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Comparison of DNA extraction methods for entomological origin identification of honey using simple additive weighting method

Four different DNA protocols were compared to obtain the best method for DNA extraction of bees in honey samples. The efficiency was evaluated in terms of DNA concentration and purity, PCR amplification capacity targeting mitochondrial 16S rRNA gene, and execution time. The most efficient DNA extrac...

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Main Authors:	Kek, Siok Peng, Chin, Nyuk Ling, Tan, Sheau Wei, Yusof, Yus Aniza, Chua, Lee Suan
Format:	Article
Published:	Blackwell Publishing Ltd 2018
Subjects:	TP Chemical technology
Online Access:	http://eprints.utm.my/id/eprint/86688/ http://dx.doi.org/10.1111/ijfs.13840
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id	my.utm.86688
record_format	eprints
spelling	my.utm.866882020-09-30T09:04:40Z http://eprints.utm.my/id/eprint/86688/ Comparison of DNA extraction methods for entomological origin identification of honey using simple additive weighting method Kek, Siok Peng Chin, Nyuk Ling Tan, Sheau Wei Yusof, Yus Aniza Chua, Lee Suan TP Chemical technology Four different DNA protocols were compared to obtain the best method for DNA extraction of bees in honey samples. The efficiency was evaluated in terms of DNA concentration and purity, PCR amplification capacity targeting mitochondrial 16S rRNA gene, and execution time. The most efficient DNA extraction method was selected using simple additive weighting (SAW) analysis. The DNeasy method with silica membrane spin-column format scored highest in the final ranking of SAW compared to CTAB-based, Wizard and NucleoSpin methods. It yielded extracted DNA with sufficient concentration (second highest), acceptable purity (1.5–2.0), amplifiable for 16S rRNA gene region, and required least extraction time. This recommended DNA extraction protocol suggests that genomic methodologies using a 150 bp amplicon of the 16S rRNA gene of bees enables identification of the entomological origin of honey. Blackwell Publishing Ltd 2018-11 Article PeerReviewed Kek, Siok Peng and Chin, Nyuk Ling and Tan, Sheau Wei and Yusof, Yus Aniza and Chua, Lee Suan (2018) Comparison of DNA extraction methods for entomological origin identification of honey using simple additive weighting method. International Journal of Food Science and Technology, 53 (11). pp. 2490-2499. ISSN 0950-5423 http://dx.doi.org/10.1111/ijfs.13840 DOI:10.1111/ijfs.13840
institution	Universiti Teknologi Malaysia
building	UTM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Teknologi Malaysia
content_source	UTM Institutional Repository
url_provider	http://eprints.utm.my/
topic	TP Chemical technology
spellingShingle	TP Chemical technology Kek, Siok Peng Chin, Nyuk Ling Tan, Sheau Wei Yusof, Yus Aniza Chua, Lee Suan Comparison of DNA extraction methods for entomological origin identification of honey using simple additive weighting method
description	Four different DNA protocols were compared to obtain the best method for DNA extraction of bees in honey samples. The efficiency was evaluated in terms of DNA concentration and purity, PCR amplification capacity targeting mitochondrial 16S rRNA gene, and execution time. The most efficient DNA extraction method was selected using simple additive weighting (SAW) analysis. The DNeasy method with silica membrane spin-column format scored highest in the final ranking of SAW compared to CTAB-based, Wizard and NucleoSpin methods. It yielded extracted DNA with sufficient concentration (second highest), acceptable purity (1.5–2.0), amplifiable for 16S rRNA gene region, and required least extraction time. This recommended DNA extraction protocol suggests that genomic methodologies using a 150 bp amplicon of the 16S rRNA gene of bees enables identification of the entomological origin of honey.
format	Article
author	Kek, Siok Peng Chin, Nyuk Ling Tan, Sheau Wei Yusof, Yus Aniza Chua, Lee Suan
author_facet	Kek, Siok Peng Chin, Nyuk Ling Tan, Sheau Wei Yusof, Yus Aniza Chua, Lee Suan
author_sort	Kek, Siok Peng
title	Comparison of DNA extraction methods for entomological origin identification of honey using simple additive weighting method
title_short	Comparison of DNA extraction methods for entomological origin identification of honey using simple additive weighting method
title_full	Comparison of DNA extraction methods for entomological origin identification of honey using simple additive weighting method
title_fullStr	Comparison of DNA extraction methods for entomological origin identification of honey using simple additive weighting method
title_full_unstemmed	Comparison of DNA extraction methods for entomological origin identification of honey using simple additive weighting method
title_sort	comparison of dna extraction methods for entomological origin identification of honey using simple additive weighting method
publisher	Blackwell Publishing Ltd
publishDate	2018
url	http://eprints.utm.my/id/eprint/86688/ http://dx.doi.org/10.1111/ijfs.13840
_version_	1680321081775226880
score	13.211869

Comparison of DNA extraction methods for entomological origin identification of honey using simple additive weighting method

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