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A TonB-dependent receptor constitutes the outer membrane transport system for a lignin-derived aromatic compound

TonB-dependent receptors (TBDRs) mediate substrate-specific transport across the outer membrane, utilizing energy derived from the proton motive force transmitted from the TonB−ExbB−ExbD complex located in the inner membrane (TonB system). Although a number of TonB systems involved in the uptake of...

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Main Authors: Fujita, Masaya, Mori, Kosuke, Hara, Hirofumi, Hishiyama, Shojiro, Kamimura, Naofumi, Masai, Eiji
Format: Article
Language:English
Published: Nature Research 2019
Subjects:
T Technology (General)
Online Access:http://eprints.utm.my/id/eprint/88662/1/HirofumiHara2019_ATonB-DependentReceptorConstitutestheOuterMembrane.pdf
http://eprints.utm.my/id/eprint/88662/
http://dx.doi.org/10.1038/s42003-019-0676-z
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spelling my.utm.886622020-12-15T10:53:42Z http://eprints.utm.my/id/eprint/88662/ A TonB-dependent receptor constitutes the outer membrane transport system for a lignin-derived aromatic compound Fujita, Masaya Mori, Kosuke Hara, Hirofumi Hishiyama, Shojiro Kamimura, Naofumi Masai, Eiji T Technology (General) TonB-dependent receptors (TBDRs) mediate substrate-specific transport across the outer membrane, utilizing energy derived from the proton motive force transmitted from the TonB−ExbB−ExbD complex located in the inner membrane (TonB system). Although a number of TonB systems involved in the uptake of siderophores, vitamin B12 and saccharides have been identified, their involvement in the uptake and catabolism of aromatic compounds was previously unknown. Here, we show that the outer membrane transport of a biphenyl compound derived from lignin is mediated by the TonB system in a Gram-negative bacterium capable of degrading lignin-derived aromatic compounds, Sphingobium sp. strain SYK-6. Furthermore, we found that overexpression of the corresponding TBDR gene enhanced the uptake of this biphenyl compound, contributing to the improved rate of platform chemical production. Our results will provide an important basis for establishing engineered strains optimized for use in lignin valorisation. Nature Research 2019-12 Article PeerReviewed application/pdf en http://eprints.utm.my/id/eprint/88662/1/HirofumiHara2019_ATonB-DependentReceptorConstitutestheOuterMembrane.pdf Fujita, Masaya and Mori, Kosuke and Hara, Hirofumi and Hishiyama, Shojiro and Kamimura, Naofumi and Masai, Eiji (2019) A TonB-dependent receptor constitutes the outer membrane transport system for a lignin-derived aromatic compound. Communications Biology, 2 (1). p. 432. ISSN 2399-3642 http://dx.doi.org/10.1038/s42003-019-0676-z
institution Universiti Teknologi Malaysia
building UTM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Malaysia
content_source UTM Institutional Repository
url_provider http://eprints.utm.my/
language English
topic T Technology (General)
spellingShingle T Technology (General)
Fujita, Masaya
Mori, Kosuke
Hara, Hirofumi
Hishiyama, Shojiro
Kamimura, Naofumi
Masai, Eiji
A TonB-dependent receptor constitutes the outer membrane transport system for a lignin-derived aromatic compound
description TonB-dependent receptors (TBDRs) mediate substrate-specific transport across the outer membrane, utilizing energy derived from the proton motive force transmitted from the TonB−ExbB−ExbD complex located in the inner membrane (TonB system). Although a number of TonB systems involved in the uptake of siderophores, vitamin B12 and saccharides have been identified, their involvement in the uptake and catabolism of aromatic compounds was previously unknown. Here, we show that the outer membrane transport of a biphenyl compound derived from lignin is mediated by the TonB system in a Gram-negative bacterium capable of degrading lignin-derived aromatic compounds, Sphingobium sp. strain SYK-6. Furthermore, we found that overexpression of the corresponding TBDR gene enhanced the uptake of this biphenyl compound, contributing to the improved rate of platform chemical production. Our results will provide an important basis for establishing engineered strains optimized for use in lignin valorisation.
format Article
author Fujita, Masaya
Mori, Kosuke
Hara, Hirofumi
Hishiyama, Shojiro
Kamimura, Naofumi
Masai, Eiji
author_facet Fujita, Masaya
Mori, Kosuke
Hara, Hirofumi
Hishiyama, Shojiro
Kamimura, Naofumi
Masai, Eiji
author_sort Fujita, Masaya
title A TonB-dependent receptor constitutes the outer membrane transport system for a lignin-derived aromatic compound
title_short A TonB-dependent receptor constitutes the outer membrane transport system for a lignin-derived aromatic compound
title_full A TonB-dependent receptor constitutes the outer membrane transport system for a lignin-derived aromatic compound
title_fullStr A TonB-dependent receptor constitutes the outer membrane transport system for a lignin-derived aromatic compound
title_full_unstemmed A TonB-dependent receptor constitutes the outer membrane transport system for a lignin-derived aromatic compound
title_sort tonb-dependent receptor constitutes the outer membrane transport system for a lignin-derived aromatic compound
publisher Nature Research
publishDate 2019
url http://eprints.utm.my/id/eprint/88662/1/HirofumiHara2019_ATonB-DependentReceptorConstitutestheOuterMembrane.pdf
http://eprints.utm.my/id/eprint/88662/
http://dx.doi.org/10.1038/s42003-019-0676-z
_version_ 1687393603263922176
score 13.211869

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