Cover Image

A fast, sensitive and validated technique for eurycomanone and cordycepin quantitation using UPLCaEuro'MS/MS

Herbs and herbal based products have been widely used, but some products might contain no herbal ingredient as claimed in the product label. Therefore, it is crucial to develop a fast, sensitive and reliable method to analyze the herbs and their finished products. The roots of Eurycoma longifolia, a...

Full description

Saved in:
Bibliographic Details
Main Authors: Chua, Lee Suan, Abdul Latiff, Norliza, Ware, Ismail
Format: Article
Published: Pleiades Publishing 2018
Subjects:
TP Chemical technology
Online Access:http://eprints.utm.my/id/eprint/85624/
http://dx.doi.org/10.1134/S1061934818020065
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.utm.85624
record_format eprints
spelling my.utm.856242020-07-07T04:59:57Z http://eprints.utm.my/id/eprint/85624/ A fast, sensitive and validated technique for eurycomanone and cordycepin quantitation using UPLCaEuro'MS/MS Chua, Lee Suan Abdul Latiff, Norliza Ware, Ismail TP Chemical technology Herbs and herbal based products have been widely used, but some products might contain no herbal ingredient as claimed in the product label. Therefore, it is crucial to develop a fast, sensitive and reliable method to analyze the herbs and their finished products. The roots of Eurycoma longifolia, and the health supplement of E. longifolia and Cordyceps were used as the sample matrices in this study. Sonication assisted extraction was applied to extract the marker compounds, namely eurycomanone and cordycepin from E. longifolia and Cordyceps, respectively. The presence of the marker compounds was established by high throughput ultra-performance liquid chromatography tandem mass spectrometry (UPLC‒MS/MS) using a highly sensitive and selective technique of multiple reaction monitoring (MRM). The positive ion transitions in MRM for eurycomanone and cordycepin are m/z 409 → 391 and m/z 252 → 136, respectively. The test method was validated for its robustness, accuracy, precision, linearity, detection and quantitation limits, as well as estimated for its measurement uncertainty. Pleiades Publishing 2018-02 Article PeerReviewed Chua, Lee Suan and Abdul Latiff, Norliza and Ware, Ismail (2018) A fast, sensitive and validated technique for eurycomanone and cordycepin quantitation using UPLCaEuro'MS/MS. Journal of Analytical Chemistry, 73 (2). pp. 167-175. ISSN 1061-9348 http://dx.doi.org/10.1134/S1061934818020065
institution Universiti Teknologi Malaysia
building UTM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Malaysia
content_source UTM Institutional Repository
url_provider http://eprints.utm.my/
topic TP Chemical technology
spellingShingle TP Chemical technology
Chua, Lee Suan
Abdul Latiff, Norliza
Ware, Ismail
A fast, sensitive and validated technique for eurycomanone and cordycepin quantitation using UPLCaEuro'MS/MS
description Herbs and herbal based products have been widely used, but some products might contain no herbal ingredient as claimed in the product label. Therefore, it is crucial to develop a fast, sensitive and reliable method to analyze the herbs and their finished products. The roots of Eurycoma longifolia, and the health supplement of E. longifolia and Cordyceps were used as the sample matrices in this study. Sonication assisted extraction was applied to extract the marker compounds, namely eurycomanone and cordycepin from E. longifolia and Cordyceps, respectively. The presence of the marker compounds was established by high throughput ultra-performance liquid chromatography tandem mass spectrometry (UPLC‒MS/MS) using a highly sensitive and selective technique of multiple reaction monitoring (MRM). The positive ion transitions in MRM for eurycomanone and cordycepin are m/z 409 → 391 and m/z 252 → 136, respectively. The test method was validated for its robustness, accuracy, precision, linearity, detection and quantitation limits, as well as estimated for its measurement uncertainty.
format Article
author Chua, Lee Suan
Abdul Latiff, Norliza
Ware, Ismail
author_facet Chua, Lee Suan
Abdul Latiff, Norliza
Ware, Ismail
author_sort Chua, Lee Suan
title A fast, sensitive and validated technique for eurycomanone and cordycepin quantitation using UPLCaEuro'MS/MS
title_short A fast, sensitive and validated technique for eurycomanone and cordycepin quantitation using UPLCaEuro'MS/MS
title_full A fast, sensitive and validated technique for eurycomanone and cordycepin quantitation using UPLCaEuro'MS/MS
title_fullStr A fast, sensitive and validated technique for eurycomanone and cordycepin quantitation using UPLCaEuro'MS/MS
title_full_unstemmed A fast, sensitive and validated technique for eurycomanone and cordycepin quantitation using UPLCaEuro'MS/MS
title_sort fast, sensitive and validated technique for eurycomanone and cordycepin quantitation using uplcaeuro'ms/ms
publisher Pleiades Publishing
publishDate 2018
url http://eprints.utm.my/id/eprint/85624/
http://dx.doi.org/10.1134/S1061934818020065
_version_ 1672610560669122560
score 13.211869

Similar Items