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Cytotoxicity and inhibitory effects of backhousia citriodora extract on melanin bio-synthesis of B16F1 melanoma cell

Tyrosinase inhibitors are gaining attention due to their iitriodora exmportant role in inhibiting melanin production. Tyrosinase inhibitors are widely used in the cosmetic industries. However, some of the well-known inhibitors are reported to be harmful and can cause undesirable side effects. Thus,...

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Main Author: Mohd. Jamil, Izzatul Nadia
Format: Thesis
Language:English
Published: 2020
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TP Chemical technology
Online Access:http://eprints.utm.my/id/eprint/92081/1/IzzatulNadiaMSChE2020.pdf.pdf
http://eprints.utm.my/id/eprint/92081/
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spelling my.utm.920812021-08-30T04:17:36Z http://eprints.utm.my/id/eprint/92081/ Cytotoxicity and inhibitory effects of backhousia citriodora extract on melanin bio-synthesis of B16F1 melanoma cell Mohd. Jamil, Izzatul Nadia TP Chemical technology Tyrosinase inhibitors are gaining attention due to their iitriodora exmportant role in inhibiting melanin production. Tyrosinase inhibitors are widely used in the cosmetic industries. However, some of the well-known inhibitors are reported to be harmful and can cause undesirable side effects. Thus, application of natural ingredients in formulation has garnered massive attention among consumers to promote health and safe lifestyle. Backhousia citriodora was first introduced in Malaysia by Qzen (M) Sdn. Bhd. in 2009, in the form of stem cuttings. In this study, the extract of B. citriodora was evaluated for its biological activities. 2,2-diphenyl-1-picrylhydrazyl assay of the extract exhibited good potential for further development as antioxidant agent with IC50 values of 53.94 mg/ml, compared to ascorbic acid (83.98 mg/ml) and trolox (77.59 mg/ml). In addition, the inhibitory effect of B. citriodora extract on melanogenesis was evaluated using B16F1 melanoma cell. The cytotoxicity study was carried out using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, water soluble tetrazolium salt assay and Sulforhodamine B assay. The cell viabilities treated with B. citriodora extract with concentration at 0.03125 - 0.10 % (v/v) showed no significant effect to the cell compared to the non-treated cell. This indicates that B. citriodora extract is non-cytotoxic to the cells under the experimental condition used. Melanogenesis measured in a-MSH-induced B16F1 melanoma cells revealed that by B. citriodora extract treatment at 0.0625 - 0.10 % (v/v), the secreted melanin and intracellular melanin were significantly reduced in dose dependent manner. It can be also deduced that the inhibitory effect of B. citriodora extract is stronger than kojic acid and arbutin (positive control used). Furthermore, cellular tyrosinase activity exhibited a similar profile to melanin content. These results showed that B. citriodora extract has the ability to suppress cellular melanin synthesis in B16F1 cells. 2020 Thesis NonPeerReviewed application/pdf en http://eprints.utm.my/id/eprint/92081/1/IzzatulNadiaMSChE2020.pdf.pdf Mohd. Jamil, Izzatul Nadia (2020) Cytotoxicity and inhibitory effects of backhousia citriodora extract on melanin bio-synthesis of B16F1 melanoma cell. Masters thesis, Universiti Teknologi Malaysia. http://dms.library.utm.my:8080/vital/access/manager/Repository/vital:139033
institution Universiti Teknologi Malaysia
building UTM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Malaysia
content_source UTM Institutional Repository
url_provider http://eprints.utm.my/
language English
topic TP Chemical technology
spellingShingle TP Chemical technology
Mohd. Jamil, Izzatul Nadia
Cytotoxicity and inhibitory effects of backhousia citriodora extract on melanin bio-synthesis of B16F1 melanoma cell
description Tyrosinase inhibitors are gaining attention due to their iitriodora exmportant role in inhibiting melanin production. Tyrosinase inhibitors are widely used in the cosmetic industries. However, some of the well-known inhibitors are reported to be harmful and can cause undesirable side effects. Thus, application of natural ingredients in formulation has garnered massive attention among consumers to promote health and safe lifestyle. Backhousia citriodora was first introduced in Malaysia by Qzen (M) Sdn. Bhd. in 2009, in the form of stem cuttings. In this study, the extract of B. citriodora was evaluated for its biological activities. 2,2-diphenyl-1-picrylhydrazyl assay of the extract exhibited good potential for further development as antioxidant agent with IC50 values of 53.94 mg/ml, compared to ascorbic acid (83.98 mg/ml) and trolox (77.59 mg/ml). In addition, the inhibitory effect of B. citriodora extract on melanogenesis was evaluated using B16F1 melanoma cell. The cytotoxicity study was carried out using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, water soluble tetrazolium salt assay and Sulforhodamine B assay. The cell viabilities treated with B. citriodora extract with concentration at 0.03125 - 0.10 % (v/v) showed no significant effect to the cell compared to the non-treated cell. This indicates that B. citriodora extract is non-cytotoxic to the cells under the experimental condition used. Melanogenesis measured in a-MSH-induced B16F1 melanoma cells revealed that by B. citriodora extract treatment at 0.0625 - 0.10 % (v/v), the secreted melanin and intracellular melanin were significantly reduced in dose dependent manner. It can be also deduced that the inhibitory effect of B. citriodora extract is stronger than kojic acid and arbutin (positive control used). Furthermore, cellular tyrosinase activity exhibited a similar profile to melanin content. These results showed that B. citriodora extract has the ability to suppress cellular melanin synthesis in B16F1 cells.
format Thesis
author Mohd. Jamil, Izzatul Nadia
author_facet Mohd. Jamil, Izzatul Nadia
author_sort Mohd. Jamil, Izzatul Nadia
title Cytotoxicity and inhibitory effects of backhousia citriodora extract on melanin bio-synthesis of B16F1 melanoma cell
title_short Cytotoxicity and inhibitory effects of backhousia citriodora extract on melanin bio-synthesis of B16F1 melanoma cell
title_full Cytotoxicity and inhibitory effects of backhousia citriodora extract on melanin bio-synthesis of B16F1 melanoma cell
title_fullStr Cytotoxicity and inhibitory effects of backhousia citriodora extract on melanin bio-synthesis of B16F1 melanoma cell
title_full_unstemmed Cytotoxicity and inhibitory effects of backhousia citriodora extract on melanin bio-synthesis of B16F1 melanoma cell
title_sort cytotoxicity and inhibitory effects of backhousia citriodora extract on melanin bio-synthesis of b16f1 melanoma cell
publishDate 2020
url http://eprints.utm.my/id/eprint/92081/1/IzzatulNadiaMSChE2020.pdf.pdf
http://eprints.utm.my/id/eprint/92081/
http://dms.library.utm.my:8080/vital/access/manager/Repository/vital:139033
_version_ 1709667381763112960
score 13.211869

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