Staff View: Bioconversion of pineapple pomace for xylooligosaccharide synthesis using surface display of xylanase on Escherichia coli

Bioconversion of pineapple pomace for xylooligosaccharide synthesis using surface display of xylanase on Escherichia coli

Cell surface display of xylanase on Escherichia coli was used for the hydrolysis of hemicellulose from pineapple pomace. The feasibility of bioconversion of lignocellulosic biomass into xylooligosaccharides (XOS) was investigated. In this study, pineapple pomace was pretreated, and the hemicellulose...

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Main Authors:	Wee, Mei Yuin Joanne, Wan Azelee, Nur Izyan, Pachelles, Samson, Abd. Murad, Abdul Munir, Abu Bakar, Farah Diba, Md. Illias, Rosli
Format:	Article
Published:	Springer Science and Business Media Deutschland GmbH 2020
Subjects:	TP Chemical technology
Online Access:	http://eprints.utm.my/id/eprint/90834/ http://dx.doi.org/10.1007/s13399-020-01041-0
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id	my.utm.90834
record_format	eprints
spelling	my.utm.908342021-05-31T13:21:57Z http://eprints.utm.my/id/eprint/90834/ Bioconversion of pineapple pomace for xylooligosaccharide synthesis using surface display of xylanase on Escherichia coli Wee, Mei Yuin Joanne Wan Azelee, Nur Izyan Pachelles, Samson Abd. Murad, Abdul Munir Abu Bakar, Farah Diba Md. Illias, Rosli TP Chemical technology Cell surface display of xylanase on Escherichia coli was used for the hydrolysis of hemicellulose from pineapple pomace. The feasibility of bioconversion of lignocellulosic biomass into xylooligosaccharides (XOS) was investigated. In this study, pineapple pomace was pretreated, and the hemicellulose fraction was obtained for reaction with the whole-cell biocatalyst. FESEM and FTIR analyses were used to observe morphological and compositional changes of pineapple pomace respectively after pretreatment. Factors affecting hydrolysis reaction were investigated and optimized using the Box-Behnken Design. The highest amount of reducing sugar was produced at pH 7.5, cell loading of 100 g/L wet cell weight, and temperature of 30 °C. The amount of reducing sugar produced was 2.129 mg/ml. HPLC analysis indicated that the XOS produced were xylobiose and xylotriose with a total yield of 5.4 mg/g of pineapple hemicellulose. FESEM analysis on the surface structure of pineapple pomace after the hydrolysis reaction showed clear signs of degradation by xylanase. Based on the results presented, it can be deduced that the application of cell surface display on E. coli for degradation of lignocellulosic biomass is possible and should be explored as it offers great potential for the production of XOS in industry.L Springer Science and Business Media Deutschland GmbH 2020 Article PeerReviewed Wee, Mei Yuin Joanne and Wan Azelee, Nur Izyan and Pachelles, Samson and Abd. Murad, Abdul Munir and Abu Bakar, Farah Diba and Md. Illias, Rosli (2020) Bioconversion of pineapple pomace for xylooligosaccharide synthesis using surface display of xylanase on Escherichia coli. Biomass Conversion and Biorefinery . ISSN 2190-6815 http://dx.doi.org/10.1007/s13399-020-01041-0
institution	Universiti Teknologi Malaysia
building	UTM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Teknologi Malaysia
content_source	UTM Institutional Repository
url_provider	http://eprints.utm.my/
topic	TP Chemical technology
spellingShingle	TP Chemical technology Wee, Mei Yuin Joanne Wan Azelee, Nur Izyan Pachelles, Samson Abd. Murad, Abdul Munir Abu Bakar, Farah Diba Md. Illias, Rosli Bioconversion of pineapple pomace for xylooligosaccharide synthesis using surface display of xylanase on Escherichia coli
description	Cell surface display of xylanase on Escherichia coli was used for the hydrolysis of hemicellulose from pineapple pomace. The feasibility of bioconversion of lignocellulosic biomass into xylooligosaccharides (XOS) was investigated. In this study, pineapple pomace was pretreated, and the hemicellulose fraction was obtained for reaction with the whole-cell biocatalyst. FESEM and FTIR analyses were used to observe morphological and compositional changes of pineapple pomace respectively after pretreatment. Factors affecting hydrolysis reaction were investigated and optimized using the Box-Behnken Design. The highest amount of reducing sugar was produced at pH 7.5, cell loading of 100 g/L wet cell weight, and temperature of 30 °C. The amount of reducing sugar produced was 2.129 mg/ml. HPLC analysis indicated that the XOS produced were xylobiose and xylotriose with a total yield of 5.4 mg/g of pineapple hemicellulose. FESEM analysis on the surface structure of pineapple pomace after the hydrolysis reaction showed clear signs of degradation by xylanase. Based on the results presented, it can be deduced that the application of cell surface display on E. coli for degradation of lignocellulosic biomass is possible and should be explored as it offers great potential for the production of XOS in industry.L
format	Article
author	Wee, Mei Yuin Joanne Wan Azelee, Nur Izyan Pachelles, Samson Abd. Murad, Abdul Munir Abu Bakar, Farah Diba Md. Illias, Rosli
author_facet	Wee, Mei Yuin Joanne Wan Azelee, Nur Izyan Pachelles, Samson Abd. Murad, Abdul Munir Abu Bakar, Farah Diba Md. Illias, Rosli
author_sort	Wee, Mei Yuin Joanne
title	Bioconversion of pineapple pomace for xylooligosaccharide synthesis using surface display of xylanase on Escherichia coli
title_short	Bioconversion of pineapple pomace for xylooligosaccharide synthesis using surface display of xylanase on Escherichia coli
title_full	Bioconversion of pineapple pomace for xylooligosaccharide synthesis using surface display of xylanase on Escherichia coli
title_fullStr	Bioconversion of pineapple pomace for xylooligosaccharide synthesis using surface display of xylanase on Escherichia coli
title_full_unstemmed	Bioconversion of pineapple pomace for xylooligosaccharide synthesis using surface display of xylanase on Escherichia coli
title_sort	bioconversion of pineapple pomace for xylooligosaccharide synthesis using surface display of xylanase on escherichia coli
publisher	Springer Science and Business Media Deutschland GmbH
publishDate	2020
url	http://eprints.utm.my/id/eprint/90834/ http://dx.doi.org/10.1007/s13399-020-01041-0
_version_	1702169608295809024
score	13.211869

Bioconversion of pineapple pomace for xylooligosaccharide synthesis using surface display of xylanase on Escherichia coli

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