Cover Image

Shoot induction of abrus precatorius

Abrus precatorius is valuable herb due to its medicinal properties. To date, there are several shoot regeneration studies of local A. precatorius have been reported. However, percentage of shoot regenerated from nodal explants was very low (70%). Therefore, the study aimed to investigate surface-ste...

Full description

Saved in:
Bibliographic Details
Main Author: Aziz, Nur Azian
Format: Thesis
Published: 2018
Subjects:
Q Science (General)
Online Access:http://eprints.utm.my/id/eprint/81499/
http://dms.library.utm.my:8080/vital/access/manager/Repository/vital:120472
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.utm.81499
record_format eprints
spelling my.utm.814992019-08-23T05:19:14Z http://eprints.utm.my/id/eprint/81499/ Shoot induction of abrus precatorius Aziz, Nur Azian Q Science (General) Abrus precatorius is valuable herb due to its medicinal properties. To date, there are several shoot regeneration studies of local A. precatorius have been reported. However, percentage of shoot regenerated from nodal explants was very low (70%). Therefore, the study aimed to investigate surface-sterilization method, seed germination percentage and shoot induction percentage of A. precatorius. The seeds were surface-sterilized with different concentrations of Clorox (v/v) (30%, 60% and 100%) and the percentage of seed germination on different MS strength (full, half and quarter) were also investigated. Nodal explants were treated with different concentrations of TDZ and BAP to induce shoot. The results showed that 100% survived explants was obtained at 100% (v/v) Clorox. For seed germination, the highest germination percentage was achieved when seeds cultured at half strength MS (96%). The result also indicated that both full MS and half MS had better percentage of seed germination than quarter MS. For shoot induction assessment, the highest shoot induction (100% shoot induction percentage) was achieved when nodal explants were placed on MS fortified with 0.5 mg/L TDZ with 3.05 shoots per explant. In comparison, 0.5 mg/L TDZ gave higher shoot induction percentage than 2.5 mg/L BAP. In conclusion, TDZ produced higher percentage of shoot induction than BAP from nodal segments of A. precatorius. 2018 Thesis NonPeerReviewed Aziz, Nur Azian (2018) Shoot induction of abrus precatorius. Masters thesis, Universiti Teknologi Malaysia. http://dms.library.utm.my:8080/vital/access/manager/Repository/vital:120472
institution Universiti Teknologi Malaysia
building UTM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Malaysia
content_source UTM Institutional Repository
url_provider http://eprints.utm.my/
topic Q Science (General)
spellingShingle Q Science (General)
Aziz, Nur Azian
Shoot induction of abrus precatorius
description Abrus precatorius is valuable herb due to its medicinal properties. To date, there are several shoot regeneration studies of local A. precatorius have been reported. However, percentage of shoot regenerated from nodal explants was very low (70%). Therefore, the study aimed to investigate surface-sterilization method, seed germination percentage and shoot induction percentage of A. precatorius. The seeds were surface-sterilized with different concentrations of Clorox (v/v) (30%, 60% and 100%) and the percentage of seed germination on different MS strength (full, half and quarter) were also investigated. Nodal explants were treated with different concentrations of TDZ and BAP to induce shoot. The results showed that 100% survived explants was obtained at 100% (v/v) Clorox. For seed germination, the highest germination percentage was achieved when seeds cultured at half strength MS (96%). The result also indicated that both full MS and half MS had better percentage of seed germination than quarter MS. For shoot induction assessment, the highest shoot induction (100% shoot induction percentage) was achieved when nodal explants were placed on MS fortified with 0.5 mg/L TDZ with 3.05 shoots per explant. In comparison, 0.5 mg/L TDZ gave higher shoot induction percentage than 2.5 mg/L BAP. In conclusion, TDZ produced higher percentage of shoot induction than BAP from nodal segments of A. precatorius.
format Thesis
author Aziz, Nur Azian
author_facet Aziz, Nur Azian
author_sort Aziz, Nur Azian
title Shoot induction of abrus precatorius
title_short Shoot induction of abrus precatorius
title_full Shoot induction of abrus precatorius
title_fullStr Shoot induction of abrus precatorius
title_full_unstemmed Shoot induction of abrus precatorius
title_sort shoot induction of abrus precatorius
publishDate 2018
url http://eprints.utm.my/id/eprint/81499/
http://dms.library.utm.my:8080/vital/access/manager/Repository/vital:120472
_version_ 1643658728697757696
score 13.211869

Similar Items