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Extraction of bromelain from pineapple waste and purification by column chromatograhy

Bromelain has potential and demand in worldwide applications especially in pharmaceutical industries but commercial bromelain is very expensive due to high cost in extraction and purification method. Pineapple wastes are proportionally increasing with the production of pineapples concurrently. Pinea...

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Main Authors: Abd. Samad, Azman, Wan Dagang, Wan Rosmiza Zana, Jamaluddin, Haryati, Arsad, Noor Khaleeda
Format: Conference or Workshop Item
Published: 2018
Subjects:
Q Science (General)
Online Access:http://eprints.utm.my/id/eprint/83936/
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spelling my.utm.839362019-11-17T08:08:59Z http://eprints.utm.my/id/eprint/83936/ Extraction of bromelain from pineapple waste and purification by column chromatograhy Abd. Samad, Azman Wan Dagang, Wan Rosmiza Zana Jamaluddin, Haryati Arsad, Noor Khaleeda Q Science (General) Bromelain has potential and demand in worldwide applications especially in pharmaceutical industries but commercial bromelain is very expensive due to high cost in extraction and purification method. Pineapple wastes are proportionally increasing with the production of pineapples concurrently. Pineapple waste not used in any production yet and has no appropriate destinations. Waste disposal become growing problem as it usually prone to microbial spoilage and serious environmental problems. Therefore, this study is to extract bromelain in highest purified form from pineapple waste (crown and stem) from different species in different cultivars at reduced cost. Furthermore, it is economically and environmental friendly. Some studies have demonstrated that bromelain has potential in food, cosmetic and pharmaceutical industries. Bromelain activity was determined by casein digestion unit (CDU) and well diffusion method using skim milk and casein plate. Moris shows the extremely high of bromelain activity in crown (11.852 ±0.555) U/ml while Yankee shows the lowest bromelain activity in stem (0.580±0.091) U/ml. Crown has higher bromelain content compared to stem. Bromelain also was tested with different protein substrate such as fibrin to show the fibrinolytic activity. The purification method used is ion exchange chromatography by using carboxymethy-cellulose (CMC), gel filtration, SDSPAGE and HPLC. 2018-08 Conference or Workshop Item PeerReviewed Abd. Samad, Azman and Wan Dagang, Wan Rosmiza Zana and Jamaluddin, Haryati and Arsad, Noor Khaleeda (2018) Extraction of bromelain from pineapple waste and purification by column chromatograhy. In: The 7th International Graduate Conference on Engineering, Science & Humanities, 13 August 2018 through 15 August 2018, UTM Skudai.
institution Universiti Teknologi Malaysia
building UTM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Malaysia
content_source UTM Institutional Repository
url_provider http://eprints.utm.my/
topic Q Science (General)
spellingShingle Q Science (General)
Abd. Samad, Azman
Wan Dagang, Wan Rosmiza Zana
Jamaluddin, Haryati
Arsad, Noor Khaleeda
Extraction of bromelain from pineapple waste and purification by column chromatograhy
description Bromelain has potential and demand in worldwide applications especially in pharmaceutical industries but commercial bromelain is very expensive due to high cost in extraction and purification method. Pineapple wastes are proportionally increasing with the production of pineapples concurrently. Pineapple waste not used in any production yet and has no appropriate destinations. Waste disposal become growing problem as it usually prone to microbial spoilage and serious environmental problems. Therefore, this study is to extract bromelain in highest purified form from pineapple waste (crown and stem) from different species in different cultivars at reduced cost. Furthermore, it is economically and environmental friendly. Some studies have demonstrated that bromelain has potential in food, cosmetic and pharmaceutical industries. Bromelain activity was determined by casein digestion unit (CDU) and well diffusion method using skim milk and casein plate. Moris shows the extremely high of bromelain activity in crown (11.852 ±0.555) U/ml while Yankee shows the lowest bromelain activity in stem (0.580±0.091) U/ml. Crown has higher bromelain content compared to stem. Bromelain also was tested with different protein substrate such as fibrin to show the fibrinolytic activity. The purification method used is ion exchange chromatography by using carboxymethy-cellulose (CMC), gel filtration, SDSPAGE and HPLC.
format Conference or Workshop Item
author Abd. Samad, Azman
Wan Dagang, Wan Rosmiza Zana
Jamaluddin, Haryati
Arsad, Noor Khaleeda
author_facet Abd. Samad, Azman
Wan Dagang, Wan Rosmiza Zana
Jamaluddin, Haryati
Arsad, Noor Khaleeda
author_sort Abd. Samad, Azman
title Extraction of bromelain from pineapple waste and purification by column chromatograhy
title_short Extraction of bromelain from pineapple waste and purification by column chromatograhy
title_full Extraction of bromelain from pineapple waste and purification by column chromatograhy
title_fullStr Extraction of bromelain from pineapple waste and purification by column chromatograhy
title_full_unstemmed Extraction of bromelain from pineapple waste and purification by column chromatograhy
title_sort extraction of bromelain from pineapple waste and purification by column chromatograhy
publishDate 2018
url http://eprints.utm.my/id/eprint/83936/
_version_ 1654960036759207936
score 13.211869

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