Cover Image

Effect of operative variables and kinetic study of butyl butyrate synthesis by candida rugosa lipase activated by chitosan-reinforced nanocellulose derived from raw oil palm leaves

Currently, the chemically-assisted esterification to manufacture butyl butyrate employs corrosive homogeneous acid catalyst and liberates enormous quantities of hazardous by-products which complicate downstream treatment processes. This study aimed to identify the optimized esterification conditions...

Full description

Saved in:
Bibliographic Details
Main Authors: Elias, Nursyafiqah, Abdul Wahab, Roswanira, Chandren, Sheela, Abdul Razak, Fazira Ilyana, Jamalis, Joazaizulfazli
Format: Article
Published: Elsevier Inc. 2019
Subjects:
QD Chemistry
Online Access:http://eprints.utm.my/id/eprint/88004/
https://dx.doi.org/10.1016/j.enzmictec.2019.109367
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.utm.88004
record_format eprints
spelling my.utm.880042020-12-15T10:41:37Z http://eprints.utm.my/id/eprint/88004/ Effect of operative variables and kinetic study of butyl butyrate synthesis by candida rugosa lipase activated by chitosan-reinforced nanocellulose derived from raw oil palm leaves Elias, Nursyafiqah Abdul Wahab, Roswanira Chandren, Sheela Abdul Razak, Fazira Ilyana Jamalis, Joazaizulfazli QD Chemistry Currently, the chemically-assisted esterification to manufacture butyl butyrate employs corrosive homogeneous acid catalyst and liberates enormous quantities of hazardous by-products which complicate downstream treatment processes. This study aimed to identify the optimized esterification conditions, and the kinetic aspects of the enzyme-assisted synthesis of butyl butyrate using immobilized Candida rugosa lipase activated by chitosan-reinforced nanocellulose derived from raw oil palm leaves (CRL/CS-NC). The best process variables that gave the maximum conversion degree of butyl butyrate by CRL/CS-NC (90.2%) in just 3 h, as compared to free CRL (62.9%) are as follows: 50 °C, 1:2 M ratio of acid/alcohol, stirring rate of 200 rpm and a 3 mg/mL enzyme load. The enzymatic esterification followed the ping pong bi-bi mechanism with substrate inhibition, revealing a ˜1.1-fold higher Ki for CRL/CS-NC (55.55 mM) over free CRL (50.68 mM). This indicated that CRL/CS-NC was less inhibited by the substrates. Butanol was preferred over butyric acid as reflected by the higher apparent Michaelis-Menten constant of CRL/CS-NC for butanol (137 mM) than butyric acid (142.7 mM). Thus, the kinetics data conclusively showed that CRL/CS-NC (Vmax 0.48 mM min-1, Keff 0.07 min-1 mM-1) was catalytically more efficient than free CRL (Vmax 0.35 mM min-1, Keff 0.06 min-1 mM-1). Elsevier Inc. 2019-11 Article PeerReviewed Elias, Nursyafiqah and Abdul Wahab, Roswanira and Chandren, Sheela and Abdul Razak, Fazira Ilyana and Jamalis, Joazaizulfazli (2019) Effect of operative variables and kinetic study of butyl butyrate synthesis by candida rugosa lipase activated by chitosan-reinforced nanocellulose derived from raw oil palm leaves. Enzyme and Microbial Technology, 130 . p. 109367. ISSN 0141-0229 https://dx.doi.org/10.1016/j.enzmictec.2019.109367
institution Universiti Teknologi Malaysia
building UTM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Malaysia
content_source UTM Institutional Repository
url_provider http://eprints.utm.my/
topic QD Chemistry
spellingShingle QD Chemistry
Elias, Nursyafiqah
Abdul Wahab, Roswanira
Chandren, Sheela
Abdul Razak, Fazira Ilyana
Jamalis, Joazaizulfazli
Effect of operative variables and kinetic study of butyl butyrate synthesis by candida rugosa lipase activated by chitosan-reinforced nanocellulose derived from raw oil palm leaves
description Currently, the chemically-assisted esterification to manufacture butyl butyrate employs corrosive homogeneous acid catalyst and liberates enormous quantities of hazardous by-products which complicate downstream treatment processes. This study aimed to identify the optimized esterification conditions, and the kinetic aspects of the enzyme-assisted synthesis of butyl butyrate using immobilized Candida rugosa lipase activated by chitosan-reinforced nanocellulose derived from raw oil palm leaves (CRL/CS-NC). The best process variables that gave the maximum conversion degree of butyl butyrate by CRL/CS-NC (90.2%) in just 3 h, as compared to free CRL (62.9%) are as follows: 50 °C, 1:2 M ratio of acid/alcohol, stirring rate of 200 rpm and a 3 mg/mL enzyme load. The enzymatic esterification followed the ping pong bi-bi mechanism with substrate inhibition, revealing a ˜1.1-fold higher Ki for CRL/CS-NC (55.55 mM) over free CRL (50.68 mM). This indicated that CRL/CS-NC was less inhibited by the substrates. Butanol was preferred over butyric acid as reflected by the higher apparent Michaelis-Menten constant of CRL/CS-NC for butanol (137 mM) than butyric acid (142.7 mM). Thus, the kinetics data conclusively showed that CRL/CS-NC (Vmax 0.48 mM min-1, Keff 0.07 min-1 mM-1) was catalytically more efficient than free CRL (Vmax 0.35 mM min-1, Keff 0.06 min-1 mM-1).
format Article
author Elias, Nursyafiqah
Abdul Wahab, Roswanira
Chandren, Sheela
Abdul Razak, Fazira Ilyana
Jamalis, Joazaizulfazli
author_facet Elias, Nursyafiqah
Abdul Wahab, Roswanira
Chandren, Sheela
Abdul Razak, Fazira Ilyana
Jamalis, Joazaizulfazli
author_sort Elias, Nursyafiqah
title Effect of operative variables and kinetic study of butyl butyrate synthesis by candida rugosa lipase activated by chitosan-reinforced nanocellulose derived from raw oil palm leaves
title_short Effect of operative variables and kinetic study of butyl butyrate synthesis by candida rugosa lipase activated by chitosan-reinforced nanocellulose derived from raw oil palm leaves
title_full Effect of operative variables and kinetic study of butyl butyrate synthesis by candida rugosa lipase activated by chitosan-reinforced nanocellulose derived from raw oil palm leaves
title_fullStr Effect of operative variables and kinetic study of butyl butyrate synthesis by candida rugosa lipase activated by chitosan-reinforced nanocellulose derived from raw oil palm leaves
title_full_unstemmed Effect of operative variables and kinetic study of butyl butyrate synthesis by candida rugosa lipase activated by chitosan-reinforced nanocellulose derived from raw oil palm leaves
title_sort effect of operative variables and kinetic study of butyl butyrate synthesis by candida rugosa lipase activated by chitosan-reinforced nanocellulose derived from raw oil palm leaves
publisher Elsevier Inc.
publishDate 2019
url http://eprints.utm.my/id/eprint/88004/
https://dx.doi.org/10.1016/j.enzmictec.2019.109367
_version_ 1687393517177929728
score 13.211869

Similar Items