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Bee-based sensor using apis mellifera for detection of Andrographis paniculata volatile compounds

Honey bees can be trained to be sniffer bees due to the presence of high number of odorant receptors (170 odorant receptors) in their heads. This unique characteristic enables them to detect scent down to part per trillion level. In this study, localized honey bees (Apis mellifera) were trained by u...

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Main Author: Kerk, Wen Chiann
Format: Thesis
Language:English
Published: 2019
Subjects:
TP Chemical technology
Online Access:http://eprints.utm.my/id/eprint/92556/1/KerkWenChiannPSChE2019.pdf.pdf
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spelling my.utm.925562021-09-30T15:11:35Z http://eprints.utm.my/id/eprint/92556/ Bee-based sensor using apis mellifera for detection of Andrographis paniculata volatile compounds Kerk, Wen Chiann TP Chemical technology Honey bees can be trained to be sniffer bees due to the presence of high number of odorant receptors (170 odorant receptors) in their heads. This unique characteristic enables them to detect scent down to part per trillion level. In this study, localized honey bees (Apis mellifera) were trained by using the method of classical Pavlovian conditioning. The proboscis extension reflex (PER) of sniffer bees towards the target odor was observed and recorded. The phytochemical profile of Andrographis paniculata (A. paniculata) was constructed using headspace solid-phase microextraction coupled with gas chromatography integrated with mass spectrometer method. The volatile marker compounds were identified. The sniffing capacity of the sniffer bees was determined by varying the heating temperature from 50-120 °C, the weight of plant material from 20-100 mg and the percentage (20-100%) of the target herbal sample in the mixture of A. paniculata and Clinacanthus nutans (C. nutans). C. nutans is an herbal plant which is morphologically similar to A. paniculata and it also belongs to the Acanthaceae family. The efficiency, accuracy and sensitivity of sniffer bees were analyzed and validated statistically. The success rate of sniffer bees for heating temperatures was approximately 90 %. The success rate for minimum weight of plant sample, 20 mg was 50 %. The success rate percentage of target herbal sample increased when the percentage of A. paniculata was proportionally increased. Compounds such as caryophyllene, ß-elemene, 3,3-dimethylhexane, apiol, 6,10,14-trimethyl-2-pentadecanone and dihydroactinidiolide were detected in the gaseous mixture. The kinetics of volatile marker compounds released from the plant samples were studied to predict the concentrations of the volatile marker compounds for sniffer bee detection at 85 ?. Second-order and two-site kinetic models were selected because of the kinetic data of these volatile marker compounds fitted well to these models (R2 > 0.9). 2019 Thesis NonPeerReviewed application/pdf en http://eprints.utm.my/id/eprint/92556/1/KerkWenChiannPSChE2019.pdf.pdf Kerk, Wen Chiann (2019) Bee-based sensor using apis mellifera for detection of Andrographis paniculata volatile compounds. PhD thesis, Universiti Teknologi Malaysia. http://dms.library.utm.my:8080/vital/access/manager/Repository/vital:139229
institution Universiti Teknologi Malaysia
building UTM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Malaysia
content_source UTM Institutional Repository
url_provider http://eprints.utm.my/
language English
topic TP Chemical technology
spellingShingle TP Chemical technology
Kerk, Wen Chiann
Bee-based sensor using apis mellifera for detection of Andrographis paniculata volatile compounds
description Honey bees can be trained to be sniffer bees due to the presence of high number of odorant receptors (170 odorant receptors) in their heads. This unique characteristic enables them to detect scent down to part per trillion level. In this study, localized honey bees (Apis mellifera) were trained by using the method of classical Pavlovian conditioning. The proboscis extension reflex (PER) of sniffer bees towards the target odor was observed and recorded. The phytochemical profile of Andrographis paniculata (A. paniculata) was constructed using headspace solid-phase microextraction coupled with gas chromatography integrated with mass spectrometer method. The volatile marker compounds were identified. The sniffing capacity of the sniffer bees was determined by varying the heating temperature from 50-120 °C, the weight of plant material from 20-100 mg and the percentage (20-100%) of the target herbal sample in the mixture of A. paniculata and Clinacanthus nutans (C. nutans). C. nutans is an herbal plant which is morphologically similar to A. paniculata and it also belongs to the Acanthaceae family. The efficiency, accuracy and sensitivity of sniffer bees were analyzed and validated statistically. The success rate of sniffer bees for heating temperatures was approximately 90 %. The success rate for minimum weight of plant sample, 20 mg was 50 %. The success rate percentage of target herbal sample increased when the percentage of A. paniculata was proportionally increased. Compounds such as caryophyllene, ß-elemene, 3,3-dimethylhexane, apiol, 6,10,14-trimethyl-2-pentadecanone and dihydroactinidiolide were detected in the gaseous mixture. The kinetics of volatile marker compounds released from the plant samples were studied to predict the concentrations of the volatile marker compounds for sniffer bee detection at 85 ?. Second-order and two-site kinetic models were selected because of the kinetic data of these volatile marker compounds fitted well to these models (R2 > 0.9).
format Thesis
author Kerk, Wen Chiann
author_facet Kerk, Wen Chiann
author_sort Kerk, Wen Chiann
title Bee-based sensor using apis mellifera for detection of Andrographis paniculata volatile compounds
title_short Bee-based sensor using apis mellifera for detection of Andrographis paniculata volatile compounds
title_full Bee-based sensor using apis mellifera for detection of Andrographis paniculata volatile compounds
title_fullStr Bee-based sensor using apis mellifera for detection of Andrographis paniculata volatile compounds
title_full_unstemmed Bee-based sensor using apis mellifera for detection of Andrographis paniculata volatile compounds
title_sort bee-based sensor using apis mellifera for detection of andrographis paniculata volatile compounds
publishDate 2019
url http://eprints.utm.my/id/eprint/92556/1/KerkWenChiannPSChE2019.pdf.pdf
http://eprints.utm.my/id/eprint/92556/
http://dms.library.utm.my:8080/vital/access/manager/Repository/vital:139229
_version_ 1713199749207687168
score 13.211869

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