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Comparison of biophysical properties characterized for microtissues cultured using microencapsulation and liquid crystal based 3D cell culture techniques

Growing three dimensional (3D) cells is an emerging research in tissue engineering. Biophysical properties of the 3D cells regulate the cells growth, drug diffusion dynamics and gene expressions. Scaffold based or scaffoldless techniques for 3D cell cultures are rarely being compared in terms of the...

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Main Authors:	Soon, Chin Fhong, Tee, Kian Sek, Wong, Soon Chuan, Nayan, Nafarizal, Sundra, Sargunan, Ahmad, Mohd. Khairul, Sefat, Farshid, Sultana, Naznin, Youseffi, Mansour
Format:	Article
Published:	Springer Netherlands 2018
Subjects:	Q Science (General)
Online Access:	http://eprints.utm.my/id/eprint/86106/ http://dx.doi.org/10.1007/s10616-017-0168-2
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spelling	my.utm.861062020-08-30T08:56:12Z http://eprints.utm.my/id/eprint/86106/ Comparison of biophysical properties characterized for microtissues cultured using microencapsulation and liquid crystal based 3D cell culture techniques Soon, Chin Fhong Tee, Kian Sek Wong, Soon Chuan Nayan, Nafarizal Sundra, Sargunan Ahmad, Mohd. Khairul Sefat, Farshid Sultana, Naznin Youseffi, Mansour Q Science (General) Growing three dimensional (3D) cells is an emerging research in tissue engineering. Biophysical properties of the 3D cells regulate the cells growth, drug diffusion dynamics and gene expressions. Scaffold based or scaffoldless techniques for 3D cell cultures are rarely being compared in terms of the physical features of the microtissues produced. The biophysical properties of the microtissues cultured using scaffold based microencapsulation by flicking and scaffoldless liquid crystal (LC) based techniques were characterized. Flicking technique produced high yield and highly reproducible microtissues of keratinocyte cell lines in alginate microcapsules at approximately 350 ± 12 pieces per culture. However, microtissues grown on the LC substrates yielded at lower quantity of 58 ± 21 pieces per culture. The sizes of the microtissues produced using alginate microcapsules and LC substrates were 250 ± 25 μm and 141 ± 70 μm, respectively. In both techniques, cells remodeled into microtissues via different growth phases and showed good integrity of cells in field-emission scanning microscopy (FE-SEM). Microencapsulation packed the cells in alginate scaffolds of polysaccharides with limited spaces for motility. Whereas, LC substrates allowed the cells to migrate and self-stacking into multilayered structures as revealed by the nuclei stainings. The cells cultured using both techniques were found viable based on the live and dead cell stainings. Stained histological sections showed that both techniques produced cell models that closely replicate the intrinsic physiological conditions. Alginate microcapsulation and LC based techniques produced microtissues containing similar bio-macromolecules but they did not alter the main absorption bands of microtissues as revealed by the Fourier transform infrared spectroscopy. Cell growth, structural organization, morphology and surface structures for 3D microtissues cultured using both techniques appeared to be different and might be suitable for different applications. Springer Netherlands 2018-02 Article PeerReviewed Soon, Chin Fhong and Tee, Kian Sek and Wong, Soon Chuan and Nayan, Nafarizal and Sundra, Sargunan and Ahmad, Mohd. Khairul and Sefat, Farshid and Sultana, Naznin and Youseffi, Mansour (2018) Comparison of biophysical properties characterized for microtissues cultured using microencapsulation and liquid crystal based 3D cell culture techniques. Cytotechnology, 70 (1). pp. 13-29. ISSN 0920-9069 http://dx.doi.org/10.1007/s10616-017-0168-2
institution	Universiti Teknologi Malaysia
building	UTM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Teknologi Malaysia
content_source	UTM Institutional Repository
url_provider	http://eprints.utm.my/
topic	Q Science (General)
spellingShingle	Q Science (General) Soon, Chin Fhong Tee, Kian Sek Wong, Soon Chuan Nayan, Nafarizal Sundra, Sargunan Ahmad, Mohd. Khairul Sefat, Farshid Sultana, Naznin Youseffi, Mansour Comparison of biophysical properties characterized for microtissues cultured using microencapsulation and liquid crystal based 3D cell culture techniques
description	Growing three dimensional (3D) cells is an emerging research in tissue engineering. Biophysical properties of the 3D cells regulate the cells growth, drug diffusion dynamics and gene expressions. Scaffold based or scaffoldless techniques for 3D cell cultures are rarely being compared in terms of the physical features of the microtissues produced. The biophysical properties of the microtissues cultured using scaffold based microencapsulation by flicking and scaffoldless liquid crystal (LC) based techniques were characterized. Flicking technique produced high yield and highly reproducible microtissues of keratinocyte cell lines in alginate microcapsules at approximately 350 ± 12 pieces per culture. However, microtissues grown on the LC substrates yielded at lower quantity of 58 ± 21 pieces per culture. The sizes of the microtissues produced using alginate microcapsules and LC substrates were 250 ± 25 μm and 141 ± 70 μm, respectively. In both techniques, cells remodeled into microtissues via different growth phases and showed good integrity of cells in field-emission scanning microscopy (FE-SEM). Microencapsulation packed the cells in alginate scaffolds of polysaccharides with limited spaces for motility. Whereas, LC substrates allowed the cells to migrate and self-stacking into multilayered structures as revealed by the nuclei stainings. The cells cultured using both techniques were found viable based on the live and dead cell stainings. Stained histological sections showed that both techniques produced cell models that closely replicate the intrinsic physiological conditions. Alginate microcapsulation and LC based techniques produced microtissues containing similar bio-macromolecules but they did not alter the main absorption bands of microtissues as revealed by the Fourier transform infrared spectroscopy. Cell growth, structural organization, morphology and surface structures for 3D microtissues cultured using both techniques appeared to be different and might be suitable for different applications.
format	Article
author	Soon, Chin Fhong Tee, Kian Sek Wong, Soon Chuan Nayan, Nafarizal Sundra, Sargunan Ahmad, Mohd. Khairul Sefat, Farshid Sultana, Naznin Youseffi, Mansour
author_facet	Soon, Chin Fhong Tee, Kian Sek Wong, Soon Chuan Nayan, Nafarizal Sundra, Sargunan Ahmad, Mohd. Khairul Sefat, Farshid Sultana, Naznin Youseffi, Mansour
author_sort	Soon, Chin Fhong
title	Comparison of biophysical properties characterized for microtissues cultured using microencapsulation and liquid crystal based 3D cell culture techniques
title_short	Comparison of biophysical properties characterized for microtissues cultured using microencapsulation and liquid crystal based 3D cell culture techniques
title_full	Comparison of biophysical properties characterized for microtissues cultured using microencapsulation and liquid crystal based 3D cell culture techniques
title_fullStr	Comparison of biophysical properties characterized for microtissues cultured using microencapsulation and liquid crystal based 3D cell culture techniques
title_full_unstemmed	Comparison of biophysical properties characterized for microtissues cultured using microencapsulation and liquid crystal based 3D cell culture techniques
title_sort	comparison of biophysical properties characterized for microtissues cultured using microencapsulation and liquid crystal based 3d cell culture techniques
publisher	Springer Netherlands
publishDate	2018
url	http://eprints.utm.my/id/eprint/86106/ http://dx.doi.org/10.1007/s10616-017-0168-2
_version_	1677781131383013376
score	13.211869

Comparison of biophysical properties characterized for microtissues cultured using microencapsulation and liquid crystal based 3D cell culture techniques

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