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Effect of cross-linked enzyme aggregates in hierarchically mesocellular mesoporous magnetic silica preparation conditions towards enzyme activity retention

This work aimed to optimize ten preparation factors that might influence the cellulase and xylanase activity retention of cross-linked enzyme aggregates in hierarchically mesocellular mesoporous magnetic silica. The factors were optimized using the fractional factorial design (210-5). The optimized...

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Main Authors: Sulaiman, Nurul Jannah, Abdul Rahman, Roshanida, Md. Illias, Rosli
Format: Article
Language:English
Published: Penerbit UTM Press 2019
Subjects:
TP Chemical technology
Online Access:http://eprints.utm.my/id/eprint/84966/1/RoshanidaAbdulRahman2019_EffectofCross-LinkedEnzymeAggregates.pdf
http://eprints.utm.my/id/eprint/84966/
https://dx.doi.org/10.11113/mjfas.v15n2019.1205
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spelling my.utm.849662020-02-29T13:49:47Z http://eprints.utm.my/id/eprint/84966/ Effect of cross-linked enzyme aggregates in hierarchically mesocellular mesoporous magnetic silica preparation conditions towards enzyme activity retention Sulaiman, Nurul Jannah Abdul Rahman, Roshanida Md. Illias, Rosli TP Chemical technology This work aimed to optimize ten preparation factors that might influence the cellulase and xylanase activity retention of cross-linked enzyme aggregates in hierarchically mesocellular mesoporous magnetic silica. The factors were optimized using the fractional factorial design (210-5). The optimized output was occurred at 2 mL of enzyme amount, magnet-to-enzyme ratio of 1:0.15, enzyme adsorption at 26°C and 162 rpm in 40 min, enzyme-to-precipitant ratio of 1:11, 0.05% (v/v) of glutaraldehyde concentration, and cross-linking process at 37°C and 300 rpm in 2 h. The factors were examined to observe the effect of every factor towards cellulase and xylanase acrivity retention. Penerbit UTM Press 2019 Article PeerReviewed application/pdf en http://eprints.utm.my/id/eprint/84966/1/RoshanidaAbdulRahman2019_EffectofCross-LinkedEnzymeAggregates.pdf Sulaiman, Nurul Jannah and Abdul Rahman, Roshanida and Md. Illias, Rosli (2019) Effect of cross-linked enzyme aggregates in hierarchically mesocellular mesoporous magnetic silica preparation conditions towards enzyme activity retention. Malaysian Journal of Fundamental and Applied Sciences, 15 (1). pp. 6-12. ISSN 2289-5981 https://dx.doi.org/10.11113/mjfas.v15n2019.1205 DOI:10.11113/mjfas.v15n2019.1205
institution Universiti Teknologi Malaysia
building UTM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Malaysia
content_source UTM Institutional Repository
url_provider http://eprints.utm.my/
language English
topic TP Chemical technology
spellingShingle TP Chemical technology
Sulaiman, Nurul Jannah
Abdul Rahman, Roshanida
Md. Illias, Rosli
Effect of cross-linked enzyme aggregates in hierarchically mesocellular mesoporous magnetic silica preparation conditions towards enzyme activity retention
description This work aimed to optimize ten preparation factors that might influence the cellulase and xylanase activity retention of cross-linked enzyme aggregates in hierarchically mesocellular mesoporous magnetic silica. The factors were optimized using the fractional factorial design (210-5). The optimized output was occurred at 2 mL of enzyme amount, magnet-to-enzyme ratio of 1:0.15, enzyme adsorption at 26°C and 162 rpm in 40 min, enzyme-to-precipitant ratio of 1:11, 0.05% (v/v) of glutaraldehyde concentration, and cross-linking process at 37°C and 300 rpm in 2 h. The factors were examined to observe the effect of every factor towards cellulase and xylanase acrivity retention.
format Article
author Sulaiman, Nurul Jannah
Abdul Rahman, Roshanida
Md. Illias, Rosli
author_facet Sulaiman, Nurul Jannah
Abdul Rahman, Roshanida
Md. Illias, Rosli
author_sort Sulaiman, Nurul Jannah
title Effect of cross-linked enzyme aggregates in hierarchically mesocellular mesoporous magnetic silica preparation conditions towards enzyme activity retention
title_short Effect of cross-linked enzyme aggregates in hierarchically mesocellular mesoporous magnetic silica preparation conditions towards enzyme activity retention
title_full Effect of cross-linked enzyme aggregates in hierarchically mesocellular mesoporous magnetic silica preparation conditions towards enzyme activity retention
title_fullStr Effect of cross-linked enzyme aggregates in hierarchically mesocellular mesoporous magnetic silica preparation conditions towards enzyme activity retention
title_full_unstemmed Effect of cross-linked enzyme aggregates in hierarchically mesocellular mesoporous magnetic silica preparation conditions towards enzyme activity retention
title_sort effect of cross-linked enzyme aggregates in hierarchically mesocellular mesoporous magnetic silica preparation conditions towards enzyme activity retention
publisher Penerbit UTM Press
publishDate 2019
url http://eprints.utm.my/id/eprint/84966/1/RoshanidaAbdulRahman2019_EffectofCross-LinkedEnzymeAggregates.pdf
http://eprints.utm.my/id/eprint/84966/
https://dx.doi.org/10.11113/mjfas.v15n2019.1205
_version_ 1662754332326494208
score 13.211869

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