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Survival of antibiotic resistant escherichia coli in vacuum-packed keropok lekor: food safety alert among sme keropok lekor producers

The microbiological quality of thirty ready-to-eat (RTE) keropok lekor (a sausage shape Malaysian fish product) was evaluated in comparison to microbiological guidelines for ready to eat foods. The two E. coli isolates were subjected to DNA sequencing, identified and tested for their resistance towa...

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Main Authors: Wan Hamat, H., Lani, M. N., Hamzah, Y., Alias, R., Hassan, Z., Mahat, N. A.
Format: Article
Language:English
Published: Malaysian Society for Parasitology 2020
Subjects:
TP Chemical technology
Online Access:http://eprints.utm.my/id/eprint/87377/1/MohdNizamLani2020_SurvivalofAntibioticResistantEscherichiaColi.pdf
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spelling my.utm.873772020-11-08T03:55:46Z http://eprints.utm.my/id/eprint/87377/ Survival of antibiotic resistant escherichia coli in vacuum-packed keropok lekor: food safety alert among sme keropok lekor producers Wan Hamat, H. Lani, M. N. Hamzah, Y. Alias, R. Hassan, Z. Mahat, N. A. TP Chemical technology The microbiological quality of thirty ready-to-eat (RTE) keropok lekor (a sausage shape Malaysian fish product) was evaluated in comparison to microbiological guidelines for ready to eat foods. The two E. coli isolates were subjected to DNA sequencing, identified and tested for their resistance towards fifteen different antibiotics. The survival and growth of the isolated E. coli strains inoculated in keropok lekor at atmospheric air and vacuum packaging were also evaluated. Results revealed that four samples (13.33%) contained Enterobacteriaceae counts that exceeded the recommended allowable counts of 4.0 log10 CFU/g. Unsatisfactory level of coliforms (> 1.7 log10 CFU/g) was also observed in ten of the samples; two of which contained E. coli (2.1 ± 0.17 and 3.7 ± 0.02 log10 CFU/g), suggesting of poor hygiene and sanitation practices. While the ‘Possible E10’ E. coli strain was observably resistant towards Nalidixic acid (30µg) alone, B10 E. coli isolate was worryingly resistant towards Ampicillin (10µg), Ceftazidime (30µg), Ciprofloxacin (5µg), Ceftriaxone (30µg), Nalidixic acid (30µg) and Tetracycline (30µg). This study also revealed that the growth and survival of the ‘Possible E10’ and B10 E. coli strains were not significantly affected by vacuum packaging when stored at both 4°C and 28°C. Therefore, intervention programmes to alert and educate small-medium enterprisers (SMEs) of keropok lekor producers on food safety as well as potential health risks that can be associated due to inappropriate handling procedures of such product, merits consideration. Malaysian Society for Parasitology 2020-03 Article PeerReviewed application/pdf en http://eprints.utm.my/id/eprint/87377/1/MohdNizamLani2020_SurvivalofAntibioticResistantEscherichiaColi.pdf Wan Hamat, H. and Lani, M. N. and Hamzah, Y. and Alias, R. and Hassan, Z. and Mahat, N. A. (2020) Survival of antibiotic resistant escherichia coli in vacuum-packed keropok lekor: food safety alert among sme keropok lekor producers. Tropical Biomedicine, 37 (1). pp. 103-115. ISSN 0127-5720 https://www-scopus-com.ezproxy.utm.my/record/display.uri?eid=2-s2.0-85088066577&origin=resultslist&sort=plf-f&src=s&st1=
institution Universiti Teknologi Malaysia
building UTM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Malaysia
content_source UTM Institutional Repository
url_provider http://eprints.utm.my/
language English
topic TP Chemical technology
spellingShingle TP Chemical technology
Wan Hamat, H.
Lani, M. N.
Hamzah, Y.
Alias, R.
Hassan, Z.
Mahat, N. A.
Survival of antibiotic resistant escherichia coli in vacuum-packed keropok lekor: food safety alert among sme keropok lekor producers
description The microbiological quality of thirty ready-to-eat (RTE) keropok lekor (a sausage shape Malaysian fish product) was evaluated in comparison to microbiological guidelines for ready to eat foods. The two E. coli isolates were subjected to DNA sequencing, identified and tested for their resistance towards fifteen different antibiotics. The survival and growth of the isolated E. coli strains inoculated in keropok lekor at atmospheric air and vacuum packaging were also evaluated. Results revealed that four samples (13.33%) contained Enterobacteriaceae counts that exceeded the recommended allowable counts of 4.0 log10 CFU/g. Unsatisfactory level of coliforms (> 1.7 log10 CFU/g) was also observed in ten of the samples; two of which contained E. coli (2.1 ± 0.17 and 3.7 ± 0.02 log10 CFU/g), suggesting of poor hygiene and sanitation practices. While the ‘Possible E10’ E. coli strain was observably resistant towards Nalidixic acid (30µg) alone, B10 E. coli isolate was worryingly resistant towards Ampicillin (10µg), Ceftazidime (30µg), Ciprofloxacin (5µg), Ceftriaxone (30µg), Nalidixic acid (30µg) and Tetracycline (30µg). This study also revealed that the growth and survival of the ‘Possible E10’ and B10 E. coli strains were not significantly affected by vacuum packaging when stored at both 4°C and 28°C. Therefore, intervention programmes to alert and educate small-medium enterprisers (SMEs) of keropok lekor producers on food safety as well as potential health risks that can be associated due to inappropriate handling procedures of such product, merits consideration.
format Article
author Wan Hamat, H.
Lani, M. N.
Hamzah, Y.
Alias, R.
Hassan, Z.
Mahat, N. A.
author_facet Wan Hamat, H.
Lani, M. N.
Hamzah, Y.
Alias, R.
Hassan, Z.
Mahat, N. A.
author_sort Wan Hamat, H.
title Survival of antibiotic resistant escherichia coli in vacuum-packed keropok lekor: food safety alert among sme keropok lekor producers
title_short Survival of antibiotic resistant escherichia coli in vacuum-packed keropok lekor: food safety alert among sme keropok lekor producers
title_full Survival of antibiotic resistant escherichia coli in vacuum-packed keropok lekor: food safety alert among sme keropok lekor producers
title_fullStr Survival of antibiotic resistant escherichia coli in vacuum-packed keropok lekor: food safety alert among sme keropok lekor producers
title_full_unstemmed Survival of antibiotic resistant escherichia coli in vacuum-packed keropok lekor: food safety alert among sme keropok lekor producers
title_sort survival of antibiotic resistant escherichia coli in vacuum-packed keropok lekor: food safety alert among sme keropok lekor producers
publisher Malaysian Society for Parasitology
publishDate 2020
url http://eprints.utm.my/id/eprint/87377/1/MohdNizamLani2020_SurvivalofAntibioticResistantEscherichiaColi.pdf
http://eprints.utm.my/id/eprint/87377/
https://www-scopus-com.ezproxy.utm.my/record/display.uri?eid=2-s2.0-85088066577&origin=resultslist&sort=plf-f&src=s&st1=
_version_ 1683230759344668672
score 13.211869

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