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Rhizobacteria isolated from saline soil induce systemic tolerance in wheat (triticum aestivum l.) against salinity stress

Halotolerant plant growthpromoting rhizobacteria (PGPR) have the inherent potential to cope up with salinity. Thus, they can be used as an effective strategy in enhancing the productivity of saline agrosystems. In this study, a total of 50 isolates were screened from the rhizospheric soil of plants...

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Main Authors: Ilyas, Noshin, Mazhar, Roomina, Yasmin, Humaira, Khan, Wajiha, Iqbal, Sumera, El Enshasy, Hesham, Dailin, Daniel Joe
Format: Article
Language:English
Published: MDPI AG 2020
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TP Chemical technology
Online Access:http://eprints.utm.my/id/eprint/90514/1/HeshamElEnshasy2020_RhizobacteriaIsolatedfromSalineSoilInduce.pdf
http://eprints.utm.my/id/eprint/90514/
http://dx.doi.org/10.3390/agronomy10070989
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spelling my.utm.905142021-04-30T14:55:08Z http://eprints.utm.my/id/eprint/90514/ Rhizobacteria isolated from saline soil induce systemic tolerance in wheat (triticum aestivum l.) against salinity stress Ilyas, Noshin Mazhar, Roomina Yasmin, Humaira Khan, Wajiha Iqbal, Sumera El Enshasy, Hesham Dailin, Daniel Joe TP Chemical technology Halotolerant plant growthpromoting rhizobacteria (PGPR) have the inherent potential to cope up with salinity. Thus, they can be used as an effective strategy in enhancing the productivity of saline agrosystems. In this study, a total of 50 isolates were screened from the rhizospheric soil of plants growing in the salt range of Pakistan. Out of these, four isolates were selected based on their salinity tolerance and plant growth promotion characters. These isolates (SR1. SR2, SR3, and SR4) were identified as Bacillus sp. (KF719179), Azospirillum brasilense (KJ194586), Azospirillum lipoferum (KJ434039), and Pseudomonas stutzeri (KJ685889) by 16S rDNA gene sequence analysis. In vitro, these strains, in alone and in a consortium, showed better production of compatible solute and phytohormones, including indole acetic acid (IAA), gibberellic acid (GA), cytokinin (CK), and abscisic acid (ABA), in culture conditions under salt stress. When tested for inoculation, the consortium of all four strains showed the best results in terms of improved plant biomass and relative water content. Consortiuminoculated wheat plants showed tolerance by reduced electrolyte leakage and increased production of chlorophyll a, b, and total chlorophyll, and osmolytes, including soluble sugar, proline, amino acids, and antioxidant enzymes (superoxide dismutase, catalase, peroxidase), upon exposure to salinity stress (150 mM NaCl). In conclusion, plant growthpromoting bacteria, isolated from saltaffected regions, have strong potential to mitigate the deleterious effects of salt stress in wheat crop, when inoculated. Therefore, this consortium can be used as potent inoculants for wheat crop under prevailing stress conditions. MDPI AG 2020-07 Article PeerReviewed application/pdf en http://eprints.utm.my/id/eprint/90514/1/HeshamElEnshasy2020_RhizobacteriaIsolatedfromSalineSoilInduce.pdf Ilyas, Noshin and Mazhar, Roomina and Yasmin, Humaira and Khan, Wajiha and Iqbal, Sumera and El Enshasy, Hesham and Dailin, Daniel Joe (2020) Rhizobacteria isolated from saline soil induce systemic tolerance in wheat (triticum aestivum l.) against salinity stress. Agronomy, 10 (7). pp. 1-19. ISSN 2073-4395 http://dx.doi.org/10.3390/agronomy10070989 DOI:10.3390/agronomy10070989
institution Universiti Teknologi Malaysia
building UTM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Malaysia
content_source UTM Institutional Repository
url_provider http://eprints.utm.my/
language English
topic TP Chemical technology
spellingShingle TP Chemical technology
Ilyas, Noshin
Mazhar, Roomina
Yasmin, Humaira
Khan, Wajiha
Iqbal, Sumera
El Enshasy, Hesham
Dailin, Daniel Joe
Rhizobacteria isolated from saline soil induce systemic tolerance in wheat (triticum aestivum l.) against salinity stress
description Halotolerant plant growthpromoting rhizobacteria (PGPR) have the inherent potential to cope up with salinity. Thus, they can be used as an effective strategy in enhancing the productivity of saline agrosystems. In this study, a total of 50 isolates were screened from the rhizospheric soil of plants growing in the salt range of Pakistan. Out of these, four isolates were selected based on their salinity tolerance and plant growth promotion characters. These isolates (SR1. SR2, SR3, and SR4) were identified as Bacillus sp. (KF719179), Azospirillum brasilense (KJ194586), Azospirillum lipoferum (KJ434039), and Pseudomonas stutzeri (KJ685889) by 16S rDNA gene sequence analysis. In vitro, these strains, in alone and in a consortium, showed better production of compatible solute and phytohormones, including indole acetic acid (IAA), gibberellic acid (GA), cytokinin (CK), and abscisic acid (ABA), in culture conditions under salt stress. When tested for inoculation, the consortium of all four strains showed the best results in terms of improved plant biomass and relative water content. Consortiuminoculated wheat plants showed tolerance by reduced electrolyte leakage and increased production of chlorophyll a, b, and total chlorophyll, and osmolytes, including soluble sugar, proline, amino acids, and antioxidant enzymes (superoxide dismutase, catalase, peroxidase), upon exposure to salinity stress (150 mM NaCl). In conclusion, plant growthpromoting bacteria, isolated from saltaffected regions, have strong potential to mitigate the deleterious effects of salt stress in wheat crop, when inoculated. Therefore, this consortium can be used as potent inoculants for wheat crop under prevailing stress conditions.
format Article
author Ilyas, Noshin
Mazhar, Roomina
Yasmin, Humaira
Khan, Wajiha
Iqbal, Sumera
El Enshasy, Hesham
Dailin, Daniel Joe
author_facet Ilyas, Noshin
Mazhar, Roomina
Yasmin, Humaira
Khan, Wajiha
Iqbal, Sumera
El Enshasy, Hesham
Dailin, Daniel Joe
author_sort Ilyas, Noshin
title Rhizobacteria isolated from saline soil induce systemic tolerance in wheat (triticum aestivum l.) against salinity stress
title_short Rhizobacteria isolated from saline soil induce systemic tolerance in wheat (triticum aestivum l.) against salinity stress
title_full Rhizobacteria isolated from saline soil induce systemic tolerance in wheat (triticum aestivum l.) against salinity stress
title_fullStr Rhizobacteria isolated from saline soil induce systemic tolerance in wheat (triticum aestivum l.) against salinity stress
title_full_unstemmed Rhizobacteria isolated from saline soil induce systemic tolerance in wheat (triticum aestivum l.) against salinity stress
title_sort rhizobacteria isolated from saline soil induce systemic tolerance in wheat (triticum aestivum l.) against salinity stress
publisher MDPI AG
publishDate 2020
url http://eprints.utm.my/id/eprint/90514/1/HeshamElEnshasy2020_RhizobacteriaIsolatedfromSalineSoilInduce.pdf
http://eprints.utm.my/id/eprint/90514/
http://dx.doi.org/10.3390/agronomy10070989
_version_ 1698696947115753472
score 13.211869

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