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Development of the PVA/CS nanofibers containing silk protein sericin as a wound dressing: In vitro and in vivo assessment

Skin and soft tissue infections are major concerns with respect to wound repair. Recently, anti-bacterial wound dressings have been emerging as promising candidates to reduce infection, thus accelerating the wound healing process. This paper presents our work to develop and characterize poly(vinyl a...

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Main Authors: Bakhsheshi-Rad, Hamid Reza, Ismail, Ahmad Fauzi, Aziz, Madzlan, Akbari, Mohsen, Hadisi, Zhina, Omidi, Mahdi, Chen, Xiongbiao
Format: Article
Published: Elsevier B. V. 2020
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TP Chemical technology
Online Access:http://eprints.utm.my/id/eprint/87238/
http://dx.doi.org/10.1016/j.ijbiomac.2020.01.139
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spelling my.utm.872382020-12-01T07:45:13Z http://eprints.utm.my/id/eprint/87238/ Development of the PVA/CS nanofibers containing silk protein sericin as a wound dressing: In vitro and in vivo assessment Bakhsheshi-Rad, Hamid Reza Ismail, Ahmad Fauzi Aziz, Madzlan Akbari, Mohsen Hadisi, Zhina Omidi, Mahdi Chen, Xiongbiao TP Chemical technology Skin and soft tissue infections are major concerns with respect to wound repair. Recently, anti-bacterial wound dressings have been emerging as promising candidates to reduce infection, thus accelerating the wound healing process. This paper presents our work to develop and characterize poly(vinyl alcohol) (PVA)/chitosan (CS)/silk sericin (SS)/tetracycline (TCN) porous nanofibers, with diameters varying from 305 to 425 nm, both in vitro and in vivo for potential applications as wound dressings. The fabricated nanofibers possess a considerable capacity to take up water through swelling (~325–650%). Sericin addition leads to increased hydrophilicity and elongation at break while decreasing fiber diameter and mechanical strength. Moreover, fibroblasts (L929) cultured on the nanofibers with low sericin content (PVA/CS/1-2SS) displayed greater proliferation compared to those on nanofibers without sericin (PVA/CS). Nanofibers loaded with high sericin and tetracycline content significantly inhibited the growth of Escherichia coli and Staphylococcus aureus. In vivo examination revealed that PVA/CS/2SS-TCN nanofibers enhance wound healing, re-epithelialization, and collagen deposition compared to traditional gauze and nanofibers without sericin. The results of this study demonstrate that the PVA/CS/2SS-TCN nanofiber creates a promising alternative to traditional wound dressing materials. Elsevier B. V. 2020 Article PeerReviewed Bakhsheshi-Rad, Hamid Reza and Ismail, Ahmad Fauzi and Aziz, Madzlan and Akbari, Mohsen and Hadisi, Zhina and Omidi, Mahdi and Chen, Xiongbiao (2020) Development of the PVA/CS nanofibers containing silk protein sericin as a wound dressing: In vitro and in vivo assessment. International Journal of Biological Macromolecules, 149 . pp. 513-521. ISSN 0141-8130 http://dx.doi.org/10.1016/j.ijbiomac.2020.01.139
institution Universiti Teknologi Malaysia
building UTM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Malaysia
content_source UTM Institutional Repository
url_provider http://eprints.utm.my/
topic TP Chemical technology
spellingShingle TP Chemical technology
Bakhsheshi-Rad, Hamid Reza
Ismail, Ahmad Fauzi
Aziz, Madzlan
Akbari, Mohsen
Hadisi, Zhina
Omidi, Mahdi
Chen, Xiongbiao
Development of the PVA/CS nanofibers containing silk protein sericin as a wound dressing: In vitro and in vivo assessment
description Skin and soft tissue infections are major concerns with respect to wound repair. Recently, anti-bacterial wound dressings have been emerging as promising candidates to reduce infection, thus accelerating the wound healing process. This paper presents our work to develop and characterize poly(vinyl alcohol) (PVA)/chitosan (CS)/silk sericin (SS)/tetracycline (TCN) porous nanofibers, with diameters varying from 305 to 425 nm, both in vitro and in vivo for potential applications as wound dressings. The fabricated nanofibers possess a considerable capacity to take up water through swelling (~325–650%). Sericin addition leads to increased hydrophilicity and elongation at break while decreasing fiber diameter and mechanical strength. Moreover, fibroblasts (L929) cultured on the nanofibers with low sericin content (PVA/CS/1-2SS) displayed greater proliferation compared to those on nanofibers without sericin (PVA/CS). Nanofibers loaded with high sericin and tetracycline content significantly inhibited the growth of Escherichia coli and Staphylococcus aureus. In vivo examination revealed that PVA/CS/2SS-TCN nanofibers enhance wound healing, re-epithelialization, and collagen deposition compared to traditional gauze and nanofibers without sericin. The results of this study demonstrate that the PVA/CS/2SS-TCN nanofiber creates a promising alternative to traditional wound dressing materials.
format Article
author Bakhsheshi-Rad, Hamid Reza
Ismail, Ahmad Fauzi
Aziz, Madzlan
Akbari, Mohsen
Hadisi, Zhina
Omidi, Mahdi
Chen, Xiongbiao
author_facet Bakhsheshi-Rad, Hamid Reza
Ismail, Ahmad Fauzi
Aziz, Madzlan
Akbari, Mohsen
Hadisi, Zhina
Omidi, Mahdi
Chen, Xiongbiao
author_sort Bakhsheshi-Rad, Hamid Reza
title Development of the PVA/CS nanofibers containing silk protein sericin as a wound dressing: In vitro and in vivo assessment
title_short Development of the PVA/CS nanofibers containing silk protein sericin as a wound dressing: In vitro and in vivo assessment
title_full Development of the PVA/CS nanofibers containing silk protein sericin as a wound dressing: In vitro and in vivo assessment
title_fullStr Development of the PVA/CS nanofibers containing silk protein sericin as a wound dressing: In vitro and in vivo assessment
title_full_unstemmed Development of the PVA/CS nanofibers containing silk protein sericin as a wound dressing: In vitro and in vivo assessment
title_sort development of the pva/cs nanofibers containing silk protein sericin as a wound dressing: in vitro and in vivo assessment
publisher Elsevier B. V.
publishDate 2020
url http://eprints.utm.my/id/eprint/87238/
http://dx.doi.org/10.1016/j.ijbiomac.2020.01.139
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score 13.211869

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