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Tree bark scrape fungus: a potential source of laccase for application in bioremediation of non-textile dyes

Although laccase has been recognized as a wonder molecule and green enzyme, the use of low yielding fungal strains, poor production, purification, and low enzyme kinetics have hampered its large-scale application. Thus,this study aims to select high yielding fungal strains and optimize the productio...

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Main Authors:	Sayyed, R. Z., Bhamare, H. M., Sapna, Sapna, Marraiki, N., Elgorban, A. M., Syed, A., El-Enshasy, H. A., Dailin, D. J.
Format:	Article
Language:	English
Published:	Public Library of Science 2020
Subjects:	TP Chemical technology
Online Access:	http://eprints.utm.my/id/eprint/86994/1/RZSayyed2020_TreeBarkScrapeFungus.pdf http://eprints.utm.my/id/eprint/86994/ http://www.dx.doi.org/10.1371/journal.pone.0229968
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spelling	my.utm.869942020-10-25T07:25:59Z http://eprints.utm.my/id/eprint/86994/ Tree bark scrape fungus: a potential source of laccase for application in bioremediation of non-textile dyes Sayyed, R. Z. Bhamare, H. M. Sapna, Sapna Marraiki, N. Elgorban, A. M. Syed, A. El-Enshasy, H. A. Dailin, D. J. TP Chemical technology Although laccase has been recognized as a wonder molecule and green enzyme, the use of low yielding fungal strains, poor production, purification, and low enzyme kinetics have hampered its large-scale application. Thus,this study aims to select high yielding fungal strains and optimize the production, purification, and kinetics of laccase of Aspergillus sp. HB-RZ4. The results obtained indicated that Aspergillus sp. HB-RZ4 produced a significantly large amount of laccase under meso-acidophilic shaking conditions in a medium containing glucose and yeast extract. A 25 μM CuSO4 was observed to enhance the enzyme yield. The enzyme was best purified on a Sephadex G-100 column. The purified enzyme resembled laccase of A. flavus. The kinetics of the purified enzyme revealed high substrate specificity and good velocity of reaction,using ABTS as a substrate. The enzyme was observed to be stable over various pH values and temperatures. The peptide structure of the purified enzyme was found to resemble laccase of A. kawachii IFO 4308. The fungus was observed to decolorize various dyes independent of the requirement of a laccase mediator system. Aspergillus sp. HB-RZ4 was observed to be a potent natural producer of laccase, and it decolorized the dyes even in the absence of a laccase mediator system. Thus, it can be used for bioremediation of effluent that contains non-textile dyes. © 2020 Sayyed et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Public Library of Science 2020-06 Article PeerReviewed application/pdf en http://eprints.utm.my/id/eprint/86994/1/RZSayyed2020_TreeBarkScrapeFungus.pdf Sayyed, R. Z. and Bhamare, H. M. and Sapna, Sapna and Marraiki, N. and Elgorban, A. M. and Syed, A. and El-Enshasy, H. A. and Dailin, D. J. (2020) Tree bark scrape fungus: a potential source of laccase for application in bioremediation of non-textile dyes. PLoS ONE, 15 (6). ISSN 1932-6203 http://www.dx.doi.org/10.1371/journal.pone.0229968 DOI:10.1371/journal.pone.0229968
institution	Universiti Teknologi Malaysia
building	UTM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Teknologi Malaysia
content_source	UTM Institutional Repository
url_provider	http://eprints.utm.my/
language	English
topic	TP Chemical technology
spellingShingle	TP Chemical technology Sayyed, R. Z. Bhamare, H. M. Sapna, Sapna Marraiki, N. Elgorban, A. M. Syed, A. El-Enshasy, H. A. Dailin, D. J. Tree bark scrape fungus: a potential source of laccase for application in bioremediation of non-textile dyes
description	Although laccase has been recognized as a wonder molecule and green enzyme, the use of low yielding fungal strains, poor production, purification, and low enzyme kinetics have hampered its large-scale application. Thus,this study aims to select high yielding fungal strains and optimize the production, purification, and kinetics of laccase of Aspergillus sp. HB-RZ4. The results obtained indicated that Aspergillus sp. HB-RZ4 produced a significantly large amount of laccase under meso-acidophilic shaking conditions in a medium containing glucose and yeast extract. A 25 μM CuSO4 was observed to enhance the enzyme yield. The enzyme was best purified on a Sephadex G-100 column. The purified enzyme resembled laccase of A. flavus. The kinetics of the purified enzyme revealed high substrate specificity and good velocity of reaction,using ABTS as a substrate. The enzyme was observed to be stable over various pH values and temperatures. The peptide structure of the purified enzyme was found to resemble laccase of A. kawachii IFO 4308. The fungus was observed to decolorize various dyes independent of the requirement of a laccase mediator system. Aspergillus sp. HB-RZ4 was observed to be a potent natural producer of laccase, and it decolorized the dyes even in the absence of a laccase mediator system. Thus, it can be used for bioremediation of effluent that contains non-textile dyes. © 2020 Sayyed et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
format	Article
author	Sayyed, R. Z. Bhamare, H. M. Sapna, Sapna Marraiki, N. Elgorban, A. M. Syed, A. El-Enshasy, H. A. Dailin, D. J.
author_facet	Sayyed, R. Z. Bhamare, H. M. Sapna, Sapna Marraiki, N. Elgorban, A. M. Syed, A. El-Enshasy, H. A. Dailin, D. J.
author_sort	Sayyed, R. Z.
title	Tree bark scrape fungus: a potential source of laccase for application in bioremediation of non-textile dyes
title_short	Tree bark scrape fungus: a potential source of laccase for application in bioremediation of non-textile dyes
title_full	Tree bark scrape fungus: a potential source of laccase for application in bioremediation of non-textile dyes
title_fullStr	Tree bark scrape fungus: a potential source of laccase for application in bioremediation of non-textile dyes
title_full_unstemmed	Tree bark scrape fungus: a potential source of laccase for application in bioremediation of non-textile dyes
title_sort	tree bark scrape fungus: a potential source of laccase for application in bioremediation of non-textile dyes
publisher	Public Library of Science
publishDate	2020
url	http://eprints.utm.my/id/eprint/86994/1/RZSayyed2020_TreeBarkScrapeFungus.pdf http://eprints.utm.my/id/eprint/86994/ http://www.dx.doi.org/10.1371/journal.pone.0229968
_version_	1683230690721660928
score	13.211869

Tree bark scrape fungus: a potential source of laccase for application in bioremediation of non-textile dyes

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