Staff View: Optimization of the water extraction process on total phenolic content from Labisia pumila

Optimization of the water extraction process on total phenolic content from Labisia pumila

Labisia pumila is one of the most widely used medicinal herbs among women in Southeast Asia.There is an increasing demand force for this herb in pharmaceutical and food industries. Most of these products are registered without knowing the level of bioactivity in the extracts and not in standardised...

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Main Authors:	Faidzul Adzhar, Nurul Fazila, Mohamad Azam, Zaheda, Musa, Nur Fashya, Abd. Latiff, Norliza, Yaakub, Harisun, Nadri, Muhammad Helmi, Othman, Nor Zalina
Format:	Article
Published:	Penerbit UTM Press 2019
Subjects:	QK Botany
Online Access:	http://eprints.utm.my/id/eprint/87574/ http://dx.doi.org/10.11113/mjfas.v15n5.1326
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spelling	my.utm.875742020-11-30T09:04:00Z http://eprints.utm.my/id/eprint/87574/ Optimization of the water extraction process on total phenolic content from Labisia pumila Faidzul Adzhar, Nurul Fazila Mohamad Azam, Zaheda Musa, Nur Fashya Abd. Latiff, Norliza Yaakub, Harisun Nadri, Muhammad Helmi Othman, Nor Zalina QK Botany Labisia pumila is one of the most widely used medicinal herbs among women in Southeast Asia.There is an increasing demand force for this herb in pharmaceutical and food industries. Most of these products are registered without knowing the level of bioactivity in the extracts and not in standardised form. Non-standardized extract is perceived as a low-quality herbal product, hence lowering its market value. Therefore, this study aimed to standardizethe optimal water extraction conditions for maximum total phenolic content (TPC) of L. pumila. In this study, Response Surface Methodology (RSM) was used to optimize the extraction process of TPC from L. pumila. Dried whole plant of L. pumila was extracted in water as solvent at different temperatures, times, and solid to water ratios that have been identified to be significantly affecting the recovery of TPC. A Box-Behnken design was used to investigate the effects of three independent variables that were coded at three levels consisted of 30 experimental points using decoction method. A second-order polynomial model was used for predicting the response. Regression analysis showed that more than 91.99 % of the variation was explained by the models. Results identified temperature as the most significant (p<0.05) factor affecting the TPC. The optimal conditions obtained from RSM were 60 ᴼC for the temperature, 2.67 hours for the extraction time and 1:10 for the solid to water ratio. Under these optimal conditions, the response value of the experimental values agreed with the predicted value of TPC. In conclusion, the present study has succesfully standardized optimal temperature, time and solid-water ratio of L. pumila water extraction process for high TPC Penerbit UTM Press 2019 Article PeerReviewed Faidzul Adzhar, Nurul Fazila and Mohamad Azam, Zaheda and Musa, Nur Fashya and Abd. Latiff, Norliza and Yaakub, Harisun and Nadri, Muhammad Helmi and Othman, Nor Zalina (2019) Optimization of the water extraction process on total phenolic content from Labisia pumila. Malaysian Journal Of Fundamental And Applied Sciences, 15 (5). pp. 739-742. ISSN 2288-5981 http://dx.doi.org/10.11113/mjfas.v15n5.1326
institution	Universiti Teknologi Malaysia
building	UTM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Teknologi Malaysia
content_source	UTM Institutional Repository
url_provider	http://eprints.utm.my/
topic	QK Botany
spellingShingle	QK Botany Faidzul Adzhar, Nurul Fazila Mohamad Azam, Zaheda Musa, Nur Fashya Abd. Latiff, Norliza Yaakub, Harisun Nadri, Muhammad Helmi Othman, Nor Zalina Optimization of the water extraction process on total phenolic content from Labisia pumila
description	Labisia pumila is one of the most widely used medicinal herbs among women in Southeast Asia.There is an increasing demand force for this herb in pharmaceutical and food industries. Most of these products are registered without knowing the level of bioactivity in the extracts and not in standardised form. Non-standardized extract is perceived as a low-quality herbal product, hence lowering its market value. Therefore, this study aimed to standardizethe optimal water extraction conditions for maximum total phenolic content (TPC) of L. pumila. In this study, Response Surface Methodology (RSM) was used to optimize the extraction process of TPC from L. pumila. Dried whole plant of L. pumila was extracted in water as solvent at different temperatures, times, and solid to water ratios that have been identified to be significantly affecting the recovery of TPC. A Box-Behnken design was used to investigate the effects of three independent variables that were coded at three levels consisted of 30 experimental points using decoction method. A second-order polynomial model was used for predicting the response. Regression analysis showed that more than 91.99 % of the variation was explained by the models. Results identified temperature as the most significant (p<0.05) factor affecting the TPC. The optimal conditions obtained from RSM were 60 ᴼC for the temperature, 2.67 hours for the extraction time and 1:10 for the solid to water ratio. Under these optimal conditions, the response value of the experimental values agreed with the predicted value of TPC. In conclusion, the present study has succesfully standardized optimal temperature, time and solid-water ratio of L. pumila water extraction process for high TPC
format	Article
author	Faidzul Adzhar, Nurul Fazila Mohamad Azam, Zaheda Musa, Nur Fashya Abd. Latiff, Norliza Yaakub, Harisun Nadri, Muhammad Helmi Othman, Nor Zalina
author_facet	Faidzul Adzhar, Nurul Fazila Mohamad Azam, Zaheda Musa, Nur Fashya Abd. Latiff, Norliza Yaakub, Harisun Nadri, Muhammad Helmi Othman, Nor Zalina
author_sort	Faidzul Adzhar, Nurul Fazila
title	Optimization of the water extraction process on total phenolic content from Labisia pumila
title_short	Optimization of the water extraction process on total phenolic content from Labisia pumila
title_full	Optimization of the water extraction process on total phenolic content from Labisia pumila
title_fullStr	Optimization of the water extraction process on total phenolic content from Labisia pumila
title_full_unstemmed	Optimization of the water extraction process on total phenolic content from Labisia pumila
title_sort	optimization of the water extraction process on total phenolic content from labisia pumila
publisher	Penerbit UTM Press
publishDate	2019
url	http://eprints.utm.my/id/eprint/87574/ http://dx.doi.org/10.11113/mjfas.v15n5.1326
_version_	1685578956938412032
score	13.211869

Optimization of the water extraction process on total phenolic content from Labisia pumila

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