Staff View: Low concentration of Bisphenol a induces proliferation of gastric cancer cells, HGC-27

Low concentration of Bisphenol a induces proliferation of gastric cancer cells, HGC-27

Bisphenol A, an endocrine disrupting compound that affects human homeostasis. Studies on BPA are focusing on the impact of BPA in reproductive function and brain development. However, the effect of BPA on gut especially gastric cells is not well explored. Gut is directly in contact with ingested BPA...

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Main Authors:	Hanafi, Noorul Izzati, Sheikh Abdul Kadir, Siti Hamimah, Musa, Maslinda, Othman, Mohd. Hafiz Dzarfan, Kamaludin, Roziana, Zulkifli, Nur Amira, Abd. Latip, Normala, A. Karim, Zeti Rahayu
Format:	Article
Language:	English
Published:	Penerbit UTM Press 2019
Subjects:	TP Chemical technology
Online Access:	http://eprints.utm.my/id/eprint/84783/1/RozianaKamaludin2019_LowConcentrationofBisphenolaInduces.pdf http://eprints.utm.my/id/eprint/84783/ https://dx.doi.org/10.11113/jt.v81.13670
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spelling	my.utm.847832020-02-27T04:58:48Z http://eprints.utm.my/id/eprint/84783/ Low concentration of Bisphenol a induces proliferation of gastric cancer cells, HGC-27 Hanafi, Noorul Izzati Sheikh Abdul Kadir, Siti Hamimah Musa, Maslinda Othman, Mohd. Hafiz Dzarfan Kamaludin, Roziana Zulkifli, Nur Amira Abd. Latip, Normala A. Karim, Zeti Rahayu TP Chemical technology Bisphenol A, an endocrine disrupting compound that affects human homeostasis. Studies on BPA are focusing on the impact of BPA in reproductive function and brain development. However, the effect of BPA on gut especially gastric cells is not well explored. Gut is directly in contact with ingested BPA; therefore, we aimed to determine the effect of BPA exposure on gastric cells proliferation at safe recommended concentration. Human gastric cancer cells (HGC-27) were treated with BPA at different concentration (low: 10-9M, 10-7M; high10-5M, 10-4M) and time point (24 hr, 48 hr, 72 hr). Cell viability assays were determined using MTS assay. Cells were further stained with Alexa Fluor-635 (F-actin) and Fluorescein (Hif-1α) protein for immunocytofluorescence. Data were analysed using ANOVA (p<0.05, n≥3). Cells treated with 10-9M BPA showed significance increase of cell viability after 48 hr (Mean ±SEM; 146%±0.03, p=0.01) and 72 hr (113%±0.03, p=0.00) compared to 24 hr treatment (77%±0.11, p=0.002). Similarly, cell treated with 10-7M BPA showed a significance increase after 48 hr (141%±0.03, p=0.03) and 72 hr (190%±0.03, p=0.02) compared to 24 hr cells treated with 10-7M (88%±0.05, p=0.01) and untreated (100%±0.07). Lower concentration of BPA increases the condensation of F-actin in all HGC-27 cells. Meanwhile, translocation of Hif-1α protein were observed in all BPA-exposed cells. Findings of this study revealed that BPA induced proliferation and condensation of F-actin structure of gastric cancer cells at low concentration. Penerbit UTM Press 2019 Article PeerReviewed application/pdf en http://eprints.utm.my/id/eprint/84783/1/RozianaKamaludin2019_LowConcentrationofBisphenolaInduces.pdf Hanafi, Noorul Izzati and Sheikh Abdul Kadir, Siti Hamimah and Musa, Maslinda and Othman, Mohd. Hafiz Dzarfan and Kamaludin, Roziana and Zulkifli, Nur Amira and Abd. Latip, Normala and A. Karim, Zeti Rahayu (2019) Low concentration of Bisphenol a induces proliferation of gastric cancer cells, HGC-27. Jurnal Teknologi, 81 (6). pp. 115-121. ISSN 2180-3722 https://dx.doi.org/10.11113/jt.v81.13670 DOI:10.11113/jt.v81.13670
institution	Universiti Teknologi Malaysia
building	UTM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Teknologi Malaysia
content_source	UTM Institutional Repository
url_provider	http://eprints.utm.my/
language	English
topic	TP Chemical technology
spellingShingle	TP Chemical technology Hanafi, Noorul Izzati Sheikh Abdul Kadir, Siti Hamimah Musa, Maslinda Othman, Mohd. Hafiz Dzarfan Kamaludin, Roziana Zulkifli, Nur Amira Abd. Latip, Normala A. Karim, Zeti Rahayu Low concentration of Bisphenol a induces proliferation of gastric cancer cells, HGC-27
description	Bisphenol A, an endocrine disrupting compound that affects human homeostasis. Studies on BPA are focusing on the impact of BPA in reproductive function and brain development. However, the effect of BPA on gut especially gastric cells is not well explored. Gut is directly in contact with ingested BPA; therefore, we aimed to determine the effect of BPA exposure on gastric cells proliferation at safe recommended concentration. Human gastric cancer cells (HGC-27) were treated with BPA at different concentration (low: 10-9M, 10-7M; high10-5M, 10-4M) and time point (24 hr, 48 hr, 72 hr). Cell viability assays were determined using MTS assay. Cells were further stained with Alexa Fluor-635 (F-actin) and Fluorescein (Hif-1α) protein for immunocytofluorescence. Data were analysed using ANOVA (p<0.05, n≥3). Cells treated with 10-9M BPA showed significance increase of cell viability after 48 hr (Mean ±SEM; 146%±0.03, p=0.01) and 72 hr (113%±0.03, p=0.00) compared to 24 hr treatment (77%±0.11, p=0.002). Similarly, cell treated with 10-7M BPA showed a significance increase after 48 hr (141%±0.03, p=0.03) and 72 hr (190%±0.03, p=0.02) compared to 24 hr cells treated with 10-7M (88%±0.05, p=0.01) and untreated (100%±0.07). Lower concentration of BPA increases the condensation of F-actin in all HGC-27 cells. Meanwhile, translocation of Hif-1α protein were observed in all BPA-exposed cells. Findings of this study revealed that BPA induced proliferation and condensation of F-actin structure of gastric cancer cells at low concentration.
format	Article
author	Hanafi, Noorul Izzati Sheikh Abdul Kadir, Siti Hamimah Musa, Maslinda Othman, Mohd. Hafiz Dzarfan Kamaludin, Roziana Zulkifli, Nur Amira Abd. Latip, Normala A. Karim, Zeti Rahayu
author_facet	Hanafi, Noorul Izzati Sheikh Abdul Kadir, Siti Hamimah Musa, Maslinda Othman, Mohd. Hafiz Dzarfan Kamaludin, Roziana Zulkifli, Nur Amira Abd. Latip, Normala A. Karim, Zeti Rahayu
author_sort	Hanafi, Noorul Izzati
title	Low concentration of Bisphenol a induces proliferation of gastric cancer cells, HGC-27
title_short	Low concentration of Bisphenol a induces proliferation of gastric cancer cells, HGC-27
title_full	Low concentration of Bisphenol a induces proliferation of gastric cancer cells, HGC-27
title_fullStr	Low concentration of Bisphenol a induces proliferation of gastric cancer cells, HGC-27
title_full_unstemmed	Low concentration of Bisphenol a induces proliferation of gastric cancer cells, HGC-27
title_sort	low concentration of bisphenol a induces proliferation of gastric cancer cells, hgc-27
publisher	Penerbit UTM Press
publishDate	2019
url	http://eprints.utm.my/id/eprint/84783/1/RozianaKamaludin2019_LowConcentrationofBisphenolaInduces.pdf http://eprints.utm.my/id/eprint/84783/ https://dx.doi.org/10.11113/jt.v81.13670
_version_	1662754306805202944
score	13.211869

Low concentration of Bisphenol a induces proliferation of gastric cancer cells, HGC-27

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