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Performance of microwave assisted aqueous enzymatic technique for elateriospermum tapos seed oil extraction

Green, sustainable and effective extraction method is currently in demand for extraction of valuable compounds from natural resources. In this study, microwave assisted aqueous enzymatic extraction (MAAEE) which uses an electromagnetic wave as heating medium incorporated with non-toxic enzymatic aqu...

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Main Author: Hadi Tan, Nuraimi Azlan
Format: Thesis
Language:English
Published: 2017
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TP Chemical technology
Online Access:http://eprints.utm.my/id/eprint/79457/1/NuraimiAzlanPFChE2017.pdf
http://eprints.utm.my/id/eprint/79457/
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spelling my.utm.794572018-10-31T12:39:26Z http://eprints.utm.my/id/eprint/79457/ Performance of microwave assisted aqueous enzymatic technique for elateriospermum tapos seed oil extraction Hadi Tan, Nuraimi Azlan TP Chemical technology Green, sustainable and effective extraction method is currently in demand for extraction of valuable compounds from natural resources. In this study, microwave assisted aqueous enzymatic extraction (MAAEE) which uses an electromagnetic wave as heating medium incorporated with non-toxic enzymatic aqueous solution as solvent was applied to extract omega-3 (ω-3) rich oil from Elateriospermum tapos seed. The effect of parameters in the MAAEE process including optimization, oil characterization, and mass transfer models relationship were investigated. In this study, experiments were carried out based on the design of experiment by central composite design using the design expert software. The optimization of extraction was analyzed by the response surface methodology and mass transfer models which were correlated with the models of the modified Fick’s law, Patricelli’s model and mass balance based on broken and intact cells. The results revealed that low microwave power (110 W) and small particle size (0.5 mm) gave significant effect on the extraction yield whereas increasing concentration of enzyme cocktail from 1 to 3% significantly increased the concentration of extracted ω-3 fatty acid. The optimum conditions were determined to be 110 W microwave power, 30 second extraction time, 1% enzyme cocktail concentration and 0.5 mm particle size, resulted in 46.12 ± 1.48% recovery of extraction. Meanwhile, the optimum ω-3 fatty acid concentration was achieved at microwave power of 550 W, extraction time of 75 second, enzyme cocktail concentration of 3% and particle size of 0.5 mm resulted into 348.96 ± 24.88 mg ω-3/g extracted oil compared to 106.57 ± 4.32 mg ω-3/g of oil from Soxhlet extraction. The modeling study indicated mass balance model based on broken and intact cells as the best fitted model which gave the highest value of R2 (0.9932) and lower value of absolute average relative deviation (3.7983) at 550 W of microwave power. Thus, it was proven that MAAEE was able to accelerate the extraction process and provided high quality of ω-3 rich oil extract at the same time. 2017 Thesis NonPeerReviewed application/pdf en http://eprints.utm.my/id/eprint/79457/1/NuraimiAzlanPFChE2017.pdf Hadi Tan, Nuraimi Azlan (2017) Performance of microwave assisted aqueous enzymatic technique for elateriospermum tapos seed oil extraction. PhD thesis, Universiti Teknologi Malaysia, Faculty of Chemical & Energy Engineering.
institution Universiti Teknologi Malaysia
building UTM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Teknologi Malaysia
content_source UTM Institutional Repository
url_provider http://eprints.utm.my/
language English
topic TP Chemical technology
spellingShingle TP Chemical technology
Hadi Tan, Nuraimi Azlan
Performance of microwave assisted aqueous enzymatic technique for elateriospermum tapos seed oil extraction
description Green, sustainable and effective extraction method is currently in demand for extraction of valuable compounds from natural resources. In this study, microwave assisted aqueous enzymatic extraction (MAAEE) which uses an electromagnetic wave as heating medium incorporated with non-toxic enzymatic aqueous solution as solvent was applied to extract omega-3 (ω-3) rich oil from Elateriospermum tapos seed. The effect of parameters in the MAAEE process including optimization, oil characterization, and mass transfer models relationship were investigated. In this study, experiments were carried out based on the design of experiment by central composite design using the design expert software. The optimization of extraction was analyzed by the response surface methodology and mass transfer models which were correlated with the models of the modified Fick’s law, Patricelli’s model and mass balance based on broken and intact cells. The results revealed that low microwave power (110 W) and small particle size (0.5 mm) gave significant effect on the extraction yield whereas increasing concentration of enzyme cocktail from 1 to 3% significantly increased the concentration of extracted ω-3 fatty acid. The optimum conditions were determined to be 110 W microwave power, 30 second extraction time, 1% enzyme cocktail concentration and 0.5 mm particle size, resulted in 46.12 ± 1.48% recovery of extraction. Meanwhile, the optimum ω-3 fatty acid concentration was achieved at microwave power of 550 W, extraction time of 75 second, enzyme cocktail concentration of 3% and particle size of 0.5 mm resulted into 348.96 ± 24.88 mg ω-3/g extracted oil compared to 106.57 ± 4.32 mg ω-3/g of oil from Soxhlet extraction. The modeling study indicated mass balance model based on broken and intact cells as the best fitted model which gave the highest value of R2 (0.9932) and lower value of absolute average relative deviation (3.7983) at 550 W of microwave power. Thus, it was proven that MAAEE was able to accelerate the extraction process and provided high quality of ω-3 rich oil extract at the same time.
format Thesis
author Hadi Tan, Nuraimi Azlan
author_facet Hadi Tan, Nuraimi Azlan
author_sort Hadi Tan, Nuraimi Azlan
title Performance of microwave assisted aqueous enzymatic technique for elateriospermum tapos seed oil extraction
title_short Performance of microwave assisted aqueous enzymatic technique for elateriospermum tapos seed oil extraction
title_full Performance of microwave assisted aqueous enzymatic technique for elateriospermum tapos seed oil extraction
title_fullStr Performance of microwave assisted aqueous enzymatic technique for elateriospermum tapos seed oil extraction
title_full_unstemmed Performance of microwave assisted aqueous enzymatic technique for elateriospermum tapos seed oil extraction
title_sort performance of microwave assisted aqueous enzymatic technique for elateriospermum tapos seed oil extraction
publishDate 2017
url http://eprints.utm.my/id/eprint/79457/1/NuraimiAzlanPFChE2017.pdf
http://eprints.utm.my/id/eprint/79457/
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score 13.211869

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