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Insights into microbial community structure and diversity in oil palm waste compost

Empty fruit bunch (EFB) and palm oil mill effluent (POME) are the major wastes generated by the oil palm industry in Malaysia. The practice of EFB and POME digester sludge co-composting has shown positive results, both in mitigating otherwise environmentally damaging waste streams and producing a us...

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Main Authors:	Abd. Khalid, Nurshafika, Rajandas, Heera, Parimannan, Sivachandran, Croft, Laurence J., Loke, Stella, Chong, Chun Shiong, Bruce, Neil C., Yahya, Adibah
Format:	Article
Published:	Springer Nature 2019
Subjects:	Q Science (General)
Online Access:	http://eprints.utm.my/id/eprint/87881/ http://dx.doi.org/10.1007/s13205-019-1892-4
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id	my.utm.87881
record_format	eprints
spelling	my.utm.878812020-11-30T13:29:12Z http://eprints.utm.my/id/eprint/87881/ Insights into microbial community structure and diversity in oil palm waste compost Abd. Khalid, Nurshafika Rajandas, Heera Parimannan, Sivachandran Croft, Laurence J. Loke, Stella Chong, Chun Shiong Bruce, Neil C. Yahya, Adibah Q Science (General) Empty fruit bunch (EFB) and palm oil mill effluent (POME) are the major wastes generated by the oil palm industry in Malaysia. The practice of EFB and POME digester sludge co-composting has shown positive results, both in mitigating otherwise environmentally damaging waste streams and producing a useful product (compost) from these streams. In this study, the bacterial ecosystems of 12-week-old EFB-POME co-compost and POME biogas sludge from Felda Maokil, Johor were analysed using 16S metagenome sequencing. Over ten phyla were detected, with Chloroflexi being the predominant phylum, representing approximately 53% of compost and 23% of the POME microbiome reads. The main bacterial lineage found in the compost and POME was Anaerolinaceae (Chloroflexi) with 30% and 18% of the total gene fragments, respectively. The significant differences between compost and POME communities were abundances of Syntrophobacter, Sulfuricurvum and Coprococcus. No methanogens were identified due to the bias in general 16S primers to eubacteria. The preponderance of anaerobic species in the compost and high abundance of secondary metabolite fermenting bacteria is due to an extended composting time, with anaerobic collapse of the pile due to the tropical heat. Predictive functional profiles of the metagenomes using 16S rRNA marker genes suggest that the presence of enzymes involved in degradation of polysaccharides such as glucoamylase, endoglucanase and arabinofuranosidase, all of which were strongly active in POME. Eubacterial species associated with cellulytic methanogenesis were present in both samples. Springer Nature 2019 Article PeerReviewed Abd. Khalid, Nurshafika and Rajandas, Heera and Parimannan, Sivachandran and Croft, Laurence J. and Loke, Stella and Chong, Chun Shiong and Bruce, Neil C. and Yahya, Adibah (2019) Insights into microbial community structure and diversity in oil palm waste compost. 3 Biotech, 9 (10). p. 364. ISSN 2190-5738 http://dx.doi.org/10.1007/s13205-019-1892-4
institution	Universiti Teknologi Malaysia
building	UTM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Teknologi Malaysia
content_source	UTM Institutional Repository
url_provider	http://eprints.utm.my/
topic	Q Science (General)
spellingShingle	Q Science (General) Abd. Khalid, Nurshafika Rajandas, Heera Parimannan, Sivachandran Croft, Laurence J. Loke, Stella Chong, Chun Shiong Bruce, Neil C. Yahya, Adibah Insights into microbial community structure and diversity in oil palm waste compost
description	Empty fruit bunch (EFB) and palm oil mill effluent (POME) are the major wastes generated by the oil palm industry in Malaysia. The practice of EFB and POME digester sludge co-composting has shown positive results, both in mitigating otherwise environmentally damaging waste streams and producing a useful product (compost) from these streams. In this study, the bacterial ecosystems of 12-week-old EFB-POME co-compost and POME biogas sludge from Felda Maokil, Johor were analysed using 16S metagenome sequencing. Over ten phyla were detected, with Chloroflexi being the predominant phylum, representing approximately 53% of compost and 23% of the POME microbiome reads. The main bacterial lineage found in the compost and POME was Anaerolinaceae (Chloroflexi) with 30% and 18% of the total gene fragments, respectively. The significant differences between compost and POME communities were abundances of Syntrophobacter, Sulfuricurvum and Coprococcus. No methanogens were identified due to the bias in general 16S primers to eubacteria. The preponderance of anaerobic species in the compost and high abundance of secondary metabolite fermenting bacteria is due to an extended composting time, with anaerobic collapse of the pile due to the tropical heat. Predictive functional profiles of the metagenomes using 16S rRNA marker genes suggest that the presence of enzymes involved in degradation of polysaccharides such as glucoamylase, endoglucanase and arabinofuranosidase, all of which were strongly active in POME. Eubacterial species associated with cellulytic methanogenesis were present in both samples.
format	Article
author	Abd. Khalid, Nurshafika Rajandas, Heera Parimannan, Sivachandran Croft, Laurence J. Loke, Stella Chong, Chun Shiong Bruce, Neil C. Yahya, Adibah
author_facet	Abd. Khalid, Nurshafika Rajandas, Heera Parimannan, Sivachandran Croft, Laurence J. Loke, Stella Chong, Chun Shiong Bruce, Neil C. Yahya, Adibah
author_sort	Abd. Khalid, Nurshafika
title	Insights into microbial community structure and diversity in oil palm waste compost
title_short	Insights into microbial community structure and diversity in oil palm waste compost
title_full	Insights into microbial community structure and diversity in oil palm waste compost
title_fullStr	Insights into microbial community structure and diversity in oil palm waste compost
title_full_unstemmed	Insights into microbial community structure and diversity in oil palm waste compost
title_sort	insights into microbial community structure and diversity in oil palm waste compost
publisher	Springer Nature
publishDate	2019
url	http://eprints.utm.my/id/eprint/87881/ http://dx.doi.org/10.1007/s13205-019-1892-4
_version_	1685579003556003840
score	13.211869

Insights into microbial community structure and diversity in oil palm waste compost

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