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Hyperthermia effect on human normal breast (MCF-10A) and cancer (MDA-MB 231 and MCF-7) cells

Objective: In this study, the hyperthermia effect on the viability of human normal breast (MCF-10A) and cancer (MDA-MB 231 and MCF-7) cells was evaluated by MTT assay. Methods: Cells were exposed to heat at 38ºC, 39ºC, 40ºC, 41ºC, 42ºC, 43ºC, and 44ºC for five different durations of heat exposure (0...

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Main Authors: Elengoe, Asita, Mohammed Alitheen, Noor Jahan Banu, Hamdan, Salehhuddin
Format: Article
Language:English
Published: Innovare Academic Sciences 2019
Subjects:
QH301 Biology
Online Access:http://eprints.utm.my/id/eprint/88289/1/SalehhuddinHamdan2019_HyperthermiaEffectonHumanNormalBreast.pdf
http://eprints.utm.my/id/eprint/88289/
https://doi.org/10.22159/ajpcr.2019.v12i3.30956
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spelling my.utm.882892020-12-14T23:56:41Z http://eprints.utm.my/id/eprint/88289/ Hyperthermia effect on human normal breast (MCF-10A) and cancer (MDA-MB 231 and MCF-7) cells Elengoe, Asita Mohammed Alitheen, Noor Jahan Banu Hamdan, Salehhuddin QH301 Biology Objective: In this study, the hyperthermia effect on the viability of human normal breast (MCF-10A) and cancer (MDA-MB 231 and MCF-7) cells was evaluated by MTT assay. Methods: Cells were exposed to heat at 38ºC, 39ºC, 40ºC, 41ºC, 42ºC, 43ºC, and 44ºC for five different durations of heat exposure (0.5, 1, 2, 3, and 4 h). Breakpoint temperatures of MCF-10A, MDA-MB 231, and MCF-7 were determined using cumulative equivalent 43°C (CEM43) model. This model was first time used to calculate thermal isoeffect dose (TID) for MCF-10A, MDA-MB 231, and MCF-7. Results: MCF-10A started to die at 42°C for 3 h while MDA-MB 231 and MCF-7 need a temperature of 38°C for 0.5 h; thus, they were identified as the threshold temperatures in CEM43 model. Furthermore, the effect of “43°C incubator 2 h” had similar total thermal dose as “44°C incubator 0.5 h” for MDA-MB 231 and MCF-7. In addition, “43°C incubator 3 h” effect had also almost the same thermal dose as “44°C incubator 1 h” for MDA-MB 231 and MCF-7. Conclusion: A better understanding of the significant correlations between CEM43 and response parameters in clinical trials could be useful to treat breast cancer patients. Innovare Academic Sciences 2019 Article PeerReviewed application/pdf en http://eprints.utm.my/id/eprint/88289/1/SalehhuddinHamdan2019_HyperthermiaEffectonHumanNormalBreast.pdf Elengoe, Asita and Mohammed Alitheen, Noor Jahan Banu and Hamdan, Salehhuddin (2019) Hyperthermia effect on human normal breast (MCF-10A) and cancer (MDA-MB 231 and MCF-7) cells. Asian Journal of Pharmaceutical and Clinical Research, 12 (3). pp. 512-515. ISSN 0974-2441 https://doi.org/10.22159/ajpcr.2019.v12i3.30956 DOI:10.22159/ajpcr.2019.v12i3.30956
institution Universiti Teknologi Malaysia
building UTM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Malaysia
content_source UTM Institutional Repository
url_provider http://eprints.utm.my/
language English
topic QH301 Biology
spellingShingle QH301 Biology
Elengoe, Asita
Mohammed Alitheen, Noor Jahan Banu
Hamdan, Salehhuddin
Hyperthermia effect on human normal breast (MCF-10A) and cancer (MDA-MB 231 and MCF-7) cells
description Objective: In this study, the hyperthermia effect on the viability of human normal breast (MCF-10A) and cancer (MDA-MB 231 and MCF-7) cells was evaluated by MTT assay. Methods: Cells were exposed to heat at 38ºC, 39ºC, 40ºC, 41ºC, 42ºC, 43ºC, and 44ºC for five different durations of heat exposure (0.5, 1, 2, 3, and 4 h). Breakpoint temperatures of MCF-10A, MDA-MB 231, and MCF-7 were determined using cumulative equivalent 43°C (CEM43) model. This model was first time used to calculate thermal isoeffect dose (TID) for MCF-10A, MDA-MB 231, and MCF-7. Results: MCF-10A started to die at 42°C for 3 h while MDA-MB 231 and MCF-7 need a temperature of 38°C for 0.5 h; thus, they were identified as the threshold temperatures in CEM43 model. Furthermore, the effect of “43°C incubator 2 h” had similar total thermal dose as “44°C incubator 0.5 h” for MDA-MB 231 and MCF-7. In addition, “43°C incubator 3 h” effect had also almost the same thermal dose as “44°C incubator 1 h” for MDA-MB 231 and MCF-7. Conclusion: A better understanding of the significant correlations between CEM43 and response parameters in clinical trials could be useful to treat breast cancer patients.
format Article
author Elengoe, Asita
Mohammed Alitheen, Noor Jahan Banu
Hamdan, Salehhuddin
author_facet Elengoe, Asita
Mohammed Alitheen, Noor Jahan Banu
Hamdan, Salehhuddin
author_sort Elengoe, Asita
title Hyperthermia effect on human normal breast (MCF-10A) and cancer (MDA-MB 231 and MCF-7) cells
title_short Hyperthermia effect on human normal breast (MCF-10A) and cancer (MDA-MB 231 and MCF-7) cells
title_full Hyperthermia effect on human normal breast (MCF-10A) and cancer (MDA-MB 231 and MCF-7) cells
title_fullStr Hyperthermia effect on human normal breast (MCF-10A) and cancer (MDA-MB 231 and MCF-7) cells
title_full_unstemmed Hyperthermia effect on human normal breast (MCF-10A) and cancer (MDA-MB 231 and MCF-7) cells
title_sort hyperthermia effect on human normal breast (mcf-10a) and cancer (mda-mb 231 and mcf-7) cells
publisher Innovare Academic Sciences
publishDate 2019
url http://eprints.utm.my/id/eprint/88289/1/SalehhuddinHamdan2019_HyperthermiaEffectonHumanNormalBreast.pdf
http://eprints.utm.my/id/eprint/88289/
https://doi.org/10.22159/ajpcr.2019.v12i3.30956
_version_ 1687393551497822208
score 13.211869

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