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Sniffer bees as a reliable tool for Andrographis paniculata detection

Honey bees of Apis mellifera could be trained to be highly reliable sniffers for the detection of Andrographis paniculata using the classical Pavlovian conditioning training method with high success rate, > 80% based on the proboscis extension reflex as a positive response to the presence of the...

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Main Authors: Kerk, Wen Chiann, Chua, Lee Suan, Sarmidi, Mohamad Roji, Abd. Aziz, Ramlan Abdul
Format: Article
Published: Springer Nature 2018
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TP Chemical technology
Online Access:http://eprints.utm.my/id/eprint/85923/
http://dx.doi.org/10.1007/s11220-018-0194-y
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spelling my.utm.859232020-07-30T07:39:00Z http://eprints.utm.my/id/eprint/85923/ Sniffer bees as a reliable tool for Andrographis paniculata detection Kerk, Wen Chiann Chua, Lee Suan Sarmidi, Mohamad Roji Abd. Aziz, Ramlan Abdul TP Chemical technology Honey bees of Apis mellifera could be trained to be highly reliable sniffers for the detection of Andrographis paniculata using the classical Pavlovian conditioning training method with high success rate, > 80% based on the proboscis extension reflex as a positive response to the presence of the herb. The success rate of sniffer bees was found to be in a temperature dependent manner, but not significantly affected by the heating duration (5–110 min). The variance of 7.7% success rate was observed for the heating temperature ranged 50–120 °C with the highest success rate (92.7%) at 100 °C. This could be due to the content of signature compounds released from the heated herbal samples. Three signature compounds such as dihydroactinidiolide, apiol and 6,10,14-trimethyl-2-pentadecanone were proposed to be the volatile marker of the herb since their concentrations changed in accordance with the temperature profile and success rate of sniffer bees. The volatile compounds were extracted by divinylbenzene and carboxen coated polydimethylsiloxane fiber in the headspace of solid phase micro-extraction before analyzed by GC–MS for identification. Almost 50% success rate could be achieved using the minimum amount of 20 mg herbal samples. High selectivity of the sniffer bees has also been proven by no response to another morphologically similar herb, Clinacanthus nutans which was also heat-treated in the similar manner. The sniffer bees also showed to exhibit 80% success rate to detect A. paniculata mixed with 50% C. nutans as interference in a mixture. Springer Nature 2018 Article PeerReviewed Kerk, Wen Chiann and Chua, Lee Suan and Sarmidi, Mohamad Roji and Abd. Aziz, Ramlan Abdul (2018) Sniffer bees as a reliable tool for Andrographis paniculata detection. Sensing and Imaging, 19 (1). p. 9. ISSN 1557-2064 http://dx.doi.org/10.1007/s11220-018-0194-y
institution Universiti Teknologi Malaysia
building UTM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Malaysia
content_source UTM Institutional Repository
url_provider http://eprints.utm.my/
topic TP Chemical technology
spellingShingle TP Chemical technology
Kerk, Wen Chiann
Chua, Lee Suan
Sarmidi, Mohamad Roji
Abd. Aziz, Ramlan Abdul
Sniffer bees as a reliable tool for Andrographis paniculata detection
description Honey bees of Apis mellifera could be trained to be highly reliable sniffers for the detection of Andrographis paniculata using the classical Pavlovian conditioning training method with high success rate, > 80% based on the proboscis extension reflex as a positive response to the presence of the herb. The success rate of sniffer bees was found to be in a temperature dependent manner, but not significantly affected by the heating duration (5–110 min). The variance of 7.7% success rate was observed for the heating temperature ranged 50–120 °C with the highest success rate (92.7%) at 100 °C. This could be due to the content of signature compounds released from the heated herbal samples. Three signature compounds such as dihydroactinidiolide, apiol and 6,10,14-trimethyl-2-pentadecanone were proposed to be the volatile marker of the herb since their concentrations changed in accordance with the temperature profile and success rate of sniffer bees. The volatile compounds were extracted by divinylbenzene and carboxen coated polydimethylsiloxane fiber in the headspace of solid phase micro-extraction before analyzed by GC–MS for identification. Almost 50% success rate could be achieved using the minimum amount of 20 mg herbal samples. High selectivity of the sniffer bees has also been proven by no response to another morphologically similar herb, Clinacanthus nutans which was also heat-treated in the similar manner. The sniffer bees also showed to exhibit 80% success rate to detect A. paniculata mixed with 50% C. nutans as interference in a mixture.
format Article
author Kerk, Wen Chiann
Chua, Lee Suan
Sarmidi, Mohamad Roji
Abd. Aziz, Ramlan Abdul
author_facet Kerk, Wen Chiann
Chua, Lee Suan
Sarmidi, Mohamad Roji
Abd. Aziz, Ramlan Abdul
author_sort Kerk, Wen Chiann
title Sniffer bees as a reliable tool for Andrographis paniculata detection
title_short Sniffer bees as a reliable tool for Andrographis paniculata detection
title_full Sniffer bees as a reliable tool for Andrographis paniculata detection
title_fullStr Sniffer bees as a reliable tool for Andrographis paniculata detection
title_full_unstemmed Sniffer bees as a reliable tool for Andrographis paniculata detection
title_sort sniffer bees as a reliable tool for andrographis paniculata detection
publisher Springer Nature
publishDate 2018
url http://eprints.utm.my/id/eprint/85923/
http://dx.doi.org/10.1007/s11220-018-0194-y
_version_ 1674066228226818048
score 13.211869

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