Cover Image

Biochemical study of the effect of Nephrolepis biserrata pretreatment on exo-polygalacturonase production by Aspergilllus niger in solid state fermentation

The substrate plays an important role in solid state fermentation (SSF) in the production of exo-polygalacturonase (one type of the pectinase enzymes). The aim of this study was to investigate a pretreatment method on Nephrolepis biserrata leaves as a substrate for the production of exo-polygalactur...

Full description

Saved in:
Bibliographic Details
Main Authors: Pagarra, H., Rahman, R. A., Rachmawaty, Rachmawaty
Format: Article
Published: Foundation for Enviromental Protection and Research 2019
Subjects:
TP Chemical technology
Online Access:http://eprints.utm.my/id/eprint/91467/
http://www.ejobios.org/download/biochemical-study-of-the-effect-of-nephrolepis-biserrata-pretreatment-on-exo-polygalacturonase-6193.pdf.
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.utm.91467
record_format eprints
spelling my.utm.914672021-06-30T12:16:35Z http://eprints.utm.my/id/eprint/91467/ Biochemical study of the effect of Nephrolepis biserrata pretreatment on exo-polygalacturonase production by Aspergilllus niger in solid state fermentation Pagarra, H. Rahman, R. A. Rachmawaty, Rachmawaty TP Chemical technology The substrate plays an important role in solid state fermentation (SSF) in the production of exo-polygalacturonase (one type of the pectinase enzymes). The aim of this study was to investigate a pretreatment method on Nephrolepis biserrata leaves as a substrate for the production of exo-polygalacturonase. The pretreatment steps improved the decomposition of the lignocellulosic material in the structure of N. biserrata leaves as a substrate and allowed Aspergillus niger better access of the substrate to the enzymatic reaction. This optimised both exo-polygalacturonase activity and the growth of A. niger in SSF. Pretreatment was performed under alkali, acidic, autohydrolytic and control conditions. The highest exo-polygalacturonase activity was 39.16 U/g and biomass of A. niger was 0.39 mg/gdsf at 120 h was found for the autohydrolysis pretreatment, as compared to alkali pretreatment, acid pretreatment and control. Scanning electron microscope images showed a marked change in the physical appearance of treated N. biserrata leaves as compared to untreated N. biserrata leaves, especially following autohydrolysis pretreatment. Foundation for Enviromental Protection and Research 2019 Article PeerReviewed Pagarra, H. and Rahman, R. A. and Rachmawaty, Rachmawaty (2019) Biochemical study of the effect of Nephrolepis biserrata pretreatment on exo-polygalacturonase production by Aspergilllus niger in solid state fermentation. EurAsian Journal of BioSciences, 13 (1). pp. 199-206. ISSN 1307-9867 http://www.ejobios.org/download/biochemical-study-of-the-effect-of-nephrolepis-biserrata-pretreatment-on-exo-polygalacturonase-6193.pdf.
institution Universiti Teknologi Malaysia
building UTM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Malaysia
content_source UTM Institutional Repository
url_provider http://eprints.utm.my/
topic TP Chemical technology
spellingShingle TP Chemical technology
Pagarra, H.
Rahman, R. A.
Rachmawaty, Rachmawaty
Biochemical study of the effect of Nephrolepis biserrata pretreatment on exo-polygalacturonase production by Aspergilllus niger in solid state fermentation
description The substrate plays an important role in solid state fermentation (SSF) in the production of exo-polygalacturonase (one type of the pectinase enzymes). The aim of this study was to investigate a pretreatment method on Nephrolepis biserrata leaves as a substrate for the production of exo-polygalacturonase. The pretreatment steps improved the decomposition of the lignocellulosic material in the structure of N. biserrata leaves as a substrate and allowed Aspergillus niger better access of the substrate to the enzymatic reaction. This optimised both exo-polygalacturonase activity and the growth of A. niger in SSF. Pretreatment was performed under alkali, acidic, autohydrolytic and control conditions. The highest exo-polygalacturonase activity was 39.16 U/g and biomass of A. niger was 0.39 mg/gdsf at 120 h was found for the autohydrolysis pretreatment, as compared to alkali pretreatment, acid pretreatment and control. Scanning electron microscope images showed a marked change in the physical appearance of treated N. biserrata leaves as compared to untreated N. biserrata leaves, especially following autohydrolysis pretreatment.
format Article
author Pagarra, H.
Rahman, R. A.
Rachmawaty, Rachmawaty
author_facet Pagarra, H.
Rahman, R. A.
Rachmawaty, Rachmawaty
author_sort Pagarra, H.
title Biochemical study of the effect of Nephrolepis biserrata pretreatment on exo-polygalacturonase production by Aspergilllus niger in solid state fermentation
title_short Biochemical study of the effect of Nephrolepis biserrata pretreatment on exo-polygalacturonase production by Aspergilllus niger in solid state fermentation
title_full Biochemical study of the effect of Nephrolepis biserrata pretreatment on exo-polygalacturonase production by Aspergilllus niger in solid state fermentation
title_fullStr Biochemical study of the effect of Nephrolepis biserrata pretreatment on exo-polygalacturonase production by Aspergilllus niger in solid state fermentation
title_full_unstemmed Biochemical study of the effect of Nephrolepis biserrata pretreatment on exo-polygalacturonase production by Aspergilllus niger in solid state fermentation
title_sort biochemical study of the effect of nephrolepis biserrata pretreatment on exo-polygalacturonase production by aspergilllus niger in solid state fermentation
publisher Foundation for Enviromental Protection and Research
publishDate 2019
url http://eprints.utm.my/id/eprint/91467/
http://www.ejobios.org/download/biochemical-study-of-the-effect-of-nephrolepis-biserrata-pretreatment-on-exo-polygalacturonase-6193.pdf.
_version_ 1705056717818036224
score 13.211869

Similar Items