Staff View: Optimization of ultrasound-assisted extraction conditions followed by solid phase extraction fractionation from Orthosiphon stamineus benth (Lamiace) leaves for antiproliferative effect on prostate cancer cells

Optimization of ultrasound-assisted extraction conditions followed by solid phase extraction fractionation from Orthosiphon stamineus benth (Lamiace) leaves for antiproliferative effect on prostate cancer cells

Primarily, optimization of ultrasonic-assisted extraction (UAE) conditions of Orthospihon stamineus was evaluated and verified using a central composite design (CCD) based on three factors including extraction time (minutes), ultrasound amplitude (A), and solvent concentration (%). The response surf...

Full description

Saved in:

Bibliographic Details
Main Authors:	Suhaimi, Siti Hasyimah, Hasham, Rosnani, Idris, Mohamad Khairul Hafiz, Ismail, Hassan Fahmi, Mohd. Ariffin, Nor Hazwani, Abdul Majid, Fadzilah Adibah
Format:	Article
Language:	English
Published:	MDPI AG 2019
Subjects:	TP Chemical technology
Online Access:	http://eprints.utm.my/id/eprint/88611/1/RosnaniHasham2019_OptimizationofUltrasound-AssistedExtractionConditions.pdf http://eprints.utm.my/id/eprint/88611/ http://dx.doi.org/10.3390/molecules24224183
Tags:	Add Tag No Tags, Be the first to tag this record!

id	my.utm.88611
record_format	eprints
spelling	my.utm.886112020-12-15T10:35:25Z http://eprints.utm.my/id/eprint/88611/ Optimization of ultrasound-assisted extraction conditions followed by solid phase extraction fractionation from Orthosiphon stamineus benth (Lamiace) leaves for antiproliferative effect on prostate cancer cells Suhaimi, Siti Hasyimah Hasham, Rosnani Idris, Mohamad Khairul Hafiz Ismail, Hassan Fahmi Mohd. Ariffin, Nor Hazwani Abdul Majid, Fadzilah Adibah TP Chemical technology Primarily, optimization of ultrasonic-assisted extraction (UAE) conditions of Orthospihon stamineus was evaluated and verified using a central composite design (CCD) based on three factors including extraction time (minutes), ultrasound amplitude (A), and solvent concentration (%). The response surfacemethodology (RSM)was performed to develop an extractionmethodwithmaximum yield and high rosmarinic acid content. The optimal UAE conditions were as follows: extraction time 21min, ultrasound amplitudes 62A, and solvent composition 70%ethanol inwater. The crude extractwas further fractionated using solid-phase extraction (SPE),where six sequential fractions that varied in polarity (0-100% Acetonitrile in water) were obtained. Next, the six fractions were evaluated for their antioxidant and anti-cancer properties. This study found that Fraction 2 (F2) contained the highest rosmarinic acid content and showed the strongest antioxidant activity. Additionally, F2 showed an anti-proliferative effect against prostate cancer (DU145) with no harmful effect on normal cells. MDPI AG 2019-11 Article PeerReviewed application/pdf en http://eprints.utm.my/id/eprint/88611/1/RosnaniHasham2019_OptimizationofUltrasound-AssistedExtractionConditions.pdf Suhaimi, Siti Hasyimah and Hasham, Rosnani and Idris, Mohamad Khairul Hafiz and Ismail, Hassan Fahmi and Mohd. Ariffin, Nor Hazwani and Abdul Majid, Fadzilah Adibah (2019) Optimization of ultrasound-assisted extraction conditions followed by solid phase extraction fractionation from Orthosiphon stamineus benth (Lamiace) leaves for antiproliferative effect on prostate cancer cells. Molecules, 24 (22). p. 4183. ISSN 1420-3049 http://dx.doi.org/10.3390/molecules24224183
institution	Universiti Teknologi Malaysia
building	UTM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Teknologi Malaysia
content_source	UTM Institutional Repository
url_provider	http://eprints.utm.my/
language	English
topic	TP Chemical technology
spellingShingle	TP Chemical technology Suhaimi, Siti Hasyimah Hasham, Rosnani Idris, Mohamad Khairul Hafiz Ismail, Hassan Fahmi Mohd. Ariffin, Nor Hazwani Abdul Majid, Fadzilah Adibah Optimization of ultrasound-assisted extraction conditions followed by solid phase extraction fractionation from Orthosiphon stamineus benth (Lamiace) leaves for antiproliferative effect on prostate cancer cells
description	Primarily, optimization of ultrasonic-assisted extraction (UAE) conditions of Orthospihon stamineus was evaluated and verified using a central composite design (CCD) based on three factors including extraction time (minutes), ultrasound amplitude (A), and solvent concentration (%). The response surfacemethodology (RSM)was performed to develop an extractionmethodwithmaximum yield and high rosmarinic acid content. The optimal UAE conditions were as follows: extraction time 21min, ultrasound amplitudes 62A, and solvent composition 70%ethanol inwater. The crude extractwas further fractionated using solid-phase extraction (SPE),where six sequential fractions that varied in polarity (0-100% Acetonitrile in water) were obtained. Next, the six fractions were evaluated for their antioxidant and anti-cancer properties. This study found that Fraction 2 (F2) contained the highest rosmarinic acid content and showed the strongest antioxidant activity. Additionally, F2 showed an anti-proliferative effect against prostate cancer (DU145) with no harmful effect on normal cells.
format	Article
author	Suhaimi, Siti Hasyimah Hasham, Rosnani Idris, Mohamad Khairul Hafiz Ismail, Hassan Fahmi Mohd. Ariffin, Nor Hazwani Abdul Majid, Fadzilah Adibah
author_facet	Suhaimi, Siti Hasyimah Hasham, Rosnani Idris, Mohamad Khairul Hafiz Ismail, Hassan Fahmi Mohd. Ariffin, Nor Hazwani Abdul Majid, Fadzilah Adibah
author_sort	Suhaimi, Siti Hasyimah
title	Optimization of ultrasound-assisted extraction conditions followed by solid phase extraction fractionation from Orthosiphon stamineus benth (Lamiace) leaves for antiproliferative effect on prostate cancer cells
title_short	Optimization of ultrasound-assisted extraction conditions followed by solid phase extraction fractionation from Orthosiphon stamineus benth (Lamiace) leaves for antiproliferative effect on prostate cancer cells
title_full	Optimization of ultrasound-assisted extraction conditions followed by solid phase extraction fractionation from Orthosiphon stamineus benth (Lamiace) leaves for antiproliferative effect on prostate cancer cells
title_fullStr	Optimization of ultrasound-assisted extraction conditions followed by solid phase extraction fractionation from Orthosiphon stamineus benth (Lamiace) leaves for antiproliferative effect on prostate cancer cells
title_full_unstemmed	Optimization of ultrasound-assisted extraction conditions followed by solid phase extraction fractionation from Orthosiphon stamineus benth (Lamiace) leaves for antiproliferative effect on prostate cancer cells
title_sort	optimization of ultrasound-assisted extraction conditions followed by solid phase extraction fractionation from orthosiphon stamineus benth (lamiace) leaves for antiproliferative effect on prostate cancer cells
publisher	MDPI AG
publishDate	2019
url	http://eprints.utm.my/id/eprint/88611/1/RosnaniHasham2019_OptimizationofUltrasound-AssistedExtractionConditions.pdf http://eprints.utm.my/id/eprint/88611/ http://dx.doi.org/10.3390/molecules24224183
_version_	1687393595651260416
score	13.211869

Optimization of ultrasound-assisted extraction conditions followed by solid phase extraction fractionation from Orthosiphon stamineus benth (Lamiace) leaves for antiproliferative effect on prostate cancer cells

Similar Items