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Statistical modelling of ultrasonic-aided extraction of elaeis guineensis leaves for better-quality yield and total phenolic content

The present study highlighted the statistical modelling of an ultrasonic-aided extraction (UAE) of Elaeis guineensis leaves extract for maximal extraction yield (EY) and total phenolic content (TPC). A Box-Behnken design investigated the effects of ethanol concentration (X1: 0−100%), extraction time...

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Main Authors: Romes, Nissha Bharrathi, Abdul Hamid, Mariani, Hashim, Siti Ernieyanti, Abdul Wahab, Roswanira
Format: Article
Language:English
Published: Gadjah Mada University 2019
Subjects:
QD Chemistry
Online Access:http://eprints.utm.my/id/eprint/89029/1/RoswaniraAbWahab2019_StatisticalModellingofUltrasonic-AidedExtraction.pdf
http://eprints.utm.my/id/eprint/89029/
http://dx.doi.org/10.22146/ijc.41603
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spelling my.utm.890292021-01-26T08:41:34Z http://eprints.utm.my/id/eprint/89029/ Statistical modelling of ultrasonic-aided extraction of elaeis guineensis leaves for better-quality yield and total phenolic content Romes, Nissha Bharrathi Abdul Hamid, Mariani Hashim, Siti Ernieyanti Abdul Wahab, Roswanira QD Chemistry The present study highlighted the statistical modelling of an ultrasonic-aided extraction (UAE) of Elaeis guineensis leaves extract for maximal extraction yield (EY) and total phenolic content (TPC). A Box-Behnken design investigated the effects of ethanol concentration (X1: 0−100%), extraction time (X2: 5−55 min), the solvent-to-solid ratio (X3: 15:1−35:1 mL/g) and sonification amplitude (X4: 20−100 %). Under optimized conditions, the highest EY of 14.38% was attained using 50% (v/v) ethanol:water ratio, 55 min, 35 mL/g solvent-to-solid ratio, 60% sonication amplitude, whereas maximum TPC was 209.70 mg gallic acid equivalent (GAE)/g [50% (v/v) ethanol:water ratio, 30 min, 25 mL/g solvent-to-solid ratio, 60% sonication amplitude]. Second-order polynomial models of EY and TPC showed the R2 value corresponding to 0.9303 and 0.9500, respectively, indicating their significance (p < 0.05) to predict the responses. HPLC chromatograms revealed gallic acid and catechin were present in the UAE extracts. UAE technique afforded better EY (14.38%) and TPC (209.70 mg GAE/g) than maceration (3.73%, 85.23 mg GAE/g) and Soxhlet (6.86%, 102.13 mg GAE/g) extractions, as based on scanning electron micrographs of untreated, UAE, macerated and Soxhlet treated samples. Cell walls of ultrasonic-treated E. guineensis leaves were visibly disrupted to facilitate the higher release of bioactive plant materials, thus justifying the higher EY and TPC. The application of ultrasound appeared to remarkably increase the extraction efficiency of E. guineensis leaves to extract as compared to the conventional methods. Gadjah Mada University 2019 Article PeerReviewed application/pdf en http://eprints.utm.my/id/eprint/89029/1/RoswaniraAbWahab2019_StatisticalModellingofUltrasonic-AidedExtraction.pdf Romes, Nissha Bharrathi and Abdul Hamid, Mariani and Hashim, Siti Ernieyanti and Abdul Wahab, Roswanira (2019) Statistical modelling of ultrasonic-aided extraction of elaeis guineensis leaves for better-quality yield and total phenolic content. Indonesian Journal of Chemistry, 19 (3). pp. 811-826. ISSN 1411-9420 http://dx.doi.org/10.22146/ijc.41603
institution Universiti Teknologi Malaysia
building UTM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Malaysia
content_source UTM Institutional Repository
url_provider http://eprints.utm.my/
language English
topic QD Chemistry
spellingShingle QD Chemistry
Romes, Nissha Bharrathi
Abdul Hamid, Mariani
Hashim, Siti Ernieyanti
Abdul Wahab, Roswanira
Statistical modelling of ultrasonic-aided extraction of elaeis guineensis leaves for better-quality yield and total phenolic content
description The present study highlighted the statistical modelling of an ultrasonic-aided extraction (UAE) of Elaeis guineensis leaves extract for maximal extraction yield (EY) and total phenolic content (TPC). A Box-Behnken design investigated the effects of ethanol concentration (X1: 0−100%), extraction time (X2: 5−55 min), the solvent-to-solid ratio (X3: 15:1−35:1 mL/g) and sonification amplitude (X4: 20−100 %). Under optimized conditions, the highest EY of 14.38% was attained using 50% (v/v) ethanol:water ratio, 55 min, 35 mL/g solvent-to-solid ratio, 60% sonication amplitude, whereas maximum TPC was 209.70 mg gallic acid equivalent (GAE)/g [50% (v/v) ethanol:water ratio, 30 min, 25 mL/g solvent-to-solid ratio, 60% sonication amplitude]. Second-order polynomial models of EY and TPC showed the R2 value corresponding to 0.9303 and 0.9500, respectively, indicating their significance (p < 0.05) to predict the responses. HPLC chromatograms revealed gallic acid and catechin were present in the UAE extracts. UAE technique afforded better EY (14.38%) and TPC (209.70 mg GAE/g) than maceration (3.73%, 85.23 mg GAE/g) and Soxhlet (6.86%, 102.13 mg GAE/g) extractions, as based on scanning electron micrographs of untreated, UAE, macerated and Soxhlet treated samples. Cell walls of ultrasonic-treated E. guineensis leaves were visibly disrupted to facilitate the higher release of bioactive plant materials, thus justifying the higher EY and TPC. The application of ultrasound appeared to remarkably increase the extraction efficiency of E. guineensis leaves to extract as compared to the conventional methods.
format Article
author Romes, Nissha Bharrathi
Abdul Hamid, Mariani
Hashim, Siti Ernieyanti
Abdul Wahab, Roswanira
author_facet Romes, Nissha Bharrathi
Abdul Hamid, Mariani
Hashim, Siti Ernieyanti
Abdul Wahab, Roswanira
author_sort Romes, Nissha Bharrathi
title Statistical modelling of ultrasonic-aided extraction of elaeis guineensis leaves for better-quality yield and total phenolic content
title_short Statistical modelling of ultrasonic-aided extraction of elaeis guineensis leaves for better-quality yield and total phenolic content
title_full Statistical modelling of ultrasonic-aided extraction of elaeis guineensis leaves for better-quality yield and total phenolic content
title_fullStr Statistical modelling of ultrasonic-aided extraction of elaeis guineensis leaves for better-quality yield and total phenolic content
title_full_unstemmed Statistical modelling of ultrasonic-aided extraction of elaeis guineensis leaves for better-quality yield and total phenolic content
title_sort statistical modelling of ultrasonic-aided extraction of elaeis guineensis leaves for better-quality yield and total phenolic content
publisher Gadjah Mada University
publishDate 2019
url http://eprints.utm.my/id/eprint/89029/1/RoswaniraAbWahab2019_StatisticalModellingofUltrasonic-AidedExtraction.pdf
http://eprints.utm.my/id/eprint/89029/
http://dx.doi.org/10.22146/ijc.41603
_version_ 1690370959500902400
score 13.251813

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