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Significance of bio-treatment by acid washing for enlargement of arsenic desorption in indigenous arsenic-resistant bacteria from gold mine

Mining activities can lead to the generation of large quantities of heavy metal, specifically arsenic which is released from a gold mine, causing widespread contamination of the ecosystem. Removal of carcinogenic and toxic arsenic from wastewater is essential for the safety of water that may be used...

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Main Authors: Altowayti, Wahid Ali Hamood, Dahawi, Abdullah Aysh, Shahir, Shafinaz
Format: Article
Language:English
Published: Penerbit UTM Press 2020
Subjects:
QD Chemistry
Online Access:http://eprints.utm.my/id/eprint/85595/1/ShafinazShahir2020_SignificanceofBioTreatmentbyAcidWashing.pdf
http://eprints.utm.my/id/eprint/85595/
https://mjfas.utm.my/index.php/mjfas/article/view/1422
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spelling my.utm.855952020-06-30T08:54:07Z http://eprints.utm.my/id/eprint/85595/ Significance of bio-treatment by acid washing for enlargement of arsenic desorption in indigenous arsenic-resistant bacteria from gold mine Altowayti, Wahid Ali Hamood Dahawi, Abdullah Aysh Shahir, Shafinaz QD Chemistry Mining activities can lead to the generation of large quantities of heavy metal, specifically arsenic which is released from a gold mine, causing widespread contamination of the ecosystem. Removal of carcinogenic and toxic arsenic from wastewater is essential for the safety of water that may be used for irrigation or drinking. In this study, three different of indigenous arsenic resistant bacterial strains were isolated from gold mine environment, Bacillus thuringiensis strain WS3, Pseudomonas stutzeri strain WS9, and Micrococcus yunnanensis strain WS11. WS9, WS3, and WS11 reached stationary phase after eight, ten, and seven hours, respectively, at 37 °C when grown in LB with arsenic. Gram staining showed WS9 as gram-negative rods, WS3 as grampositive rods, and WS11 as gram-positive cocci. From the Silver nitrate test, WS3 and WS11 reduced As (V) to As (III) while WS9 oxidized As (III) to As (V). The desorption of arsenic using acid washing and parameters affecting the desorption of arsenic such as acid concentration, time, adsorbent dosage, and different volume of acid solution were investigated. The batch experiments were carried out using bacterial biomass cultured in LB with 2 mM arsenite (III) and 5 mM arsenate (V). Optimum conditions for desorption arsenic were determined, being 1 M acid concentration at 37 °C and 2 hours of contact with (50 mg) bacterial biomass in 100 ml acid solution. The removal of arsenite and arsenate increased after acid washing of bacterial biomass of the three strains. Consequently, desorption of arsenic using acid washing is essential for biomass regeneration. Penerbit UTM Press 2020 Article PeerReviewed application/pdf en http://eprints.utm.my/id/eprint/85595/1/ShafinazShahir2020_SignificanceofBioTreatmentbyAcidWashing.pdf Altowayti, Wahid Ali Hamood and Dahawi, Abdullah Aysh and Shahir, Shafinaz (2020) Significance of bio-treatment by acid washing for enlargement of arsenic desorption in indigenous arsenic-resistant bacteria from gold mine. Malaysian Journal of Fundamental and Applied Sciences, 16 (2). pp. 190-195. ISSN 2289-5981 https://mjfas.utm.my/index.php/mjfas/article/view/1422
institution Universiti Teknologi Malaysia
building UTM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Malaysia
content_source UTM Institutional Repository
url_provider http://eprints.utm.my/
language English
topic QD Chemistry
spellingShingle QD Chemistry
Altowayti, Wahid Ali Hamood
Dahawi, Abdullah Aysh
Shahir, Shafinaz
Significance of bio-treatment by acid washing for enlargement of arsenic desorption in indigenous arsenic-resistant bacteria from gold mine
description Mining activities can lead to the generation of large quantities of heavy metal, specifically arsenic which is released from a gold mine, causing widespread contamination of the ecosystem. Removal of carcinogenic and toxic arsenic from wastewater is essential for the safety of water that may be used for irrigation or drinking. In this study, three different of indigenous arsenic resistant bacterial strains were isolated from gold mine environment, Bacillus thuringiensis strain WS3, Pseudomonas stutzeri strain WS9, and Micrococcus yunnanensis strain WS11. WS9, WS3, and WS11 reached stationary phase after eight, ten, and seven hours, respectively, at 37 °C when grown in LB with arsenic. Gram staining showed WS9 as gram-negative rods, WS3 as grampositive rods, and WS11 as gram-positive cocci. From the Silver nitrate test, WS3 and WS11 reduced As (V) to As (III) while WS9 oxidized As (III) to As (V). The desorption of arsenic using acid washing and parameters affecting the desorption of arsenic such as acid concentration, time, adsorbent dosage, and different volume of acid solution were investigated. The batch experiments were carried out using bacterial biomass cultured in LB with 2 mM arsenite (III) and 5 mM arsenate (V). Optimum conditions for desorption arsenic were determined, being 1 M acid concentration at 37 °C and 2 hours of contact with (50 mg) bacterial biomass in 100 ml acid solution. The removal of arsenite and arsenate increased after acid washing of bacterial biomass of the three strains. Consequently, desorption of arsenic using acid washing is essential for biomass regeneration.
format Article
author Altowayti, Wahid Ali Hamood
Dahawi, Abdullah Aysh
Shahir, Shafinaz
author_facet Altowayti, Wahid Ali Hamood
Dahawi, Abdullah Aysh
Shahir, Shafinaz
author_sort Altowayti, Wahid Ali Hamood
title Significance of bio-treatment by acid washing for enlargement of arsenic desorption in indigenous arsenic-resistant bacteria from gold mine
title_short Significance of bio-treatment by acid washing for enlargement of arsenic desorption in indigenous arsenic-resistant bacteria from gold mine
title_full Significance of bio-treatment by acid washing for enlargement of arsenic desorption in indigenous arsenic-resistant bacteria from gold mine
title_fullStr Significance of bio-treatment by acid washing for enlargement of arsenic desorption in indigenous arsenic-resistant bacteria from gold mine
title_full_unstemmed Significance of bio-treatment by acid washing for enlargement of arsenic desorption in indigenous arsenic-resistant bacteria from gold mine
title_sort significance of bio-treatment by acid washing for enlargement of arsenic desorption in indigenous arsenic-resistant bacteria from gold mine
publisher Penerbit UTM Press
publishDate 2020
url http://eprints.utm.my/id/eprint/85595/1/ShafinazShahir2020_SignificanceofBioTreatmentbyAcidWashing.pdf
http://eprints.utm.my/id/eprint/85595/
https://mjfas.utm.my/index.php/mjfas/article/view/1422
_version_ 1672610556724379648
score 13.211869

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