Staff View: Optimization of lipase immobilization on maghemite and its physico-chemical properties

Optimization of lipase immobilization on maghemite and its physico-chemical properties

Nanomaterial-based biocatalysts have emerged as current carriers suitable for enzyme immobilization. The nano-sized materials provide large surface area for enzyme attachment, thus increasing the probability for its effcient catalyst activity. By using magnetized nanomaterials, enhancement of the do...

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Main Authors:	Ariffin, Maryam F. K., Idris, Ani, Ngadiman, Nor H. A.
Format:	Article
Language:	English
Published:	Assoc. Brasiliera de Eng. Quimica / Braz. Soc. Chem. Eng. 2019
Subjects:	TP Chemical technology
Online Access:	http://eprints.utm.my/id/eprint/88411/1/AniIdris2019_OptimizationofLipaseImmobilizationonMaghemiteanditsPhysico-ChemicalProperties.pdf http://eprints.utm.my/id/eprint/88411/ http://dx.doi.org/10.1590/0104-6632.20190361s20180168
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spelling	my.utm.884112020-12-15T00:06:04Z http://eprints.utm.my/id/eprint/88411/ Optimization of lipase immobilization on maghemite and its physico-chemical properties Ariffin, Maryam F. K. Idris, Ani Ngadiman, Nor H. A. TP Chemical technology Nanomaterial-based biocatalysts have emerged as current carriers suitable for enzyme immobilization. The nano-sized materials provide large surface area for enzyme attachment, thus increasing the probability for its effcient catalyst activity. By using magnetized nanomaterials, enhancement of the downstream processing is evident as it eases the immobilized enzyme separation from the reaction mixture further. Lipase / maghemite composites were prepared by initial maghemite surface modifcation to cater to the needs for biocatalyst attachment. Surface modifcation using chitosan and subsequent cross-linking with glutaraldehyde provide a suitable environment for the enzyme to be immobilized. Optimization of the conditions for lipase immobilization was carried out using a response surface methodology (RSM) experimental design to obtain the precise optimized conditions for the process. Selected process variables involved were chosen and optimized conditions for lipase immobilization were 9 hour incubation time, 55°C incubation temperature and 12 % (v/v) glutaraldehyde content. The optimized immobilized lipase activity was 1.8 U. Characterizations of the on synthesized materials were also performed. The size distribution of maghemite nanomaterials was mainly within the range of 2-3 nm. Thermal properties of the synthesized maghemite was investigated using DSC and TGA analyses and we found that maghemite changes to hematite at 456.3°C. Magnetic properties of both untreated and lipase immobilized maghemite were studied using VSM and both were superparamagnetic nanomaterials with saturation magnetizations of 34.3 and 80.3, respectively. Assoc. Brasiliera de Eng. Quimica / Braz. Soc. Chem. Eng. 2019 Article PeerReviewed application/pdf en http://eprints.utm.my/id/eprint/88411/1/AniIdris2019_OptimizationofLipaseImmobilizationonMaghemiteanditsPhysico-ChemicalProperties.pdf Ariffin, Maryam F. K. and Idris, Ani and Ngadiman, Nor H. A. (2019) Optimization of lipase immobilization on maghemite and its physico-chemical properties. Brazilian Journal of Chemical Engineering, 36 (1). pp. 171-179. ISSN 0104-6632 http://dx.doi.org/10.1590/0104-6632.20190361s20180168
institution	Universiti Teknologi Malaysia
building	UTM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Teknologi Malaysia
content_source	UTM Institutional Repository
url_provider	http://eprints.utm.my/
language	English
topic	TP Chemical technology
spellingShingle	TP Chemical technology Ariffin, Maryam F. K. Idris, Ani Ngadiman, Nor H. A. Optimization of lipase immobilization on maghemite and its physico-chemical properties
description	Nanomaterial-based biocatalysts have emerged as current carriers suitable for enzyme immobilization. The nano-sized materials provide large surface area for enzyme attachment, thus increasing the probability for its effcient catalyst activity. By using magnetized nanomaterials, enhancement of the downstream processing is evident as it eases the immobilized enzyme separation from the reaction mixture further. Lipase / maghemite composites were prepared by initial maghemite surface modifcation to cater to the needs for biocatalyst attachment. Surface modifcation using chitosan and subsequent cross-linking with glutaraldehyde provide a suitable environment for the enzyme to be immobilized. Optimization of the conditions for lipase immobilization was carried out using a response surface methodology (RSM) experimental design to obtain the precise optimized conditions for the process. Selected process variables involved were chosen and optimized conditions for lipase immobilization were 9 hour incubation time, 55°C incubation temperature and 12 % (v/v) glutaraldehyde content. The optimized immobilized lipase activity was 1.8 U. Characterizations of the on synthesized materials were also performed. The size distribution of maghemite nanomaterials was mainly within the range of 2-3 nm. Thermal properties of the synthesized maghemite was investigated using DSC and TGA analyses and we found that maghemite changes to hematite at 456.3°C. Magnetic properties of both untreated and lipase immobilized maghemite were studied using VSM and both were superparamagnetic nanomaterials with saturation magnetizations of 34.3 and 80.3, respectively.
format	Article
author	Ariffin, Maryam F. K. Idris, Ani Ngadiman, Nor H. A.
author_facet	Ariffin, Maryam F. K. Idris, Ani Ngadiman, Nor H. A.
author_sort	Ariffin, Maryam F. K.
title	Optimization of lipase immobilization on maghemite and its physico-chemical properties
title_short	Optimization of lipase immobilization on maghemite and its physico-chemical properties
title_full	Optimization of lipase immobilization on maghemite and its physico-chemical properties
title_fullStr	Optimization of lipase immobilization on maghemite and its physico-chemical properties
title_full_unstemmed	Optimization of lipase immobilization on maghemite and its physico-chemical properties
title_sort	optimization of lipase immobilization on maghemite and its physico-chemical properties
publisher	Assoc. Brasiliera de Eng. Quimica / Braz. Soc. Chem. Eng.
publishDate	2019
url	http://eprints.utm.my/id/eprint/88411/1/AniIdris2019_OptimizationofLipaseImmobilizationonMaghemiteanditsPhysico-ChemicalProperties.pdf http://eprints.utm.my/id/eprint/88411/ http://dx.doi.org/10.1590/0104-6632.20190361s20180168
_version_	1687393568158646272
score	13.211869

Optimization of lipase immobilization on maghemite and its physico-chemical properties

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