposites for pH-responsive release of protocatechuic acid and its anticancer activity Yen, Pin Yew Shameli, Kamyar Mohamad, Shaza Eva Kar, Xin Lee Teow, Sin Yeang TP Chemical technology Discovery of a novel anticancer drug delivery agent is important to replace conventional cancer therapies which are often accompanied by undesired side effects. This study demonstrated the synthesis of superparamagnetic magnetite nanocomposites (Fe3 O4-NCs) using a green method. Montmorillonite (MMT) was used as matrix support, while Fe3 O4 nanoparticles (NPs) and carrageenan (CR) were used as filler and stabilizer, respectively. The combination of these materials resulted in a novel nanocomposite (MMT/CR/Fe3 O4-NCs). A series of characterization experiments was conducted. The purity of MMT/CR/Fe3 O4-NCs was confirmed by X-ray diffraction (XRD) analysis. High resolution transmission electron microscopy (HRTEM) analysis revealed the uniform and spherical shape of Fe3 O4 NPs with an average particle size of 9.3 ± 1.2 nm. Vibrating sample magnetometer (VSM) analysis showed an Ms value of 2.16 emu/g with negligible coercivity which confirmed the superparamagnetic properties. Protocatechuic acid (PCA) was loaded onto the MMT/CR/Fe3 O4-NCs and a drug release study showed that 15% and 92% of PCA was released at pH 7.4 and 4.8, respectively. Cytotoxicity assays showed that both MMT/CR/Fe3 O4-NCs and MMT/CR/Fe3 O4-PCA effectively killed HCT116 which is a colorectal cancer cell line. Dose-dependent inhibition was seen and the killing was enhanced two-fold by the PCA-loaded NCs (IC50 –0.734 mg/mL) compared to the unloaded NCs (IC50 –1.5 mg/mL). This study highlights the potential use of MMT/CR/Fe3 O4-NCs as a biologically active pH-responsive drug delivery agent. Further investigations are warranted to delineate the mechanism of cell entry and cancer cell killing as well as to improve the therapeutic potential of MMT/CR/Fe3 O4-NCs. MDPI 2020-07-02 Article PeerReviewed application/pdf en http://eprints.utm.my/id/eprint/92652/1/KamyarShameli2020_GreenSynthesizedMontmorilloniteCarrageenan.pdf Yen, Pin Yew and Shameli, Kamyar and Mohamad, Shaza Eva and Kar, Xin Lee and Teow, Sin Yeang (2020) Green synthesized montmorillonite/carrageenan/Fe3O4 nanocomposites for pH-responsive release of protocatechuic acid and its anticancer activity. International Journal of Molecular Sciences, 21 (14). pp. 1-17. ISSN 1661-6596 http://dx.doi.org/10.3390/ijms21144851 DOI:10.3390/ijms21144851
institution Universiti Teknologi Malaysia
building UTM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Malaysia
content_source UTM Institutional Repository
url_provider http://eprints.utm.my/
language English
topic TP Chemical technology
spellingShingle TP Chemical technology
Yen, Pin Yew
Shameli, Kamyar
Mohamad, Shaza Eva
Kar, Xin Lee
Teow, Sin Yeang
Green synthesized montmorillonite/carrageenan/Fe3O4 nanocomposites for pH-responsive release of protocatechuic acid and its anticancer activity
description Discovery of a novel anticancer drug delivery agent is important to replace conventional cancer therapies which are often accompanied by undesired side effects. This study demonstrated the synthesis of superparamagnetic magnetite nanocomposites (Fe3 O4-NCs) using a green method. Montmorillonite (MMT) was used as matrix support, while Fe3 O4 nanoparticles (NPs) and carrageenan (CR) were used as filler and stabilizer, respectively. The combination of these materials resulted in a novel nanocomposite (MMT/CR/Fe3 O4-NCs). A series of characterization experiments was conducted. The purity of MMT/CR/Fe3 O4-NCs was confirmed by X-ray diffraction (XRD) analysis. High resolution transmission electron microscopy (HRTEM) analysis revealed the uniform and spherical shape of Fe3 O4 NPs with an average particle size of 9.3 ± 1.2 nm. Vibrating sample magnetometer (VSM) analysis showed an Ms value of 2.16 emu/g with negligible coercivity which confirmed the superparamagnetic properties. Protocatechuic acid (PCA) was loaded onto the MMT/CR/Fe3 O4-NCs and a drug release study showed that 15% and 92% of PCA was released at pH 7.4 and 4.8, respectively. Cytotoxicity assays showed that both MMT/CR/Fe3 O4-NCs and MMT/CR/Fe3 O4-PCA effectively killed HCT116 which is a colorectal cancer cell line. Dose-dependent inhibition was seen and the killing was enhanced two-fold by the PCA-loaded NCs (IC50 –0.734 mg/mL) compared to the unloaded NCs (IC50 –1.5 mg/mL). This study highlights the potential use of MMT/CR/Fe3 O4-NCs as a biologically active pH-responsive drug delivery agent. Further investigations are warranted to delineate the mechanism of cell entry and cancer cell killing as well as to improve the therapeutic potential of MMT/CR/Fe3 O4-NCs.
format Article
author Yen, Pin Yew
Shameli, Kamyar
Mohamad, Shaza Eva
Kar, Xin Lee
Teow, Sin Yeang
author_facet Yen, Pin Yew
Shameli, Kamyar
Mohamad, Shaza Eva
Kar, Xin Lee
Teow, Sin Yeang
author_sort Yen, Pin Yew
title Green synthesized montmorillonite/carrageenan/Fe3O4 nanocomposites for pH-responsive release of protocatechuic acid and its anticancer activity
title_short Green synthesized montmorillonite/carrageenan/Fe3O4 nanocomposites for pH-responsive release of protocatechuic acid and its anticancer activity
title_full Green synthesized montmorillonite/carrageenan/Fe3O4 nanocomposites for pH-responsive release of protocatechuic acid and its anticancer activity
title_fullStr Green synthesized montmorillonite/carrageenan/Fe3O4 nanocomposites for pH-responsive release of protocatechuic acid and its anticancer activity
title_full_unstemmed Green synthesized montmorillonite/carrageenan/Fe3O4 nanocomposites for pH-responsive release of protocatechuic acid and its anticancer activity
title_sort green synthesized montmorillonite/carrageenan/fe3o4 nanocomposites for ph-responsive release of protocatechuic acid and its anticancer activity
publisher MDPI
publishDate 2020
url http://eprints.utm.my/id/eprint/92652/1/KamyarShameli2020_GreenSynthesizedMontmorilloniteCarrageenan.pdf
http://eprints.utm.my/id/eprint/92652/
http://dx.doi.org/10.3390/ijms21144851
_version_ 1715189669707120640
score 13.211869

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