Staff View: In vitro and in vivo biological screening of kefiran polysaccharide produced by Lactobacillus kefiranofaciens

In vitro and in vivo biological screening of kefiran polysaccharide produced by Lactobacillus kefiranofaciens

Kefiran is a functional fermented milk product traditionally used for its beneficial probiotic properties. It exhibits antimicrobial, antioxidant, anti-inflammatory anticancer and different health promoting characteristics. Although kefiran showed potential effects against many cancer cell lines, li...

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Main Authors:	Elsayed, E. A., Farooq, M., Dailin, D., El-Enshasy, H. A., Othman, N. Z., Malek, R., Danial, E., Wadaan, M.
Format:	Article
Published:	Scientific Publishers of India 2017
Subjects:	TP Chemical technology
Online Access:	http://eprints.utm.my/id/eprint/80268/
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spelling	my.utm.802682019-04-25T01:24:40Z http://eprints.utm.my/id/eprint/80268/ In vitro and in vivo biological screening of kefiran polysaccharide produced by Lactobacillus kefiranofaciens Elsayed, E. A. Farooq, M. Dailin, D. El-Enshasy, H. A. Othman, N. Z. Malek, R. Danial, E. Wadaan, M. TP Chemical technology Kefiran is a functional fermented milk product traditionally used for its beneficial probiotic properties. It exhibits antimicrobial, antioxidant, anti-inflammatory anticancer and different health promoting characteristics. Although kefiran showed potential effects against many cancer cell lines, little information is present in the literature on its effect against cervical and hepatocellular carcinoma as well as on zebrafish embryos. The study aimed at investigating the cytotoxicity (in human cervical and hepatocellular carcinoma cell lines) and developmental toxicity (in zebrafish embryos) of kefiran produced by the fermentation of Lactobacillus kefiranofaciens. Cervical and hepatocellular cancer cells were exposed to serial concentrations of kefiran to evaluate its cytotoxic activities. Further biological effects of kefiran on the mortality and developmental abnormalities of zebrafish embryos were investigated. Results showed that kefiran significantly affected the viability of both tested cancer cell lines in a dose-dependent manner with IC50 values of 358.8 ± 1.65 and 413.5 ± 1.05 µg/ml for HeLa and HepG2 cells, respectively. Furthermore, kefiran adversely affected the morphological characteristics of the cells. Kefiran extract was much safer for zebrafish embryos and no mortality was observed up to 100 µg/ml, whereas the LC50 value (≥ 279.76 µg/ml) was also very high. Moreover, no developmental toxicity was observed up to 100 µg/ml concentration. Conclusively, microbially-produced kefiran showed anticancer properties in two tested human cancer cells, while its safer profiles in animals (zebrafish embryos) poses it as potential anticancer agent which does not affect normal tissue growth. Scientific Publishers of India 2017 Article PeerReviewed Elsayed, E. A. and Farooq, M. and Dailin, D. and El-Enshasy, H. A. and Othman, N. Z. and Malek, R. and Danial, E. and Wadaan, M. (2017) In vitro and in vivo biological screening of kefiran polysaccharide produced by Lactobacillus kefiranofaciens. Biomedical Research (India), 28 (2). pp. 594-600. ISSN 0970938X
institution	Universiti Teknologi Malaysia
building	UTM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Teknologi Malaysia
content_source	UTM Institutional Repository
url_provider	http://eprints.utm.my/
topic	TP Chemical technology
spellingShingle	TP Chemical technology Elsayed, E. A. Farooq, M. Dailin, D. El-Enshasy, H. A. Othman, N. Z. Malek, R. Danial, E. Wadaan, M. In vitro and in vivo biological screening of kefiran polysaccharide produced by Lactobacillus kefiranofaciens
description	Kefiran is a functional fermented milk product traditionally used for its beneficial probiotic properties. It exhibits antimicrobial, antioxidant, anti-inflammatory anticancer and different health promoting characteristics. Although kefiran showed potential effects against many cancer cell lines, little information is present in the literature on its effect against cervical and hepatocellular carcinoma as well as on zebrafish embryos. The study aimed at investigating the cytotoxicity (in human cervical and hepatocellular carcinoma cell lines) and developmental toxicity (in zebrafish embryos) of kefiran produced by the fermentation of Lactobacillus kefiranofaciens. Cervical and hepatocellular cancer cells were exposed to serial concentrations of kefiran to evaluate its cytotoxic activities. Further biological effects of kefiran on the mortality and developmental abnormalities of zebrafish embryos were investigated. Results showed that kefiran significantly affected the viability of both tested cancer cell lines in a dose-dependent manner with IC50 values of 358.8 ± 1.65 and 413.5 ± 1.05 µg/ml for HeLa and HepG2 cells, respectively. Furthermore, kefiran adversely affected the morphological characteristics of the cells. Kefiran extract was much safer for zebrafish embryos and no mortality was observed up to 100 µg/ml, whereas the LC50 value (≥ 279.76 µg/ml) was also very high. Moreover, no developmental toxicity was observed up to 100 µg/ml concentration. Conclusively, microbially-produced kefiran showed anticancer properties in two tested human cancer cells, while its safer profiles in animals (zebrafish embryos) poses it as potential anticancer agent which does not affect normal tissue growth.
format	Article
author	Elsayed, E. A. Farooq, M. Dailin, D. El-Enshasy, H. A. Othman, N. Z. Malek, R. Danial, E. Wadaan, M.
author_facet	Elsayed, E. A. Farooq, M. Dailin, D. El-Enshasy, H. A. Othman, N. Z. Malek, R. Danial, E. Wadaan, M.
author_sort	Elsayed, E. A.
title	In vitro and in vivo biological screening of kefiran polysaccharide produced by Lactobacillus kefiranofaciens
title_short	In vitro and in vivo biological screening of kefiran polysaccharide produced by Lactobacillus kefiranofaciens
title_full	In vitro and in vivo biological screening of kefiran polysaccharide produced by Lactobacillus kefiranofaciens
title_fullStr	In vitro and in vivo biological screening of kefiran polysaccharide produced by Lactobacillus kefiranofaciens
title_full_unstemmed	In vitro and in vivo biological screening of kefiran polysaccharide produced by Lactobacillus kefiranofaciens
title_sort	in vitro and in vivo biological screening of kefiran polysaccharide produced by lactobacillus kefiranofaciens
publisher	Scientific Publishers of India
publishDate	2017
url	http://eprints.utm.my/id/eprint/80268/
_version_	1643658363828961280
score	13.211869

In vitro and in vivo biological screening of kefiran polysaccharide produced by Lactobacillus kefiranofaciens

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