Staff View: In vitro degradation behavior, antibacterial activity and cytotoxicity of TiO2-MAO/ZnHA composite coating on Mg alloy for orthopedic implants

In vitro degradation behavior, antibacterial activity and cytotoxicity of TiO2-MAO/ZnHA composite coating on Mg alloy for orthopedic implants

Magnesium alloys as biodegradable materials have received great attention for orthopedic application as a result of their good biocompatibility, bioactivity, and mechanical properties. However, the clinical use of Mg alloys is restricted by high degradation rate. In order to reduce the degradation r...

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Main Authors:	Bakhsheshi-Rad, H. R., Hamzah, E., Ismail, A. F., Aziz, M., Daroonparvar, M., Saebnoori, E., Chamia, A.
Format:	Article
Published:	Elsevier B. V. 2018
Subjects:	TJ Mechanical engineering and machinery
Online Access:	http://eprints.utm.my/id/eprint/86709/ http://dx.doi.org/10.1016/j.surfcoat.2017.11.027
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id	my.utm.86709
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spelling	my.utm.867092020-09-30T09:04:52Z http://eprints.utm.my/id/eprint/86709/ In vitro degradation behavior, antibacterial activity and cytotoxicity of TiO2-MAO/ZnHA composite coating on Mg alloy for orthopedic implants Bakhsheshi-Rad, H. R. Hamzah, E. Ismail, A. F. Aziz, M. Daroonparvar, M. Saebnoori, E. Chamia, A. TJ Mechanical engineering and machinery Magnesium alloys as biodegradable materials have received great attention for orthopedic application as a result of their good biocompatibility, bioactivity, and mechanical properties. However, the clinical use of Mg alloys is restricted by high degradation rate. In order to reduce the degradation rate, TiO2 incorporated micro-arc oxidation (TM) coatings were prepared on Mg- Ca alloy using micro-arc oxidation (MAO). Subsequently, zinc-doped hydroxyapatite (ZH) coating was deposited by electrophoretic deposition (EPD) on the MAO coating. The electrochemical test results demonstrated that the deposition of ZH composite coatings on Mg alloy significantly reduces its corrosion rate and improves its charge transfer resistance. Antibacterial activity of the coating against Escherichia coli (E. coli) was studied using disk-diffusion and spread plate methods. The results revealed that the inhibition zone amplified after deposition of TM and ZH coatings on Mg alloy, whereas more inhibition zone was found around ZH coating. In addition, the number of E. coli colonies reduces to 92% after ZH coating implying its good antibacterial properties. The cytotoxicity test indicated that cell viability of MG63 osteoblast cells cultured with ZH extracts was higher compared to the TM coating and bare Mg alloy. These results confirm that Mg alloy coated by TM/ZH exhibits high corrosion resistance, antibacterial activity and favorable bioactivity and cytocompatibility, indicating their substantial potentials for biomedical applications. Elsevier B. V. 2018-01 Article PeerReviewed Bakhsheshi-Rad, H. R. and Hamzah, E. and Ismail, A. F. and Aziz, M. and Daroonparvar, M. and Saebnoori, E. and Chamia, A. (2018) In vitro degradation behavior, antibacterial activity and cytotoxicity of TiO2-MAO/ZnHA composite coating on Mg alloy for orthopedic implants. Surface and Coatings Technology, 334 . pp. 450-460. ISSN 0257-8972 http://dx.doi.org/10.1016/j.surfcoat.2017.11.027
institution	Universiti Teknologi Malaysia
building	UTM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Teknologi Malaysia
content_source	UTM Institutional Repository
url_provider	http://eprints.utm.my/
topic	TJ Mechanical engineering and machinery
spellingShingle	TJ Mechanical engineering and machinery Bakhsheshi-Rad, H. R. Hamzah, E. Ismail, A. F. Aziz, M. Daroonparvar, M. Saebnoori, E. Chamia, A. In vitro degradation behavior, antibacterial activity and cytotoxicity of TiO2-MAO/ZnHA composite coating on Mg alloy for orthopedic implants
description	Magnesium alloys as biodegradable materials have received great attention for orthopedic application as a result of their good biocompatibility, bioactivity, and mechanical properties. However, the clinical use of Mg alloys is restricted by high degradation rate. In order to reduce the degradation rate, TiO2 incorporated micro-arc oxidation (TM) coatings were prepared on Mg- Ca alloy using micro-arc oxidation (MAO). Subsequently, zinc-doped hydroxyapatite (ZH) coating was deposited by electrophoretic deposition (EPD) on the MAO coating. The electrochemical test results demonstrated that the deposition of ZH composite coatings on Mg alloy significantly reduces its corrosion rate and improves its charge transfer resistance. Antibacterial activity of the coating against Escherichia coli (E. coli) was studied using disk-diffusion and spread plate methods. The results revealed that the inhibition zone amplified after deposition of TM and ZH coatings on Mg alloy, whereas more inhibition zone was found around ZH coating. In addition, the number of E. coli colonies reduces to 92% after ZH coating implying its good antibacterial properties. The cytotoxicity test indicated that cell viability of MG63 osteoblast cells cultured with ZH extracts was higher compared to the TM coating and bare Mg alloy. These results confirm that Mg alloy coated by TM/ZH exhibits high corrosion resistance, antibacterial activity and favorable bioactivity and cytocompatibility, indicating their substantial potentials for biomedical applications.
format	Article
author	Bakhsheshi-Rad, H. R. Hamzah, E. Ismail, A. F. Aziz, M. Daroonparvar, M. Saebnoori, E. Chamia, A.
author_facet	Bakhsheshi-Rad, H. R. Hamzah, E. Ismail, A. F. Aziz, M. Daroonparvar, M. Saebnoori, E. Chamia, A.
author_sort	Bakhsheshi-Rad, H. R.
title	In vitro degradation behavior, antibacterial activity and cytotoxicity of TiO2-MAO/ZnHA composite coating on Mg alloy for orthopedic implants
title_short	In vitro degradation behavior, antibacterial activity and cytotoxicity of TiO2-MAO/ZnHA composite coating on Mg alloy for orthopedic implants
title_full	In vitro degradation behavior, antibacterial activity and cytotoxicity of TiO2-MAO/ZnHA composite coating on Mg alloy for orthopedic implants
title_fullStr	In vitro degradation behavior, antibacterial activity and cytotoxicity of TiO2-MAO/ZnHA composite coating on Mg alloy for orthopedic implants
title_full_unstemmed	In vitro degradation behavior, antibacterial activity and cytotoxicity of TiO2-MAO/ZnHA composite coating on Mg alloy for orthopedic implants
title_sort	in vitro degradation behavior, antibacterial activity and cytotoxicity of tio2-mao/znha composite coating on mg alloy for orthopedic implants
publisher	Elsevier B. V.
publishDate	2018
url	http://eprints.utm.my/id/eprint/86709/ http://dx.doi.org/10.1016/j.surfcoat.2017.11.027
_version_	1680321084689219584
score	13.211869

In vitro degradation behavior, antibacterial activity and cytotoxicity of TiO2-MAO/ZnHA composite coating on Mg alloy for orthopedic implants

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